Total Phenolic Content, Radical Scavenging Properties, and Essential Oil Composition ofOriganumSpecies from Different Populations

2010 ◽  
Vol 58 (2) ◽  
pp. 1115-1120 ◽  
Author(s):  
José S. Dambolena ◽  
María P. Zunino ◽  
Enrique I. Lucini ◽  
Rubén Olmedo ◽  
Erika Banchio ◽  
...  
Author(s):  
Özlem Aras Aşcı ◽  
Hikmet Deveci ◽  
Alican Erdeğer ◽  
Kübra Nur Özdemir ◽  
Tunhan Demirci ◽  
...  

This study was aimed to determine the effects of 24-eBL, a steroid growth regulator, on the plant growth, total phenolic content, essential oil content and composition in “Munstead” lavender cultivar belonging to Lavandula angustifolia. For this aim, 24-eBL was applied to plants at four different concentrations (0, 0.75, 1.50 and 2.25 mg/l) twice, at the beginning of budding and 10 days after the first application. The plants harvested during the full bloom period were evaluated for fresh and dry stemmed flower yields, stemless dry flower yield, total phenolic content and essential oil yield and composition. As a result of the study, 24-eBL at concentrations of 0.75 and 1.50 mg/l significantly increased the stemless dry flower yield, total phenolic content and essential oil yield per plant. 24-eBL applications also changed essential oil composition. To conclude, 24-eBL applications can be used to increase the dry flower yield, phenolic and essential oil contents in lavender plants.


Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 717-727
Author(s):  
Hulya Dogan

Present study reports a comparative screening of three ecotypes of Ziziphora tenuior L., sampled from three region with different elevation in middle and northeastern Turkey, based on their essential oil composition, total phenolic content and antioxidant capacities. Z. tenuior ecotypes showed twenty compounds, representing 89.18-91.44% of the essential oil (EOs) identified by GC-MS. The major components of EOs in ecotypes were pulegone (37.23-49.12%), and followed by 1,8-Cineole (2.26-7.78%), limonene (4.20-5.44%), ?-Caryophyllene (3.88-5.11%) and thymol (2.68-4.11%), respectively. Ecotypes showed variable pulegone content and it was increased with elevation increase. The total phenolic contents varied among ecotypes ranged from 21.13 to 27.50 mg of gallic acid equivalent/g EOs. Antioxidant capacity was determined in vitro using DPPH radical-scavenging and FRAP (Ferric reducing power) assays and expressed as concentration of each extract required to inhibit radical by 50% (IC50 and EC50) values that ranged from 0.80 to 0.97 mg/ml in DPPH and 0.42 to 0.55 mg/ml in FRAP assays, respectively. Our results indicated that antioxidant capacity changed among ecotypes. Taking into account the essential oil content, it seems that to select ecotype was the most appropriate to obtain more pulegone for this plant from its wild habitat.


2015 ◽  
Vol 70 (3-4) ◽  
pp. 51-58 ◽  
Author(s):  
Ardalan Alizadeh

Abstract Satureja rechingeri is a rare endemic and endangered species found in Iran. Its propagation, variations in essential oil and phenolic content, as well as antioxidant and antimicrobial activities at different phenological stages are reported in this study. The chemical composition of essential oils obtained by hydro-distillation from the aerial parts were determined by GC and GC-MS. A total of 47 compounds were identified in the essential oils of S. rechingeri at different phenological stages. The major components of all oils were carvacrol (83.6%–90.4%), p-cymene (0.8%–2.9%) and γ-terpinene (0.6%–2.4%). The total phenolic content and the antioxidant activity of methanolic extracts were determined with the Folin-Ciocalteau reagent and by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) assay, respectively. Total phenols varied from 35.5 to 37.5 mg gallic acid equivalents/g dry weight (dw), and IC50 values in the radical scavenging assay ranged from 46.2 to 50.2 mg/mL, while those in the FRAP assay were between 49.6 and 52.5 μM quercetin equivalents/g dw. By the disc diffusion method and by determination of the minimum inhibitory concentrations (MIC), the essentials oils of the various phenological stages were found to have high activities against four medically important pathogens.


Author(s):  
JEYAKANI M ◽  
RAJALAKSHMI M

Objectives: Essential oil and their compounds are getting increasing interest due to their multipurpose functional as alternatives to artificial preservatives. The aim of this study was focused to comparative analyses on hydrodistillation, total phenolic content (TPC), and antioxidant activity of essential oil derived from Melaleuca alternifolia (M. alternifolia) leaves procured from natural leaves, in-vitro leaves, and callus sources. Methods: The essential oil was extracted using hydrodistillation. The Folin-Ciocalteu method was used to determine the TPC equivalent and antioxidant activity of essential oils. Results: TPC of essential oil from in-vitro sources of leaves contained 14.79 mg gallic acid equivalent/g dry plant material and its IC50 value was found to be 70% in 1-diphenyl 2-picrylhyorazyl assay. Thus, this source of essential oil showed good free radical scavenging activity. Conclusion: The estimated biological potential was obtained in the essential oil from in-vitro sources of leaves M. alternifolia.


Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential


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