Results of studying green crops of the Apiaceae family as genetic resources for vertical farming using natural immunomodulators

Relevance. New technologies Plenty-type in greenhouse vegetable production is wide spread in some countries of Europe, America, South-East Asia and support with main investors of these countries. Project “Vertical farming” was started in FSBSI “Federal Scientific Vegetable Center” at 2010. The goal of our study is the results of the testing plants Apiaceae family at multy circle hydroponic installation using the natural regulators from glycosides class.Materials and methods. Objects of study: plants Apiaceae family – Coriandrum sativum L. (variety Jubilar), Anethum graveolens L. (variety Rusich), Apium graveolens L. (variety Aeliksir); flavonoid glycoside linarozid from plants Linaria vulgaris Mill. L., steroid glycoside moldstim from seeds Capsicum annuum L.. Methods of study: seed treatment with 0,001% water solutions of glycosides, cultivation of plants Apiaceae family at the multi circle hydroponic construction.Results. First experiment by cultivation of plants Apiaceae family at multi circle hydroponic construction was successful and show, that plants Apiaceae family can cultivate at vertical installations Plenty-type. Reaction on the seed treatment with water solutions of glycosides is depended on the species of plants. Seed treatment with glycosides increased height of plants and weight of leaves of Coriandrum sativum L. Anethum graveolens L. increased germination of seeds after seed treatment with water solution of linarozide. Apium graveolens L. increased germination of seeds, height of plants and weight of leaves after seed treatment with water solutions of glycosides. But water solution of moldstim was more effective in 2020, and water solution of linarozide was more effective in 2021.

Thành phần hoá học và hoạt tính kháng vi khuẩn Vibrio parahaemolyticus của các cao chiết cây sổ trai (Dillenia ovata)

Nghiên cứu được thực hiện để đánh giá thành phần hoá học và hoạt tính kháng khuẩn của các cao chiết Sổ Trai. Thành phần hóa học của các cao chiết cây Sổ Trai được tìm thấy có chứa alkaloid, flavonoid, steroid, glycoside, saponin, tannin và sesquiterpene lactones. Cao chiết ethyl acetate của lá Sổ Trai và ethyl acatete của gỗ Sổ Trai có hàm lượng flavonoid và polyphenol cao, lần lượt là 309,97±1,47 mg QE/g cao chiết và 44,28±0,15 mg GAE/g cao chiết. Phương pháp khuếch tán trên giếng thạch được sử dụng để đánh giá hoạt tính kháng vi khuẩn Vibrio parahaemolyticus. Nồng độ ức chế tối thiểu (MIC) và nồng độ diệt khuẩn tối thiểu (MBC) được xác định thông qua sự thay đổi màu của resazurin trên đĩa 96 giếng. Kết quả cho thấy cao chiết ethyl acetate của lá Sổ Trai thể hiện hoạt tính kháng khuẩn Vibrio parahaemolyticus mạnh nhất, với nồng độ ức chế tối thiểu và nồng độ diệt khuẩn tối thiểu lần lượt là 320<MIC≤640 µg/mL, và 2560<MBC≤5120 µg/mL. Nghiên cứu này cho thấy tiềm năng của cây Sổ Trai trong phòng và điều trị bệnh do Vibrio parahaemolyticus gây ra.

Successful Treatment of Severe Digitoxin Intoxication with CytoSorb® Hemoadsorption

While several intoxications can be successfully treated with specific antidotes, intoxications with the steroid glycoside digitoxin still represent a major challenge. Besides conventional approaches, CytoSorb® hemoadsorption might be another treatment option. We report on an 81-year-old female patient treated in our intensive care unit (ICU) with severe digitoxin intoxication, acute renal failure, and urinary tract infection (UTI). As physiological digitoxin elimination kinetics are known to appear slow, and also in regard to the renal failure, the decision was made to initiate continuous renal replacement therapy combined with CytoSorb hemoadsorption. The patient was hemodynamically stabilized within the first 4 h of treatment and initially required catecholamines to be stopped within 24 h of treatment. Pre- and post-adsorber drug level measurements showed a rapid elimination of digitoxin. Antibiotic treatment with piperacillin/tazobactam was initiated, and despite CytoSorb hemoadsorption therapy and its known potential to reduce plasma concentrations of several drugs, the UTI was successfully treated. After 3 days of CytoSorb treatment, digitoxin plasma levels were stable and almost normalized, and no clinical signs of intoxication were present. Five days after presentation, the patient was transferred from the ICU in a stable condition. CytoSorb hemoadsorption may be an easily available, efficient, and less cost-intensive therapy option than treatment with the Fab fragment, which is the currently recommended therapy for digitalis intoxications. Therefore, the use of CytoSorb might represent an alternative treatment for life-threatening complications of digitoxin intoxications.

Polymorphism of morphometric parameters of seeds common anise and cumin

Relevance. The degree of variability of the linear parameters of the morphological elements of the seeds of the anise (variety Vityaz) and cumin (variety Peresvet).Materials and methods. 1) Plants: Monarda fistulosa L. (the breeding sample №5 U.P.), Monarda citriodora Cerv. ex Lag. (Simka variety), Melissa officinalis L. (Zhemchuzhina variety). 2) secondary metabolites: flavonoid glycoside linarozid and steroid glycoside moldstim.Methods. The studies were carried out in VNIIO – a branch of the FSBSI Federal Scientific Vegetable Center. The measurement of the length of the seed and endosperm was carried out using a caliper (GOST 166-89). The embryo length was determined using a Levenhuk 670T microscope and a DCM 300 MD video eyepiece at Ч 40 magnification using Scope Photo (Image Software V. 3.1.386). The lengths of each seed, endosperm (longitudinal section) and embryo (after isolation) were sequentially analyzed. The indices IE/C, IZ/E, IZ/C were calculated as the corresponding ratios of the length of the seed, endosperm and embryo.Results. The maximum length of the seed (4.76±0.10 mm) and of the endosperm (4.08 ± 0.08 mm) and the length of the embryo (1.40±0.02 mm) were noted in cumin seeds. The average values of the coefficient of variation (V), on the contrary, were minimal for cumin seeds, varying from 7.8% for the length of the embryo to 11.5% for the length of the seed. The average values of the coefficient of variation (V) for anise varied from 9.3% for the length of the embryo to 14.7% for the length of the seed. The maximum level of variability in both cultures was noted for seed size. Correlation analysis showed that a close relationship was observed in anise (r=0.912) and cumin seeds (r=0.876) only between the length of the seed and the length of the endosperm. The value of the correlation coefficient (r) between the linear sizes of the seed and the embryo was 0.195 for anise and 0.229 for cumin seeds, and between the length of the endosperm and the length of the embryo, 0.237 and 0.214, respectively. The correlation coefficient (r) between the index IZ/E and the linear sizes of the seed, endosperm and embryo had low negative values from -0.221 to - 0.345.

UJI EFEKTIVITAS SALEP EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L.) TERHADAP PENYEMBUHAN LUKA SAYAT PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

ABSTRACTJatropha leaves (Jatropha curcas L.) contains alkaloid, saponin, tannin, terpenoid, steroid, glycoside, phenol compound and flavonoid through ethanol extract. Until today the community still used the plant as a wound healing medicine. The aim of this study is to examine the effectiveness of jatropha curcas L. leaf extract ointment for healing in male white rats wistar strain. Jatropha leaves extract ointment was tested on 5 which consists of 3 rats in each treatment groups, that is base ointment (negative control), ointment betadine (positive control), ointment extract of Jatropha leaves 10%, 20% and 40%. Observations has made until the 14th day. And data were tested using ANOVA (Analysis of Variants) and continued with LSD (Least Significant Different) test. Statistical test results showed a difference in the duration of wound healing in male white rats wistar strain between the ointment extract of jatropha leaf with ointment betadine and vaseline ointment. Keywords: Ointment, Jatropha extract (Jatropha curcas L.), Galur wistar, Wound.ABSTRAK Daun jarak pagar (Jatropha curcas L.) mengandung zat-zat alkaloid, saponin, tannin, terpenoid, steroid, glikosida, senyawa fenol dan flavonoid melalui ekstrak etanol. Hingga saat ini masyarakat masih menggunakan tanaman tersebut sebagai obat penyembuh luka. Penelitian ini bertujuan untuk menguji efektivitas salep ekstrak etanol daun jarak pagar terhadap penyembuhan luka sayat pada tikus putih jantan galur wistar. Salep ekstrak daun jarak pagar diujikan pada hewan uji tikus sebanyak 5 yang terdiri dari 3 ekor tikus disetiap kelompok perlakuan, yaitu basis salep (kontrol negatif), salep betadine (kontrol positif), salep ekstrak etanol daun jarak pagar 10%, 20% dan 40%. Pengamatan dilakukan sampai hari ke 14. Data diuji dengan menggunakan ANOVA (Analisis Of Variant) dan dilanjutkan dengan uji LSD (Least Significant Different). Hasil uji statistik menunjukan adanya perbedaan terhadap lamanya penyembuhan luka pada tikus putih jantan galur wistar antara sediaan salep ekstrak daun jarak pagar dengan salep salep betadine dan basis salep. Kata Kunci: Salep, Daun jarak pagar (Jatropha curcas L.), Galur wistar, Luka sayat.

Bioactive Compounds Isolated from Indonesian Epiphytic Plant of Sarang Semut and Their Antibacterial Activity against Pathogenic Oral Bacteria

A new phenolic compound (1), a steroid (2), a new steroid glycoside (4), two triterpenoids (3 & 6) and a new phloroglucinol-sesquiterpene (5) have been isolated from the ethyl acetate extract of Sarang Semut ( Myrmecodia pendans) and their structures were determined on the basis of the spectral data. The bioactivity evaluation was conducted with the inhibition zone of compounds (mm) using Kirby-Bauer method at concentrations of 1000 and 5000 ppm for compound 1 against pathogenic oral bacteria Enterococcus faecalis, was 8.55 and 8.05 mm, respectively. Compounds 2–3 against Streptococcus mutans were 9.00 and 8.45 mm (2) and 10.24 and 9.35 mm (3), respectively. Compound 5 against Porphyromonas gingivalis was 11.5 and 10.8 mm, respectively.

Evaluation of cytotoxic, analgesic, antidiarrheal and phytochemical properties of Hygrophila spinosa (T. Anders) whole plant

Background:Synthetic drugs are going to be replaced by plant-derived traditional drugs due to their cost effectiveness, relatively less harmfulness, and efficacy against multidrug resistance organisms.Methods:Preliminary phytochemical screening was accomplished by established methods modified in experimental protocol. EExHs was undertaken for cytotoxic assay by Brine shrimp lethality bioassay, antinociceptive action by acetic acid induced writhing test, and antidiarrheal activity by castor oil induced antidiarrheal test. Data were analyzed by GraphPad Prism 6.0 software using Dunnett’s test for multiple comparisons.Results:Reducing sugar, steroid, glycoside, tannin, alkaloid, saponins, and flavonoids were found to be present in EExHs. Lethal concentration (LCConclusions:This research demonstrates that the secondary metabolites guided cytotoxic and analgesic effects could be extensively studied in multiple models to confirm the effects.

IDENTIFIKASI SENYAWA KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK Piper Sp. ASAL PAPUA

The aim of this study was to identity the chemical compounds of the piper sp.Fruit originated from Papua, that has been used as “sirih pinang”(chew betle)and to determine the antibacterial activity of hexane and chloroform extraxt ofpiper sp. to Gram + bacteria isolates(Bacillus subtilis, Listeria monocytogenesdan Staphylococcus aureus) and Gram – bacteria isolates(Salmonellatyphimurium, Eschericia coli and Pseudomonas pseudomallei). The extractconcentration were 50 % and 25 %, and every treatment consisted of 3replications. The bacteriae growth media was Mueller Hinton Agar(MHA). Theresult of phytochemical screening of Piper sp.fruit(ether, alcohol,and waterextracts)showed that it contained esential oil, lipid,emodol,tannin,reducingsugar, anthrasenoid,polyuronide,glucocide,steroid glycoside.