Modulation of Emission and Singlet Oxygen Photosensitization in Live Cells Utilizing Bioorthogonal Phosphorogenic Probes and Protein Tag Technology

2021 ◽  
Author(s):  
Peter Kam-Keung Leung ◽  
Lawrence Cho-Cheung Lee
Keyword(s):  
Singlet Oxygen ◽  
Live Cells ◽  
Protein Tag

Modulation of emission and singlet oxygen photosensitisation in live cells utilising bioorthogonal phosphorogenic probes and protein tag technology

2020 ◽  
Vol 56 (45) ◽  
pp. 6074-6077 ◽  
Author(s):  
Peter Kam-Keung Leung ◽  
Kenneth Kam-Wing Lo
Keyword(s):  
Singlet Oxygen ◽  
Live Cells ◽  
Protein Tag ◽  
Single Oxygen

We developed a strategy to exploit the bioorthogonal reactivity and phosphorogenic property of iridium(iii) polypyridine nitrone complexes and SNAP-tag protein for the modulation of emission and single oxygen photosensitisation in live cells.

Hybrids of a 9-anthracenyl moiety and fluorescein as chemodosimeters for the detection of singlet oxygen in live cells

2019 ◽  
Vol 17 (46) ◽  
pp. 9883-9891 ◽  
Author(s):  
Serghei Chercheja ◽  
Steffen Daum ◽  
Hong-Gui Xu ◽  
Frank Beierlein ◽  
Andriy Mokhir
Keyword(s):  
Singlet Oxygen ◽  
Live Cells ◽  
Highly Efficient

A highly efficient fluorogenic chemodosimeter for the detection of singlet oxygen was developed.

scholarly journals Design of a protein tag and fluorogenic probe with modular structure for live-cell imaging of intracellular proteins

2016 ◽  
Vol 7 (1) ◽  
pp. 308-314 ◽  
Author(s):  
Yuko Kamikawa ◽  
Yuichiro Hori ◽  
Kazuo Yamashita ◽  
Lin Jin ◽  
Shinya Hirayama ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Modular Structure ◽  
Live Cells ◽  
Fluorogenic Probe ◽  
Intracellular Proteins ◽  
Protein Tag ◽  
Modular Structures

Quick and no-wash labeling of intracellular proteins was achieved in live cells using a PYP-tag mutant and a membrane-permeable fluorogenic probe with modular structures.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

Fluoroquinolone Antimicrobials: Singlet Oxygen, Superoxide and Phototoxicity

1998 ◽  
Vol 67 (4) ◽  
pp. 399 ◽  
Author(s):  
Lydia J. Martínez ◽  
Robert H. Sik ◽  
Colin F. Chignell
Keyword(s):  
Singlet Oxygen
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