scholarly journals Hapten Synthesis and the Development of an Ultrasensitive Indirect Competitive ELISA for the Determination of Diethylstilbestrol in Food Samples

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xingdong Yang ◽  
Yinbiao Wang ◽  
Chunmei Song ◽  
Xiaofei Hu ◽  
Fangyu Wang ◽  
...  
Keyword(s):  
Food Samples ◽  
Competitive Elisa ◽  
Indirect Competitive Elisa ◽  
Hapten Synthesis

Detection of total bile acids in biological samples using an indirect competitive ELISA based on four monoclonal antibodies

2017 ◽  
Vol 9 (4) ◽  
pp. 625-633 ◽  
Author(s):  
Shuchen Liu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Gaofeng Qin ◽  
Jinjun Cheng ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Clinical Practice ◽  
Biological Samples ◽  
Competitive Elisa ◽  
Routine Clinical Practice ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Competitive Elisa ◽  
Different Types ◽  
Total Bile Acids

We investigated a newly developed indirect competitive enzyme-linked immunosorbent assay for the determination of 5 major components of TBA, which works efficiently in different types of biological samples, and may be suitable for routine clinical practice.

Determination of Florfenicol Amine Residues in Animal Edible Tissues by an Indirect Competitive ELISA

2008 ◽  
Vol 56 (18) ◽  
pp. 8261-8267 ◽  
Author(s):  
Jin-E Wu ◽  
Chao Chang ◽  
Wen-Ping Ding ◽  
Dong-Ping He
Keyword(s):  
Competitive Elisa ◽  
Indirect Competitive Elisa ◽  
Edible Tissues

scholarly journals Development of indirect competitive ELISA for determination of dehydroabietic acid in duck skin and comparison with the HPLC method

2020 ◽  
Vol 99 (6) ◽  
pp. 3280-3285
Author(s):  
Xinyuan Qiu ◽  
Jingjing Ma ◽  
Pengpeng Li ◽  
Zhiming Geng ◽  
Chong Sun ◽  
...  
Keyword(s):  
Dehydroabietic Acid ◽  
Hplc Method ◽  
Competitive Elisa ◽  
Indirect Competitive Elisa

Optimization and Validation of an Indirect Competitive ELISA for Detecting Enrofloxacin Residue

2012 ◽  
Vol 424-425 ◽  
pp. 603-606
Author(s):  
Shi Xiu Wu ◽  
Jin Qing Jiang
Keyword(s):  
Polyclonal Antibody ◽  
Correlation Coefficients ◽  
Competitive Elisa ◽  
Linear Range ◽  
Elisa System ◽  
Indirect Competitive Elisa ◽  
Chicken Muscle ◽  
Ic50 Values ◽  
Liver And Kidney

This article aimed to optimize a polyclonal antibody based indirect competitive ELISA (icELISA) and compare between the icELSIA method and the LC-MS technology for the determination of enrofloxacin (ENR) residue. Based on the square matrix titration, linear range of the icELISA was from 0.006 to 31.5 ng/mL, with LOD and IC50 values of 0.003 ng/mL and 0.45 ng/mL, respectively. After optimization, 0.03 mol/L of HCl was used in the assay buffer and this ELISA system can tolerate acetonitrile not higher than 10%. The recoveries of ENR were in the range of 98.3-127.5%, 85.7-112.5% and 97.4-103.8% for chicken muscle, liver and kidney. The correlation coefficients (R2) of the ELISA and LC-MS data were 0.9472 in muscle, 0.9843 in liver, and 0.9382 in kidney, respectively.

AN INDIRECT COMPETITIVE ELISA FOR DETERMINATION OF CITRININ

2011 ◽  
Vol 31 (4) ◽  
pp. 497-504 ◽  
Author(s):  
YONGNING LI ◽  
YUANYUAN WANG ◽  
YANGHAO GUO
Keyword(s):  
Competitive Elisa ◽  
Indirect Competitive Elisa

A sensitive and group-specific monoclonal antibody-based indirect competitive ELISA for the determination of salbutamol in swine meat and liver samples

2017 ◽  
Vol 9 (39) ◽  
pp. 5806-5815 ◽  
Author(s):  
Yin Yuan ◽  
Yingying Zhao ◽  
Kang Wu ◽  
Hong Yang ◽  
Kang Zhao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Harmful Effect ◽  
Livestock Production ◽  
Competitive Elisa ◽  
Specific Monoclonal Antibody ◽  
Animal Feeds ◽  
Indirect Competitive Elisa

The use of salbutamol (SAL) has been prohibited as an additive in animal feeds for livestock production in many countries due to its harmful effect to the consumers.

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