Cationic Detergent/Sterol Mixtures Can Form Fluid Lamellar Phases and Stable Unilamellar Vesicles

Langmuir ◽  
2009 ◽  
Vol 25 (10) ◽  
pp. 5778-5784 ◽  
Author(s):  
Thida Phoeung ◽  
Liliana Morfin Huber ◽  
Michel Lafleur
Keyword(s):  
Unilamellar Vesicles ◽  
Cationic Detergent ◽  
Lamellar Phases

Orientation of lamellar phases of lyotropic multicomponent mixtures, based on cetyltrimethylammonium bromide cationic detergent, in magnetic field

2013 ◽  
Vol 58 (6) ◽  
pp. 902-905
Author(s):  
E. O. Kiirend ◽  
S. P. Chumakova ◽  
T. I. Pekhk ◽  
N. R. Ivanov
Keyword(s):  
Magnetic Field ◽  
Cetyltrimethylammonium Bromide ◽  
Multicomponent Mixtures ◽  
Cationic Detergent ◽  
Lamellar Phases

Undulation-enhanced electrostatic forces in lamellar phases of fluid membranes

1994 ◽  
Vol 4 (9) ◽  
pp. 1541-1555 ◽  
Author(s):  
Renko de Vries
Keyword(s):  
Electrostatic Forces ◽  
Lamellar Phases ◽  
Fluid Membranes

Application of RT-PCR for the Detection of Enteric Viruses in Oysters

1993 ◽  
Vol 27 (3-4) ◽  
pp. 49-53 ◽  
Author(s):  
L. A. Jaykus ◽  
R. De Leon ◽  
M. D. Sobsey
Keyword(s):  
Enteric Viruses ◽  
Rt Pcr ◽  
Chloroform Extraction ◽  
Cationic Detergent

Oyster samples processed by adsorption-elution-precipitation were seeded with poliovirus 1 and HAV, and cleaned and concentrated by Freon extraction (2X), PEG precipitation and chloroform extraction. Freon extraction resulted in recoveries of 63-76% for polio and 42-52% for HAV. PEG precipitation/chloroform extraction gave recoveries of 47-50% for polio and 15-19% for HAV. Treated extracts inhibited RT-PCR at 10−2 dilutions. Inhibitors were removed by treatment with the cationic detergent CTAB or Pro-Cipitate/UF adsorption-elution-concentration. Both treatments resulted in samples on which direct RT-PCR was possible. The CTAB procedure was able to detect 78 pfu of polio and 295 pfu of HAV. The Pro-Cipitate procedure was able to detect 70 pfu polio and 2.1×103 pfu HAV.

scholarly journals Highly Stable Phospholipid Unilamellar Vesicles from Spontaneous Vesiculation:  A DLS and SANS Study

2005 ◽  
Vol 109 (1) ◽  
pp. 609-616 ◽  
Author(s):  
Baohua Yue ◽  
Chien-Yueh Huang ◽  
Mu-Ping Nieh ◽  
Charles J. Glinka ◽  
John Katsaras
Keyword(s):  
Unilamellar Vesicles

scholarly journals Double-Chain Cationic Surfactants: Swelling, Structure, Phase Transitions and Additive Effects

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3946
Author(s):  
Rui A. Gonçalves ◽  
Yeng-Ming Lam ◽  
Björn Lindman
Keyword(s):  
Phase Transitions ◽  
Phase Behaviour ◽  
Double Chain ◽  
Scanning Calorimetry ◽  
Alkyl Chains ◽  
X Ray Scattering ◽  
Amphiphilic Compounds ◽  
Liquid Phases ◽  
Lamellar Phases ◽  
Water Layers

Double-chain amphiphilic compounds, including surfactants and lipids, have broad significance in applications like personal care and biology. A study on the phase structures and their transitions focusing on dioctadecyldimethylammonium chloride (DODAC), used inter alia in hair conditioners, is presented. The phase behaviour is dominated by two bilayer lamellar phases, Lβ and Lα, with “solid” and “melted” alkyl chains, respectively. In particular, the study is focused on the effect of additives of different polarity on the phase transitions and structures. The main techniques used for investigation were differential scanning calorimetry (DSC) and small- and wide-angle X-ray scattering (SAXS and WAXS). From the WAXS reflections, the distance between the alkyl chains in the bilayers was obtained, and from SAXS, the thicknesses of the surfactant and water layers. The Lα phase was found to have a bilayer structure, generally found for most surfactants; a Lβ phase made up of bilayers with considerable chain tilting and interdigitation was also identified. Depending mainly on the polarity of the additives, their effects on the phase stabilities and structure vary. Compounds like urea have no significant effect, while fatty acids and fatty alcohols have significant effects, but which are quite different depending on the nonpolar part. In most cases, Lβ and Lα phases exist over wide composition ranges; certain additives induce transitions to other phases, which include cubic, reversed hexagonal liquid crystals and bicontinuous liquid phases. For a system containing additives, which induce a significant lowering of the Lβ–Lα transition, we identified the possibility of a triggered phase transition via dilution with water.

scholarly journals A Study on the Damping Capacities of Mg–Zn–Y-Based Alloys with Lamellar Long Period Stacking Ordered Phases by Preparation Process

Metals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
Ruopeng Lu ◽  
Kai Jiao ◽  
Yuhong Zhao ◽  
Kun Li ◽  
Keyu Yao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Solid Solution ◽  
Damping Capacity ◽  
Second Phase ◽  
Preparation Process ◽  
Obvious Effect ◽  
Lpso Phase ◽  
Long Period ◽  
Lamellar Phases ◽  
Ordered Phases

Mg alloys with fine mechanical properties and high damping capacities are essential in engineering applications. In this work, Mg–Zn–Y based alloys with lamellar long period stacking ordered (LPSO) phases were obtained by different processes. The results show that a more lamellar second phase can be obtained in the samples with more solid solution atoms. The density of the lamellar LPSO phase has an obvious effect on the damping of the magnesium alloy. The compact LPSO phase is not conducive to dislocation damping, but sparse lamellar phases can improve the damping capacity without significantly reducing the mechanical properties. The Mg95.3Zn2Y2.7 alloy with lamellar LPSO phases and ~100 μm grain size exhibited a fine damping property of 0.110 at ε = 10–3.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

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