4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

scholarly journals Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (8) ◽  
Author(s):  
Jan Huebinger ◽  
Martin Masip ◽  
Jens Christmann ◽  
Frank Wehner ◽  
Philippe Bastiaens
Keyword(s):  
High Resolution ◽  
Living Cells ◽  
High Resolution Imaging ◽  
Resolution Imaging

High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein

2008 ◽  
Vol 1 (43) ◽  
pp. pl3-pl3 ◽  
Author(s):  
G. Maulucci ◽  
V. Labate ◽  
M. Mele ◽  
E. Panieri ◽  
G. Arcovito ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Redox Signaling ◽  
Live Cells ◽  
High Resolution Imaging ◽  
Resolution Imaging

Detecting and imaging of SO2 derivatives in living cells with zero cross-talk colorimetric mitochondria-targeted fluorescent probe

2020 ◽  
Vol 98 (1) ◽  
pp. 1-6
Author(s):  
Dan Wu ◽  
Shiqi Rong ◽  
Yi Liu ◽  
Fei Zheng ◽  
Yankun Zhao ◽  
...  
Keyword(s):  
High Resolution ◽  
Cross Talk ◽  
High Sensitivity ◽  
Living Cells ◽  
High Resolution Imaging ◽  
Biochemical Processes ◽  
Highly Sensitive ◽  
Resolution Imaging ◽  
Low Cytotoxicity

It is well known that excessive levels of sulfur dioxide and its derivatives are connected to diverse diseases. Therefore, developing highly sensitive probes to detect and monitor sulfite in living cells is important for the diagnosis of disease and the study of biochemical processes in vivo. In this report, two zero cross-talk ratiometric fluorescent probes were synthesized (CA-ID-MC and CA-BI-MC), which were derived from carbazole-indolenine π-conjugated system for effective detection of sulfite in living cells. Observably, CA-BI-MC exhibited the largest emission shift of 157 nm from 617 to 460 nm with the addition of various concentrations of sulfite, which is beneficial for high-resolution imaging of the sulfite. CA-BI-MC also exhibits high sensitivity and low cytotoxicity. More importantly, this probe successfully located mitochondria and sensed the sulfite in HeLa cells caused by exogenous stimulation.

A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells

2019 ◽  
Vol 17 (15) ◽  
pp. 3732-3736 ◽  
Author(s):  
Adiki Raja Sekhar ◽  
Bhagaban Mallik ◽  
Vimlesh Kumar ◽  
Jeyaraman Sankar
Keyword(s):  
Endoplasmic Reticulum ◽  
High Resolution ◽  
Small Molecule ◽  
Super Resolution ◽  
Live Cells ◽  
High Resolution Imaging ◽  
A Cell ◽  
Resolution Imaging

A simple BODIPY-based small molecule has been identified to selectively label the endoplasmic reticulum for high-resolution imaging with negligible cytotoxicity.

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