Feed Additive Residues, Determination of 3-Amino-5-nitro-o-toluamide (ANOT) in Chicken Tissues

1961 ◽  
Vol 9 (3) ◽  
pp. 201-204 ◽  
Author(s):  
B. J. Thiegs ◽  
G. N. Smith ◽  
J. L. Bevirt
Keyword(s):  
Feed Additive ◽  
Chicken Tissues ◽  
Residues Determination

Improved Method for Gas-Liquid Chromatographic Determination of Clopidol in Chicken Tissues

1980 ◽  
Vol 63 (6) ◽  
pp. 1211-1214
Author(s):  
Etsuko Suzuki ◽  
Mihoko Matsuda ◽  
Atsushi Momose ◽  
Masaya Namekata
Keyword(s):  
Exchange Resin ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Alumina Column ◽  
Resin Column ◽  
Chicken Tissues ◽  
Liquid Chromatographic Determination ◽  
Low Levels ◽  
Liquid Chromatographic

Abstract A sensitive and specific gas-liquid chromatographic (GLC) method has been developed for determining low levels of clopidol in chicken tissues. Clopidol is extracted from the tissues with methanol, and cleaned up on an alumina column and an anion exchange resin column with 0.1% acetic acid–methanol as eluate. Clopidol is methylated with diazomethane, and then determined by GLC. 2,4-Dinitro-l-chlorobenzene is used as an internal standard. The method is applicable to levels as low as 2 ppb in chicken tissues. Recoveries of 2–20 ppb clopidol added to tissues averaged 87% for muscle, 84% for liver, 80% for kidney, and 76% for fat.

scholarly journals LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

2014 ◽  
Vol 58 (4) ◽  
pp. 573-579 ◽  
Author(s):  
Anna Gajda ◽  
Andrzej Posyniak ◽  
Grzegorz Tomczyk
Keyword(s):  
Oral Administration ◽  
Broiler Chickens ◽  
Thigh Muscle ◽  
Limit Of Quantification ◽  
Limit Values ◽  
Chicken Tissues ◽  
Short Period ◽  
Liquid Chromatography Tandem Mass ◽  
Liver And Kidney

Abstract For the purpose of quantitative determination of doxycycline (DC) residues in tissues, a sensitive liquid chromatography - tandem mass spectrometry (LC-MS/MS) method was developed. The method was used to determine DC residues in chicken tissues (breast and thigh muscle, liver and kidney) after oral administration with drinking water to five-weak-old broiler chickens. The DC was administered for five consecutive days at a therapeutic dose of 10 mg/kg b.w. once a day. The tissues were collected after 6 h, 24 h, 7 d, and 8 d. The method was validated and the decision limit was established for muscle - 109.2 μg/kg, for liver - 326.1 μg/kg, and for kidney - 634.0 μg/kg. The detection limit was 2 μg/kg and the limit of quantification was 5 μg/kg. In a short period after ceasing the treatment, the detected concentrations of DC were much higher than the established maximum residue limit values. The highest residue concentrations of DC were observed in the kidney, followed by the liver and muscle. The lowest concentration of DC was determined in tight muscle.

Determination of Norfloxacin in Chicken Tissues by HPLC with Fluorescence Detection

2005 ◽  
Vol 28 (1) ◽  
pp. 121-135 ◽  
Author(s):  
C. Kowalski ◽  
Z. Roliński ◽  
T. Sławik ◽  
B. K. Głód
Keyword(s):  
Fluorescence Detection ◽  
Chicken Tissues

Application of the CEN/ISO Standard for Phytase Activity Measurements to a New Phytase Product: Determination of a Robust Conversion Factor by an Interlaboratory Study for Screening Feed Samples

2019 ◽  
Vol 102 (6) ◽  
pp. 1808-1813
Author(s):  
María José González de la Huebra ◽  
Piotr Robouch ◽  
Håkan Emteborg ◽  
Stefano Bellorini ◽  
Aneta Cizek-Stroh ◽  
...  
Keyword(s):  
Conversion Factor ◽  
Feed Additives ◽  
Phytase Activity ◽  
Feed Additive ◽  
The European Union ◽  
Iso Standard ◽  
Official Control ◽  
Activity Measurements ◽  
Feed Samples

Abstract Background: Phytase-based preparations are important feed additives currently authorised in the European Union (EU). The European Standard (EN) and International Organization for Standardization (ISO) standard 30024 describes a harmonized method for the determination of phytase activity and is fit-for-purpose for official control of a group of phytase products. However, it is not suitable for the determination of the phytase activity of a new feed additive encoded as 4a16 in the EU Register of Feed Additives, to which a slightly different phytase activity definition has been attributed. Objective: To establish a robust conversion factor to support official control laboratories that apply the EN ISO method when monitoring feed products containing 4a16. Methods: The phytase activity of test materials was determined by the participants using the EN ISO and/or the “applicant” methods. Results: Robust relative SDs for repeatability and for reproducibility of the methods applied for the determination of the phytase activity in the materials containing the 4a16 feed additive ranged from 2.6 to 22% (EN ISO method) and from 2.4 to 39% (applicant method). Conclusions: The data obtained confirmed the performance characteristics published for other phytase-based feeds in the related standard methods. These results allowed us to estimate a factor of 2.68 to convert phytase activities measured with the EN ISO method into the enzyme activity measured with the applicant method. Highlights: The obtained conversion factor will allow EU official laboratories to screen feed samples supplemented with the 4a16 phytase by applying EN ISO Standard 30024.

Determination of Monensin in Chicken Tissues by Liquid Chromatography with Postcolumn Derivatization

1994 ◽  
Vol 77 (4) ◽  
pp. 885-890 ◽  
Author(s):  
John W Moran ◽  
J Matthew Rodewald ◽  
Alvin L Donoho ◽  
Mark R Coleman
Keyword(s):  
Liquid Chromatography ◽  
Silica Gel ◽  
Chicken Tissues ◽  
Chicken Muscle ◽  
Limit Of Quantitation ◽  
Microbiological Techniques ◽  
Wavelength Detector ◽  
Acidic Conditions ◽  
Postcolumn Derivatization

Abstract A method is described for the detection and quantitation of monensin in chicken tissues by liquid chromatography with postcolumn derivatization with vanillin. Monensin is extracted from the tissues by homogenization with methanol–water and is isolated and concentrated by liquid–liquid partition and sorbent extraction with silica gel. Monensin is mixed postcolumn with vanillin under acidic conditions and heated, and the resulting products are measured by a variable-wavelength detector operating at 520 nm. The method has a limit of quantitation of 0.025 μg/g and is validated for use in the analyses of chicken muscle, liver, and skin (with adhering fat tissues) for monensin. Standard recoveries from the 3 tissue types tested at 3 levels ranged from 82 to 96%. The method represents an improvement in specificity, accuracy, and analysis time over existing methods, which use microbiological techniques.

Liquid Chromatographic Determination of Spiramycin Residues in Chicken Tissues

1986 ◽  
Vol 69 (4) ◽  
pp. 644-646
Author(s):  
Tomoko Nagata ◽  
Masanobu Saeki
Keyword(s):  
Detection Limit ◽  
Ethyl Ether ◽  
Chromatographic Determination ◽  
Chicken Tissues ◽  
Chicken Muscles ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for determination of spiramycin residues in chicken muscles. The drug is extracted from muscles with acetonitrile, the extract is concentrated to 3-4 mL and rinsed with n-hexane followed by ethyl ether, and the drug is extracted with chloroform. LC analysis is carried out on a Zorbax BP-C8 column, and spiramycin is detected spectrophotometrically at 231 nm. Recoveries of spiramycin added to chicken muscles at 0.2 and 0.1 ppm were 93.9 and 89.0%, respectively. The detection limit was 5 ng for spiramycin standard, and 0.05 ppm in chicken muscles.

Sign in / Sign up

Export Citation Format

Share Document