In Situ Precipitation of Amorphous Calcium Phosphate and Ciprofloxacin Crystals during the Formation of Chitosan Hydrogels and Its Application for Drug Delivery Purposes

Langmuir ◽  
2012 ◽  
Vol 28 (45) ◽  
pp. 15937-15946 ◽  
Author(s):  
Stefania Nardecchia ◽  
María C. Gutiérrez ◽  
M. Concepción Serrano ◽  
Mariella Dentini ◽  
Andrea Barbetta ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Chitosan Hydrogels

scholarly journals Effects of calcium phosphates incorporation on structural, thermal and drug-delivery properties of collagen:chitosan scaffolds

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Mirella Romanelli Vicente Bertolo ◽  
Virginia Conceição Amaro Martins ◽  
Ana Maria De Guzzi Plepis
Keyword(s):  
Drug Delivery ◽  
Calcium Phosphate ◽  
Calcium Phosphates ◽  
Great Influence ◽  
Collagen Gel ◽  
Dry Mass ◽  
Ex Situ ◽  
Porous Scaffolds ◽  
Drug Delivery Vehicles

In this study, we evaluated how different procedures of calcium phosphate synthesis and its incorporation in collagen:chitosan scaffolds could affect their structural and thermal properties, aiming the obtention of homogeneous scaffolds which can act as drug delivery vehicles in bone tissue engineering. Therefore, three different scaffold preparation procedures were developed, changing the order of addition of the components: in CC-CNPM1 and CC-CNPM2, calcium phosphate synthesis was performed in situ in the chitosan gel (1%, w/w) followed by mixture with collagen (1%, w/w), with changes in the reagents used for calcium phosphate formation; in CC-CNPM3 procedure, calcium phosphate was synthesized ex situ and then incorporated into the collagen gel, in which chitosan in powder was mixed. In all procedures, 5% (in dry mass) of ciprofloxacin was incorporated. FTIR analysis confirmed the presence of calcium phosphate in all scaffolds. DSC curves showed that collagen denaturation temperature (Td) increased with calcium incorporation. SEM photomicrographs of scaffolds cross-section revealed porous scaffolds with calcium phosphate grains internally distributed in the polymeric matrix. XRD diffractograms indicated that the calcium phosphates obtained are hydroxyapatite. The pore size distribution was more homogeneous for CC-CNPM3, which also stood out for its smaller porosity and lower absorption in PBS. These results indicate that the in situ or ex situ phosphate incorporation in the scaffolds had a great influence on its structural properties, which also had consequences for ciprofloxacin release. CC-CNPM3 released a smaller amount of antibiotic (30%), but its release profile was better described by all the tested models.

scholarly journals Cytotoxicity of amorphous calcium phosphate multifunctional composite coatings on titanium obtained by in situ anodization/anaphoretic deposition

2021 ◽  
pp. 24-24
Author(s):  
Marijana Pantovic-Pavlovic ◽  
Miroslav Pavlovic ◽  
Jovanka Kovacina ◽  
Boris Stanojevic ◽  
Jasmina Stevanovic ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Composite Coatings ◽  
Fibroblast Cell ◽  
Human Lung Fibroblast ◽  
Viable Cells ◽  
Lung Fibroblast Cell Line ◽  
Chitosan Lactate ◽  
Composite Samples

Cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional and hybrid composite coatings on MRC-5 human lung fibroblast cell line is elucidated. ACP/TiO2 and ACP/TiO2/ChOL are deposited onto Ti by novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there was no significant decrease in the survival of healthy MRC-5 cells eposed to composite samples without chitosan lactate, while there was an increase in the number of viable cells in the sample containing ChOL. The findings show that there is improved cell proliferation, differentiation and cell viability in the ChOL-containing sample, which makes ACP/TiO2/ChOL coating a good candidate for the applications in medicine and stomatology.

scholarly journals Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

Nanomaterials ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 20 ◽  
Author(s):  
Rui Sun ◽  
Michelle Åhlén ◽  
Cheuk-Wai Tai ◽  
Éva G. Bajnóczi ◽  
Fenne de Kleijne ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Large Angle ◽  
Drug Carriers ◽  
Amorphous Calcium Carbonate ◽  
Practical Applications ◽  
The Stability ◽  
Highly Porous

Amorphous calcium phosphate (ACP) has shown significant effects on the biomineralization and promising applications in bio-medicine. However, the limited stability and porosity of ACP material restrict its practical applications. A storage stable highly porous ACP with Brunauer–Emmett–Teller surface area of over 400 m2/g was synthesized by introducing phosphoric acid to a methanol suspension containing amorphous calcium carbonate nanoparticles. Electron microscopy revealed that the porous ACP was constructed with aggregated ACP nanoparticles with dimensions of several nanometers. Large angle X-ray scattering revealed a short-range atomic order of <20 Å in the ACP nanoparticles. The synthesized ACP demonstrated long-term stability and did not crystallize even after storage for over 14 months in air. The stability of the ACP in water and an α-MEM cell culture medium were also examined. The stability of ACP could be tuned by adjusting its chemical composition. The ACP synthesized in this work was cytocompatible and acted as drug carriers for the bisphosphonate drug alendronate (AL) in vitro. AL-loaded ACP released ~25% of the loaded AL in the first 22 days. These properties make ACP a promising candidate material for potential application in biomedical fields such as drug delivery and bone healing.

Fluoride and Casein Phosphopeptide-Amorphous Calcium Phosphate

2008 ◽  
Vol 87 (4) ◽  
pp. 344-348 ◽  
Author(s):  
E.C. Reynolds ◽  
F. Cai ◽  
N.J. Cochrane ◽  
P. Shen ◽  
G.D. Walker ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Double Blind ◽  
Water Slurry ◽  
Supragingival Plaque ◽  
Casein Phosphopeptide

Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) slows the progression of caries and remineralizes enamel subsurface lesions. The aim of this study was to determine the ability of CPP-ACP to increase the incorporation of fluoride into plaque and to promote enamel remineralization in situ. Randomized, double-blind, cross-over studies involved mouthrinses and dentifrices containing CPP-ACP and fluoride. The mouthrinses were used for 60 sec, three times/day for 5 days, and supragingival plaque was collected and analyzed for F. The dentifrices were rinsed as a water slurry for 60 sec four times/day for 14 days in an in situ model. The addition of 2% CPP-ACP to the 450-ppm-F mouthrinse significantly increased the incorporation of fluoride into plaque. The dentifrice containing 2% CPP-ACP produced a level of remineralization similar to that achieved with a dentifrice containing 2800 ppm F. The dentifrice containing 2% CPP-ACP plus 1100 ppm F was superior to all other formulations.

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