Lycopene hampers lung injury due to skeletal muscle ischemia-reperfusion in rat model

2020 ◽  
pp. 1-8
Author(s):  
Mehmet Kirisci ◽  
Bulent Guneri ◽  
Muhammed Seyithanoglu ◽  
Ulku Kazanci
Keyword(s):  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Lung Injury ◽  
Ischemia Reperfusion ◽  
Serum Levels ◽  
Control Group ◽  
Muscle Ischemia ◽  
Group I ◽  
Preventive Efficacy ◽  
The Mean

Abstract. This study investigates lycopene’s preventive efficacy in skeletal muscle ischemia-reperfusion (I/R) induced lung injury. Thirty-two rats were randomly assigned to control group, lycopene group, I/R group and I/R + lycopene group. In the lycopene and I/R + lycopene groups, the rats initially received 10 mg/kg/day lycopene orally for 15 days. Then, dissection around the abdominal aorta was performed in all rats under general anesthesia. The aorta was clamped at the infrarenal level in the I/R group and I/R + lycopene group for two hours before two hours of reperfusion. The mean serum levels of malondialdehyde (53.0 ± 20.14 nmol/mL) and superoxide dismutase (1.03 ± 0.16 U/mL) were higher and lower in the I/R group than the other three groups, respectively (p < 0.001). The mean serum IMA level of I/R + lycopene group (0.42 ± 0.04 abs/u) was lower than the I/R group (0.47 ± 0.04 abs/u) (p = 0.015). The mean tissue malondialdehyde levels of I/R group (69.10 ± 11.55 nmol/mL) and I/R + lycopene group (68.36 ± 21.17 nmol/mL) were high compared to the control group (49.87 ± 6.52 nmol/mL) and lycopene group (47.82 ± 4.44 nmol/mL) (p = 0.002). The mean tissue glutathione peroxidase (p < 0.001) and superoxide dismutase (p = 0.001) levels of I/R group (121.81 ± 43.59 nmol/mL and 25.17 ± 8.69 U/mL) were low compared to the control group (236.12 ± 18.01 nmol/mL and 46.30 ± 5.17 U/mL), lycopene group (227.52 ± 16.92 nmol/mL and 45.82 ± 4.02 U/mL), and I/R + lycopene group (176.02 ± 24.27 nmol/mL and 35.20 ± 4.85 U/mL). The histopathological analyses of I/R + lycopene group indicated less significant changes than the control group. Tissue damage in the I/R + lycopene group was less prominent than the I/R group. These findings suggest oral lycopene supplementation as a promising prevention against skeletal muscle I/R caused lung injury.

scholarly journals Effect of N-acetylcysteine on lung injury induced by skeletal muscle ischemia-reperfusion: histopathological study in rat model

2012 ◽  
Vol 27 (2) ◽  
pp. 168-171 ◽  
Author(s):  
Amir Sotoudeh ◽  
Mohammad Ashrafzadeh Takhtfooladi ◽  
Amirali Jahanshahi ◽  
Adel Haghighi Khiabanian Asl ◽  
Hamed Ashrafzadeh Takhtfooladi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lung Injury ◽  
Femoral Artery ◽  
Ischemia Reperfusion ◽  
Left Lung ◽  
Male Rats ◽  
Histopathological Analysis ◽  
Muscle Ischemia ◽  
Group I ◽  
Significant Difference

PURPOSE: To investigate whether N-acetylcysteine, a free radicals scavenger has a protective effect against lung injury as a remote organ after skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion + N-acetylcysteine (group II). All animals were undergone two hours of ischemia by occlusion femoral artery and 24h of reperfusion. Before clamped the femoral artery, 250 IU heparin was administered via the jugular vein to prevent clotting. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mgkg-¹, immediately before reperfusion. After 24h of reperfusion, animals were euthanized and left lung harvested for histopathological analysis under light microscopy. RESULTS: In the group I, tissues showed histological changes with intra-alveolar edema, intra-alveolar hemorrhage and neutrophilic infiltration. Histopathologically, there was a significant difference (P = 0.005) between two groups. CONCLUSION: Administration of N-acetylcysteine treatment significantly decreased lung injury induced by skeletal muscle ischemia reperfusion according to histological findings.

scholarly journals The effects of iloprost on lung injury induced by skeletal muscle ischemia–reperfusion

2014 ◽  
Vol 115 (07) ◽  
pp. 405-410 ◽  
Author(s):  
D. Erer ◽  
A. D. Dursun ◽  
G. L. Oktar ◽  
E. Iriz ◽  
M. H. Zor ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lung Injury ◽  
Ischemia Reperfusion ◽  
Muscle Ischemia

scholarly journals Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury

2018 ◽  
Vol 8 (2) ◽  
pp. 91-97 ◽  
Author(s):  
Hassan Ahmadvand ◽  
Esmaeel Babaeenezhad ◽  
Maryam Nasri ◽  
Leila Jafaripour ◽  
Reza Mohammadrezaei Khorramabadi
Keyword(s):  
Oxidative Stress ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Serum Levels ◽  
Renal Ischemia ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Control Group ◽  
Group I ◽  
Liver Markers

Introduction: Glutathione (GSH) protects the tissue and cell from oxidative injury. Objectives: In the current study, we investigated the possible effects of GSH on liver markers, oxidative stress and inflammatory indices in rat with renal ischemia reperfusion (RIR) injury. Materials and Methods: Twenty-four adult male Wistar rats were divided into 3 groups (n=8). Group I (the control group), group II (the RIR group) received saline (0.25 mL/d, intraperitoneally; i.p.), group III as the RIR group that received GSH (100 mg/kg/d, i.p.). The treatment with saline or GSH began daily 14 days before RIR induction. RIR was induced by clamping renal pedicles for 45 minutes and 24 hours of reperfusion. Results: RIR significantly increased the serum level of nitric oxide (NO), the serum activities of aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), the serum and renal levels of malondialdehyde (MDA), and the serum activity of myeloperoxidase (MPO). However, RIR significantly decreased the serum and renal levels of GSH, serum paraoxonase 1 (PON1) activity, and the serum and renal activities of catalase (CAT) and glutathione peroxidase (GPX). GSH administration could significantly improve the serum activities of AST, GGT, MPO, GPX and PON1 and serum levels of NO, renal MDA, GSH levels, and serum and also renal CAT activities. Conclusion: Our study indicated that GSH administration ameliorated RIR injury in rats by improving the activities of liver markers and antioxidant enzymes, the levels of MDA, NO, GSH and MPO activity.

scholarly journals Protective effect of N-acetylcysteine on kidney as a remote organ after skeletal muscle ischemia-reperfusion

2012 ◽  
Vol 27 (9) ◽  
pp. 611-615 ◽  
Author(s):  
Mohammad Ashrafzadeh Takhtfooladi ◽  
Amirali Jahanshahi ◽  
Gholamreza Jahanshahi ◽  
Amir Sotoudeh ◽  
Hamed Ashrafzadeh Takhtfooladi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protective Effect ◽  
Femoral Artery ◽  
Renal Injury ◽  
Ischemia Reperfusion ◽  
Male Rats ◽  
Muscle Ischemia ◽  
Group I ◽  
Remote Organ ◽  
Group Ii

PURPOSE: To investigate whether N-acetylcysteine has a protective effect against renal injury as a remote organ after skeletal muscle ischemia-reperfusion in rats. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion + N-acetylcysteine (group II). After ketamine and xylazine anesthesia, femoral artery was exposed. All animals were undergone 2h of ischemia by occlusion femoral artery and 24h of reperfusion. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mg/kg-¹, immediately before reperfusion. After 24h of reperfusion, the blood samples were collected and submitted for evaluation of plasmatic urea, creatinine values and then rats were euthanized and left kidney harvested for histopathological analysis under light microscopy. RESULTS: The urea (35±7.84 mg.dL-1), creatinine (1.46±0.47 mg.dL-1) values were significantly lower in group II (P=0.000). Renal histopathologic study in group I showed extensive distal and proximal tubular cells necrosis and sloughing of epithelial cells into the tubular lumen, cast formation in tubule and glomerul, glomerul fibrosis and hemorrhage. Histopathologically, there was a significant difference (p=0.037) between two groups. CONCLUSION: The N-acetylcysteine was able to decrease renal injury induced by skeletal muscle ischemia reperfusion in rats.

COX-2 inhibition attenuates lung injury induced by skeletal muscle ischemia reperfusion in rats

2016 ◽  
Vol 31 ◽  
pp. 116-122 ◽  
Author(s):  
Liangrong Wang ◽  
Yuanlu Shan ◽  
Yuzhu Ye ◽  
Lida Jin ◽  
Qian Zhuo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lung Injury ◽  
Ischemia Reperfusion ◽  
Muscle Ischemia ◽  
Cox 2

scholarly journals Preserved Ovarian Function Following Toxicity With Doxorubicin in Rats: Protective Effect of Nasturtium Officinale Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 57-64
Author(s):  
Parastou Rad ◽  
◽  
Fahimeh Safari ◽  
Jamshid Mohammadi ◽  
Hamdollah Delaviz ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Serum Levels ◽  
Saline Group ◽  
Female Rats ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Nasturtium Officinale ◽  
Group I ◽  
The Mean

Background: Chemotherapy agents can cause ovarian dysfunction and eventually lead to infertility. This study investigated the effect of nasturtium officinale extract on the ovarian function following the toxicity induced by doxorubicin in female rats. Methods: Forty eight female Wistar rats (180-210g) were randomly divided in six groups as follows: Group I, normal rats receiving 1ml normal saline; Group II and III receiving 25 and 75 mg/kg of the extract daily by gavage for 21 days. Groups IV, V and VI receiving 10 mg/kg doxorubicin intraperitoneally on the first day. In addition, Group IV and V received 25 and 75 mg/kg of the extract, respectively. The serum levels of estrogen, progesterone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and ovarian Malondialdehyde (MDA) were determined after 21 days of treatment. The mean numbers of various graafian follicles and corpus lutea were recorded after treatment. Results: The mean serum FSH level in Group VI (0.11±0.01) significantly reduced compared to those in Groups II (0.21±0.05) and III (0.23±0.01), (P<0.05). The mean serum LH and estrogen levels in Group VI (0.16±0.08) reduced insignificantly compared to those in the controls (0.21±0.02), and in Groups II (0.23±0.03) and III (0.22±0.09). A significant reduction in the number of primary, secondary and graafian follicles were observed in Group VI compared to the control group (P<0.05). The serum MDA level significantly declined in Group V compared to that in Group VI (P<0.05). Conclusion: The nasturtium officinale extract attenuated the toxic effect of doxorubicin on the rat ovaries and protected the cell division in the follicles and the oocytes maturation.

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