scholarly journals Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium

2003 ◽  
Vol 25 (24) ◽  
pp. 2089-2092 ◽  
Author(s):  
C.C. Pacheco ◽  
C.C. Alves ◽  
L. Barreiros ◽  
P.M.L. Castro ◽  
P.C.M. Teixeira
Keyword(s):  
Bacterial Enumeration ◽  
Epifluorescence Microscope

scholarly journals Microbial Growth Study on Pork Loins as Influenced by the Application of Different Antimicrobials

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 968
Author(s):  
David A. Vargas ◽  
Markus F. Miller ◽  
Dale R. Woerner ◽  
Alejandro Echeverry
Keyword(s):  
Storage Time ◽  
Microbial Growth ◽  
Storage Conditions ◽  
Indicator Bacteria ◽  
Aerobic Bacteria ◽  
Growth Study ◽  
Treatment Interventions ◽  
Pork Industry ◽  
Bacterial Enumeration ◽  
The Impact

The use of antimicrobials in the pork industry is critical in order to ensure food safety and, at the same time, extend shelf life. The objective of the study was to determine the impact of antimicrobials on indicator bacteria on pork loins under long, dark, refrigerated storage conditions. Fresh boneless pork loins (n = 36) were split in five sections and treated with antimicrobials: Water (WAT), Bovibrom 225 ppm (BB225), Bovibrom 500 ppm (BB500), Fit Fresh 3 ppm (FF3), or Washing Solution 750 ppm (WS750). Sections were stored for 1, 14, 28, and 42 days at 2–4 °C. Mesophilic and psychrotrophic aerobic bacteria (APC-M, APC-P), lactic acid bacteria (LAB-M), coliforms, and Escherichia coli were enumerated before intervention, after intervention, and at each storage time. All bacterial enumeration data were converted into log10 for statistical analysis, and the Kruskal–Wallis test was used to find statistical differences (p < 0.05). Initial counts did not differ between treatments, while, after treatment interventions, treatment WS750 did not effectively reduce counts for APC-M, APC-P, and coliforms (p < 0.01). BB500, FF3, and WS750 performed better at inhibiting the growth of indicator bacteria when compared with water until 14 days of dark storage.

Assessment of Physicochemical Characteristics and Thermophilic Bacterial Enumeration from Hot Water Springs of Himachal Pradesh

2018 ◽  
Vol 41 (5) ◽  
pp. 285-287
Author(s):  
Poonam Shirkot ◽  
Ambika Verma ◽  
Monika Gupta ◽  
Anshul Sharma Manjul ◽  
Ruchika Sharma
Keyword(s):  
Physicochemical Characteristics ◽  
Himachal Pradesh ◽  
Hot Water ◽  
Bacterial Enumeration ◽  
Water Springs

scholarly journals Flat-top illumination profile in an epifluorescence microscope by dual microlens arrays

2012 ◽  
Vol 81A (4) ◽  
pp. 324-331 ◽  
Author(s):  
Frank A. W. Coumans ◽  
Edwin van der Pol ◽  
Leon W. M. M. Terstappen
Keyword(s):  
Microlens Arrays ◽  
Epifluorescence Microscope

scholarly journals Evaluation of bacteriological parameters and heavy metal contents on water of Tinau river, Butwal, Rupandehi, Nepal

Our Nature ◽  
2017 ◽  
Vol 15 (1-2) ◽  
pp. 1-6
Author(s):  
Basant Pandey ◽  
Anjana Devkota
Keyword(s):  
Heavy Metal ◽  
Surface Water Quality ◽  
Heavy Metal Concentration ◽  
Total Coliform ◽  
Flame Atomic Absorption ◽  
Metal Contents ◽  
Bacterial Enumeration ◽  
Different Seasons ◽  
Plate Counts ◽  
Bacteriological Parameters

The present work was undertaken to assess the surface water quality and discuss the potability of water by analyzed data of bacteriological (total Coliform and Faecalform) characters and heavy metals content (Fe, Mn, Zn, Pb, Cr and Cu) in the water of Tinau river. The study was carried out in the year 2011/2012 by selecting four spots within three different seasons: winter, summer and rainy. For bacterial enumeration, bacterial plate counts were carried out using the pour plate method with nutrient agar; while for determination of heavy metal concentration, Flame Atomic Absorption Spectro-photometry was used. The heavy metal content was within the range of WHO guideline for drinking purpose. However, on the basis of bacteriological parameters the water was not safe to consume without treatment. This study will also help to make aware those local people or adjacent farmers for proper management of waste disposal and also to minimize use of synthetic inputs.

Microbiological, Sensory, and Electronic Nose Evaluation of Yellowfin Tuna under Various Storage Conditions†

2001 ◽  
Vol 64 (12) ◽  
pp. 2027-2036 ◽  
Author(s):  
WEN-XIAN DU ◽  
JEONGMOK KIM ◽  
JOHN A. CORNELL ◽  
TUNG-SHI HUANG ◽  
MAURICE R. MARSHALL ◽  
...  
Keyword(s):  
Electronic Nose ◽  
Bacterial Load ◽  
Storage Conditions ◽  
Yellowfin Tuna ◽  
Plate Count ◽  
Sensory Panel ◽  
Objective Indicator ◽  
Microbial Counts ◽  
Plate Count Agar ◽  
Bacterial Enumeration

Microbiological assessment, sensory evaluation, and electronic nose (AromaScan) analysis were performed on yellowfin tuna stored at 0, 4, 10, and 22°C for 0, 1, 3, 5, and 9 days. Fish color, texture, appearance, and odor were evaluated by a trained sensory panel, while aroma-odor properties were evaluated using an AromaScan. Bacterial enumeration was performed using plate count agar containing 1.5% NaCl. Tuna fillets stored at 22°C for 3 days or longer had a bacterial load of over 107 CFU/g and were rated not acceptable for consumption (grade C) by the sensory panel. Tuna fillets stored at 4°C for 9 days or 10°C for over 5 days were rated as grade C products and also had a bacterial load of over 107 CFU/g. The change in fish quality as determined by AromaScan followed increases in microbiological counts in tuna fillets, indicating that bacterial load can serve as a useful and objective indicator of gross spoilage. Electronic nose devices can be used in conjunction with microbial counts and sensory panels to evaluate the degree of decomposition in tuna during storage.

Molecular bacterial diversity and bioburden of commercial airliner cabin air

2007 ◽  
Vol 53 (11) ◽  
pp. 1259-1271 ◽  
Author(s):  
Myron T. La Duc ◽  
Tara Stuecker ◽  
Kasthuri Venkateswaran
Keyword(s):  
Bacterial Diversity ◽  
Rrna Gene ◽  
Human Respiratory Tract ◽  
Atp Assay ◽  
Gram Positive Bacteria ◽  
Air Impingement ◽  
Culture Independent ◽  
Bacterial Enumeration ◽  
Gradual Accumulation ◽  
Opportunistic Pathogenic

Culture-independent, biomarker-targeted bacterial enumeration and identification strategies were employed to estimate total bacterial burden and diversity within the cabin air of commercial airliners. Samples from each of 4 flights on 2 commercial carriers were collected via air-impingement. The total viable microbial population ranged from below detection limits to 4.1 × 106cells/m3of air, as assessed by the ATP assay. A gradual accumulation of microbes was observed from the time of passenger boarding through mid-flight, followed by a sharp decline in bacterial abundance and viability from the initiation of descent through landing. Representatives of the α-, β-, and γ-Proteobacteria, as well as Gram-positive bacteria, were isolated in varying abundance. Neisseria meningitidis rRNA gene sequences were retrieved in great abundance from Airline A followed by Streptococcus oralis/mitis sequences. Pseudomonas synxantha sequences dominated Airline B clone libraries, followed by those of N. meningitidis and S. oralis/mitis. The cabin air samples examined herein housed low bacterial diversity and were often dominated by a particular subset of bacteria: opportunistic pathogenic inhabitants of the human respiratory tract and oral cavity.

scholarly journals Effects of Dietary Fat Source and Subtherapeutic Levels of Antibiotic on the Bacterial Community in the Ileum of Broiler Chickens at Various Ages

2002 ◽  
Vol 68 (12) ◽  
pp. 5918-5924 ◽  
Author(s):  
Ane Knarreborg ◽  
Mary Alice Simon ◽  
Ricarda M. Engberg ◽  
Bent Borg Jensen ◽  
Gerald W. Tannock
Keyword(s):  
Bacterial Community ◽  
Dietary Fat ◽  
Broiler Chickens ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Dietary Supplementation ◽  
Toxin Gene ◽  
Bacterial Enumeration ◽  
Gradient Gel Electrophoresis ◽  
Different Strains ◽  
Dietary Treatments

ABSTRACT The effect of dietary fat source (soy oil or a mixture of lard and tallow) and dietary supplementation with antibiotics (a combination of avilamycin at 10 mg kg of feed−1 and salinomycin at 40 mg kg of feed−1) on the bacterial community in the ileum of broiler chickens at different ages (7, 14, 21, and 35 days) was studied using PCR with denaturing gradient gel electrophoresis (DGGE) analysis and bacteriological culture. The bacterial origin of fragments in DGGE profiles was identified by sequencing. Bacterial enumeration results, together with PCR-DGGE profiles, showed that the composition of the microflora was age dependent and influenced by dietary fat source and antibiotic supplementation. An increased incidence of streptococci, enterobacteria, and Clostridium perfringens with age of the chickens was demonstrated. Lactobacilli and C. perfringens were the bacterial groups most strongly affected by the dietary treatments. Moreover, different strains (clonal variants of the alpha-toxin gene) of C. perfringens type A were detected in response to age, dietary fat source, and dietary supplementation with antibiotics.

Real-Time Nucleic Acid–Based Detection Methods for Pathogenic Bacteria in Food

2004 ◽  
Vol 67 (4) ◽  
pp. 823-832 ◽  
Author(s):  
JOHN L. McKILLIP ◽  
MARYANNE DRAKE
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Pathogen Detection ◽  
Food Microbiology ◽  
Detection Methods ◽  
Target Sequence ◽  
Bacterial Enumeration

Quality assurance in the food industry in recent years has involved the acceptance and implementation of a variety of nucleic acid–based methods for rapid and sensitive detection of food-associated pathogenic bacteria. Techniques such as polymerase chain reaction have greatly expedited the process of pathogen detection and have in some cases replaced traditional methods for bacterial enumeration in food. Conventional PCR, albeit sensitive and specific under optimized conditions, obligates the user to employ agarose gel electrophoresis as the means for endpoint analysis following sample processing. For the last few years, a variety of real-time PCR chemistries and detection instruments have appeared on the market, and many of these lend themselves to applications in food microbiology. These approaches afford a user the ability to amplify DNA or RNA, as well as detect and confirm target sequence identity in a closed-tube format with the use of a variety of fluorophores, labeled probes, or both, without the need to run gels. Such real-time chemistries also offer greater sensitivity than traditional gel visualization and can be semiquantitative and multiplexed depending on the specific experimental objectives. This review emphasizes the current systems available for real-time PCR–based pathogen detection, the basic mechanisms and requirements for each, and the prospects for development over the next few years in the food industry.

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