scholarly journals Fibroblast growth in the soft agar clonogenic assay for cervix cancer radiosensitivity

1998 ◽  
Vol 78 (4) ◽  
pp. 550-551 ◽  
Author(s):  
B Stausbøl-Grøn ◽  
H Havsteen ◽  
J Overgaard
Keyword(s):  
Clonogenic Assay ◽  
Soft Agar ◽  
Cervix Cancer ◽  
Fibroblast Growth ◽  
Soft Agar Clonogenic Assay

scholarly journals Three New Steroid Biglycosides, Plancisides A, B, and C, from the Starfish Acanthaster planci

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Alla A. Kicha ◽  
Thi H. Dinh ◽  
Natalia V. Ivanchina ◽  
Timofey V. Malyarenko ◽  
Anatoly I. Kalinovsky ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Colony Formation ◽  
Clonogenic Assay ◽  
Ethanolic Extract ◽  
Soft Agar ◽  
Steroid Glycoside ◽  
Acanthaster Planci ◽  
Hct 116 ◽  
Soft Agar Clonogenic Assay

Three new steroid biglycosides, plancisides A-C (1-3), were isolated from the ethanolic extract of the starfish Acanthaster planci. The structures of 1-3 were determined by extensive NMR and ESI-MS techniques, as (24 S)-28- O-[β-D-galactofuranosyl-(1→5)-α-L-arabinofuranosyl]-24-methyl-5α-cholestane-3β,4β,6α,8,15β,16β,28-heptol (1), (24 S)-28- O-[α-L-fucopyranosyl-(1→2)-3- O-methyl-β-D-xylopyranosyl]–24-methyl-5α-cholestane-3β,4β,6α,8,15β,16β,28-heptol (2) and (24 S)-28- O-[2,4-di- O-methyl-β-D-xylopyranosyl-(1→2)-α-L-arabinofuranosyl]-24-methyl-5α-cholestane-3β,4β,6α,8,15β,16β,28-heptol 6- O-sulfate (3), respectively. Compound 2 is the first steroid glycoside containing an α-fucopyranose unit found from starfish. Compound 1 slightly inhibits cell proliferation of HCT-116, T-47D, and RPMI-7951 cancer cell lines, but has no effect on colony formation of these cells in a soft agar clonogenic assay.

A simple method of delayed processing of tumor tissue in a soft agar clonogenic assay

1988 ◽  
Vol 114 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Walter Krischke ◽  
Rolf Hartmann ◽  
Martin Schneider ◽  
Helmut Sch�nenberger
Keyword(s):  
Tumor Tissue ◽  
Clonogenic Assay ◽  
Soft Agar ◽  
Simple Method ◽  
Soft Agar Clonogenic Assay

scholarly journals Two New Asterosaponins from the Far Eastern Starfish Lethasterias fusca

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Natalia V. Ivanchina ◽  
Anatoly I. Kalinovsky ◽  
Alla A. Kicha ◽  
Timofey V. Malyarenko ◽  
Pavel S. Dmitrenok ◽  
...  
Keyword(s):  
Cell Lines ◽  
Clonogenic Assay ◽  
Ethanolic Extract ◽  
Soft Agar ◽  
Chemical Transformations ◽  
Hct 116 ◽  
New Compounds ◽  
Far Eastern ◽  
Soft Agar Clonogenic Assay ◽  
Cell Colony

Two new asterosaponins, lethasteriosides A (1) and B (2), were isolated along with previously known thornasteroside A (3), anasteroside A (4), and luidiaquinoside (5) from the ethanolic extract of the Far Eastern starfish Lethasterias fusca. The structures of the new compounds were elucidated by extensive NMR and ESIMS techniques, and chemical transformations. Compounds 1 and 3–5 did not show any apparent cytotoxicity against cancer cell lines T-47D, RPMI-7951, and HCT-116, but glycoside 1, at concentration of 20 μM, demonstrated considerable inhibition of the T-47D (97%), RPMI-7951 (90%) and HCT-116 (90%) cell colony formations in a soft agar clonogenic assay.

scholarly journals Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA.

1988 ◽  
Vol 8 (2) ◽  
pp. 588-594 ◽  
Author(s):  
R Sasada ◽  
T Kurokawa ◽  
M Iwane ◽  
K Igarashi
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Cellular Transformation ◽  
Soft Agar ◽  
Transformed Cells ◽  
Fibroblast Growth ◽  
Morphological Transformation ◽  
Phenotypic Alteration ◽  
Autocrine Stimulation

The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules.

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