scholarly journals Correlation between Desorption Force Measured by Atomic Force Microscopy and Adsorption Free Energy Measured by Surface Plasmon Resonance Spectroscopy for Peptide−Surface Interactions

Langmuir ◽  
2010 ◽  
Vol 26 (24) ◽  
pp. 18852-18861 ◽  
Author(s):  
Yang Wei ◽  
Robert A. Latour
Keyword(s):  
Atomic Force Microscopy ◽  
Free Energy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Surface Interactions ◽  
Resonance Spectroscopy ◽  
Surface Plasmon Resonance Spectroscopy ◽  
Force Microscopy ◽  
Adsorption Free Energy ◽  
Atomic Force

scholarly journals Lectins at Interfaces—An Atomic Force Microscopy and Multi-Parameter-Surface Plasmon Resonance Study

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2348 ◽  
Author(s):  
Katrin Niegelhell ◽  
Thomas Ganner ◽  
Harald Plank ◽  
Evelyn Jantscher-Krenn ◽  
Stefan Spirk
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Protein Adsorption ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Spectroscopy ◽  
Carbohydrate Binding ◽  
Force Microscopy ◽  
Atomic Force ◽  
Slow Kinetics

Lectins are a diverse class of carbohydrate binding proteins with pivotal roles in cell communication and signaling in many (patho)physiologic processes in the human body, making them promising targets in drug development, for instance, in cancer or infectious diseases. Other applications of lectins employ their ability to recognize specific glycan epitopes in biosensors and glycan microarrays. While a lot of research has focused on lectin interaction with specific carbohydrates, the interaction potential of lectins with different types of surfaces has not been addressed extensively. Here, we screen the interaction of two specific plant lectins, Concanavalin A and Ulex Europaeus Agglutinin-I with different nanoscopic thin films. As a control, the same experiments were performed with Bovine Serum Albumin, a widely used marker for non-specific protein adsorption. In order to test the preferred type of interaction during adsorption, hydrophobic, hydrophilic and charged polymer films were explored, such as polystyrene, cellulose, N,-N,-N-trimethylchitosan chloride and gold, and characterized in terms of wettability, surface free energy, zeta potential and morphology. Atomic force microscopy images of surfaces after protein adsorption correlated very well with the observed mass of adsorbed protein. Surface plasmon resonance spectroscopy studies revealed low adsorbed amounts and slow kinetics for all of the investigated proteins for hydrophilic surfaces, making those resistant to non-specific interactions. As a consequence, they may serve as favorable supports for biosensors, since the use of blocking agents is not necessary.

Probing protein–peptide–protein molecular architecture by atomic force microscopy and surface plasmon resonance

The Analyst ◽  
2000 ◽  
Vol 125 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Molly M. Stevens ◽  
Stephanie Allen ◽  
Martyn C. Davies ◽  
Clive J. Roberts ◽  
Saul J. B. Tendler ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Architecture ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Nanostructured Chitosan/Maghemite Composites Thin Film for Potential Optical Detection of Mercury Ion by Surface Plasmon Resonance Investigation

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1497
Author(s):  
Nurul Illya Muhamad Fauzi ◽  
Yap Wing Fen ◽  
Nur Alia Sheh Omar ◽  
Silvan Saleviter ◽  
Wan Mohd Ebtisyam Mustaqim Mohd Daniyal ◽  
...  
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mercury Ion ◽  
Resonance Spectroscopy ◽  
Force Microscopy ◽  
Sensing Applications ◽  
Atomic Force ◽  
Fe2o3 Thin Film

In this study, synthesis and characterization of chitosan/maghemite (Cs/Fe2O3) composites thin film has been described. Its properties were characterized using Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and ultraviolet-visible spectroscopy (UV-Vis). FTIR confirmed the existence of Fe–O bond, C–N bond, C–C bond, C–O bond, O=C=O bond and O–H bond in Cs/Fe2O3 thin film. The surface morphology of the thin film indicated the relatively smooth and homogenous thin film, and also confirmed the interaction of Fe2O3 with the chitosan. Next, the UV-Vis result showed high absorbance value with an optical band gap of 4.013 eV. The incorporation of this Cs/Fe2O3 thin film with an optical-based method, i.e., surface plasmon resonance spectroscopy showed positive response where mercury ion (Hg2+) can be detected down to 0.01 ppm (49.9 nM). These results validate the potential of Cs/Fe2O3 thin film for optical sensing applications in Hg2+ detection.

Degradation of a Thin Polymer Film Studied by Simultaneous in Situ Atomic Force Microscopy and Surface Plasmon Resonance Analysis

1995 ◽  
Vol 99 (29) ◽  
pp. 11537-11542 ◽  
Author(s):  
X. Chen ◽  
K. M. Shakesheff ◽  
M. C. Davies ◽  
J. Heller ◽  
C. J. Roberts ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Thin Polymer Film ◽  
Surface Plasmon Resonance Analysis ◽  
Force Microscopy ◽  
Resonance Analysis ◽  
Atomic Force ◽  
Thin Polymer

scholarly journals Membrane binding of β2-glycoprotein I can be described by a two-state reaction model: an atomic force microscopy and surface plasmon resonance study

2005 ◽  
Vol 389 (3) ◽  
pp. 665-673 ◽  
Author(s):  
Roland Gamsjaeger ◽  
Alexander Johs ◽  
Anna Gries ◽  
Hermann J. Gruber ◽  
Christoph Romanin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Electrostatic Interactions ◽  
Reaction Model ◽  
Force Microscopy ◽  
Glycoprotein I ◽  
Atomic Force ◽  
Acidic Phospholipids

Complexes formed between β2GPI (β2-glycoprotein I), a human plasma protein, and biological membranes are considered to be targets of macrophages and antiphospholipid autoantibodies involved in autoimmune diseases, such as antiphospholipid syndrome or systemic lupus erythematosus. The positively charged lysine-rich fifth domain of β2GPI facilitates its interaction with phospholipid membranes containing acidic phospholipids, which normally become exposed by apoptotic processes. In the present study, atomic force microscopy was applied to visualize the binding of β2GPI to a mixed phospholipid model membrane at physiological ionic strength. On supported lipid bilayers the formation of supramolecular assemblies of the protein with a height of approx. 3.3 nm was observed, suggesting a lateral agglomeration of β2GPI. Detailed analysis of kinetic constants using surface plasmon resonance revealed that the binding can be described by a two-state reaction model, i.e. a very fast interaction step, depending on the content of acidic phospholipids in the bilayer, and a second step with significantly lower kon and koff values. Taken together, our results suggest a biphasic interaction mechanism: a fast step of β2GPI binding to negatively charged lipids, mainly based on electrostatic interactions, and a slower phase of agglomeration of the protein on the bilayer surface accompanied by a protein-induced rigidification of the membrane, as revealed by electron paramagnetic resonance.

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