scholarly journals Targeting DXP synthase in human pathogens: enzyme inhibition and antimicrobial activity of butylacetylphosphonate

2013 ◽  
Vol 67 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Jessica M Smith ◽  
Nicole V Warrington ◽  
Ryan J Vierling ◽  
Misty L Kuhn ◽  
Wayne F Anderson ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enzyme Inhibition ◽  
Human Pathogens ◽  
Dxp Synthase

scholarly journals Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 411
Author(s):  
Maxence Quemener ◽  
Marie Dayras ◽  
Nicolas Frotté ◽  
Stella Debaets ◽  
Christophe Le Meur ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Oceanic Crust ◽  
Gene Clusters ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Human Pathogens ◽  
Biotechnological Potential ◽  
Fungal Isolates

Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their ability to express specific biosynthetic gene clusters (BGCs) involved in the synthesis of antimicrobial compounds. Here, a collection of unique 75 deep oceanic crust fungi was screened to evaluate their biotechnological potential through the prism of their antimicrobial activity using a polyphasic approach. After a first genetic screening to detect specific BGCs, a second step consisted of an antimicrobial screening that tested the most promising isolates against 11 microbial targets. Here, 12 fungal isolates showed at least one antibacterial and/or antifungal activity (static or lytic) against human pathogens. This analysis also revealed that Staphylococcus aureus ATCC 25923 and Enterococcus faecalis CIP A 186 were the most impacted, followed by Pseudomonas aeruginosa ATCC 27853. A specific focus on three fungal isolates allowed us to detect interesting activity of crude extracts against multidrug-resistant Staphylococcus aureus. Finally, complementary mass spectrometry (MS)-based molecular networking analyses were performed to putatively assign the fungal metabolites and raise hypotheses to link them to the observed antimicrobial activities.

scholarly journals Comparison of Methods of Extraction and Antimicrobial Activity of Six Ocimum Species against Human Pathogens

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Manjula Bomma ◽  
Florence Okafor ◽  
S.R. Mentreddy ◽  
Leopold Nyochembeng ◽  
William Setzer ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Comparison Of Methods ◽  
Human Pathogens ◽  
Ocimum Species

The structure of urease inactivated by Ag(i): a new paradigm for enzyme inhibition by heavy metals

2018 ◽  
Vol 47 (25) ◽  
pp. 8240-8247 ◽  
Author(s):  
Luca Mazzei ◽  
Michele Cianci ◽  
Antonio Gonzalez Vara ◽  
Stefano Ciurli
Keyword(s):  
Crystal Structure ◽  
Heavy Metals ◽  
Enzyme Inhibition ◽  
Virulence Factor ◽  
Soil Nitrogen ◽  
Nitrogen Fertilization ◽  
Enzyme Complex ◽  
Human Pathogens ◽  
New Paradigm

The molecular details of the inactivation of urease, a nickel-dependent virulence factor for human pathogens and negatively affecting the efficiency of soil nitrogen fertilization, are elucidated through the crystal structure of the enzyme complex with Ag(i).

scholarly journals Production of an Engineered Killer Peptide in Nicotiana benthamiana by Using a Potato virus X Expression System

2005 ◽  
Vol 71 (10) ◽  
pp. 6360-6367 ◽  
Author(s):  
Marcello Donini ◽  
Chiara Lico ◽  
Selene Baschieri ◽  
Stefania Conti ◽  
Walter Magliani ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pseudomonas Syringae ◽  
Potato Virus ◽  
Broad Spectrum ◽  
Plant Pathogens ◽  
Expression System ◽  
Potato Virus X ◽  
Scale Production ◽  
Human Pathogens ◽  
Single Chain

ABSTRACT The decapeptide killer peptide (KP) derived from the sequence of a single-chain, anti-idiotypic antibody acting as a functional internal image of a microbicidal, broad-spectrum yeast killer toxin (KT) was shown to exert a strong microbicidal activity against human pathogens. With the aim to exploit this peptide to confer resistance to plant pathogens, we assayed its antimicrobial activity against a broad spectrum of phytopathogenic bacteria and fungi. Synthetic KP exhibited antimicrobial activity in vitro towards Pseudomonas syringae, Erwinia carotovora, Botrytis cinerea, and Fusarium oxysporum. KP was also expressed in plants by using a Potato virus X (PVX)-derived vector as a fusion to the viral coat protein, yielding chimeric virus particles (CVPs) displaying the heterologous peptide. Purified CVPs showed enhanced antimicrobial activity against the above-mentioned plant pathogens and human pathogens such as Staphylococcus aureus and Candida albicans. Moreover, in vivo assays designed to challenge KP-expressing plants (as CVPs) with Pseudomonas syringae pv. tabaci showed enhanced resistance to bacterial attack. The results indicate that the PVX-based display system is a high-yield, rapid, and efficient method to produce and evaluate antimicrobial peptides in plants, representing a milestone for the large-scale production of high-added-value peptides through molecular farming. Moreover, KP is a promising molecule to be stably engineered in plants to confer broad-spectrum resistance to phytopathogens.

Thermo-chemical induced production of silver nanoparticles (Ag-NPs) and their antimicrobial activity towards human pathogens

2017 ◽  
Vol 10 (5) ◽  
pp. 1322
Author(s):  
S. Krishnakumar ◽  
R. Divya ◽  
N.R. Kanchana Devi ◽  
G. Keerthana ◽  
A. Ancy Judi
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Human Pathogens ◽  
Ag Nps

Synthesis, Antimicrobial and β-Lactamase Enzyme Inhibition Activity of Some New Tetrazole Containing Maleamic and Phthaleamic Acid Derivatives

2019 ◽  
Vol 4 (3) ◽  
pp. 166-173
Author(s):  
Bhaurao P. Sathe ◽  
Harshal S. Oman ◽  
Naziya N.M.A. Rehman ◽  
Prashant P. Dixit ◽  
Arun S. Kharat ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enzyme Inhibition ◽  
1H Nmr ◽  
13C Nmr ◽  
Enzyme Inhibitors ◽  
Inhibition Activity ◽  
Spectral Techniques

In the present study, two series of tetrazole containing maleamic (5a-h) and phthaleamic acid (5i-l) derivatives were synthesized and evaluated for their antimicrobial and β-lactamase enzyme inhibition activities. The synthesized compounds were characterized by IR, 1H NMR and 13C NMR spectral techniques. Among the screened compounds, the compound 5c, 5d, 5e, 5f, 5g and 5h have shown good antimicrobial activity. We further performed exploratory β-lactamase enzyme inhibitors studies on β-lactamase.

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