H-ras Dependent Estrogenic Effects of Epidermal Growth Factor in the Estrogen-Independent Breast Cancer Cell Line MDA-MB-231

2003 ◽  
Vol 80 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Oliver Treeck ◽  
Angela Weber ◽  
Michaela Boester ◽  
Simone Porz ◽  
Nicola Frey ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Estrogenic Effects ◽  
Epidermal Growth

scholarly journals Insulin-Like Growth Factor-1 Signaling Regulates miRNA Expression in MCF-7 Breast Cancer Cell Line

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49067 ◽  
Author(s):  
Elizabeth C. Martin ◽  
Melyssa R. Bratton ◽  
Yun Zhu ◽  
Lyndsay V. Rhodes ◽  
Syreeta L. Tilghman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Mirna Expression ◽  
Cancer Cell Line ◽  
Insulin Like Growth Factor ◽  
Mcf 7

scholarly journals Epidermal growth factor receptor gene-amplified MDA-468 breast cancer cell line and its nonamplified variants.

1987 ◽  
Vol 7 (1) ◽  
pp. 251-257 ◽  
Author(s):  
J Filmus ◽  
J M Trent ◽  
M N Pollak ◽  
R N Buick
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Egf Receptor ◽  
Receptor Gene ◽  
Cancer Cell Line ◽  
Egf Receptors ◽  
Epidermal Growth

We have recently reported (J. Filmus, M. N. Pollak, R. Cailleau, and R. N. Buick, Biochem. Biophys. Res. Commun. 128:898-905, 1985) that MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited in vitro pharmacological doses of EGF. We have derived several MDA-468 clonal variants which are resistant to EGF-induced growth inhibition. These clones had a number of EGF receptors, similar to normal human fibroblasts, and had lost the EGF receptor gene amplification. Karyotype analysis showed that MDA-468 cells had an abnormally banded region (ABR) in chromosome 7p which was not present in the variants. It was shown by in situ hybridization that the amplified EGF receptor sequences were located in that chromosome, 7pABR. Five of the six variants studied were able to generate tumors in nude mice, but their growth rate was significantly lower than that of tumors derived from the parental cell line. The variant that was unable to produce tumors was found to be uniquely dependent on EGF for growth in soft agar.

scholarly journals Epidermal growth factor receptor gene-amplified MDA-468 breast cancer cell line and its nonamplified variants

1987 ◽  
Vol 7 (1) ◽  
pp. 251-257
Author(s):  
J Filmus ◽  
J M Trent ◽  
M N Pollak ◽  
R N Buick
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Breast Cancer Cell Line ◽  
Egf Receptor ◽  
Receptor Gene ◽  
Cancer Cell Line ◽  
Egf Receptors ◽  
Epidermal Growth

We have recently reported (J. Filmus, M. N. Pollak, R. Cailleau, and R. N. Buick, Biochem. Biophys. Res. Commun. 128:898-905, 1985) that MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited in vitro pharmacological doses of EGF. We have derived several MDA-468 clonal variants which are resistant to EGF-induced growth inhibition. These clones had a number of EGF receptors, similar to normal human fibroblasts, and had lost the EGF receptor gene amplification. Karyotype analysis showed that MDA-468 cells had an abnormally banded region (ABR) in chromosome 7p which was not present in the variants. It was shown by in situ hybridization that the amplified EGF receptor sequences were located in that chromosome, 7pABR. Five of the six variants studied were able to generate tumors in nude mice, but their growth rate was significantly lower than that of tumors derived from the parental cell line. The variant that was unable to produce tumors was found to be uniquely dependent on EGF for growth in soft agar.

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