scholarly journals How Does a Skin Fibroblast Make Type I Collagen Fibers?

1982 ◽  
Vol 79 (1) ◽  
pp. 3-6 ◽  
Author(s):  
Darwin J. Prockop
Keyword(s):  
Skin Fibroblast ◽  
Type I Collagen ◽  
Collagen Fibers ◽  
Type I

Assembly of Type I Collagen on PVA Film Induced by Glutaraldehyde Vapor

2011 ◽  
Vol 284-286 ◽  
pp. 1794-1799 ◽  
Author(s):  
Yu Lu Wang ◽  
Xue Pin Liao ◽  
Bi Shi
Keyword(s):  
Network Structure ◽  
Type I Collagen ◽  
Collagen Fibers ◽  
Type I ◽  
Collagen Concentration ◽  
Collagen Molecules ◽  
Induced Reaction ◽  
Calf Skin

Type I collagen was isolated from calf skin and its assembly on PVA film induced by glutaraldehyde vapor was investigated. It was found that the collagen molecules were firstly orientationally assembled into collagen fibers under the inducement of glutaraldehyde vapor. Then the collagen fibers could be further aggregated into novel network structure in proper conditions of the induced reaction. The morphology of the assembled collagen fibers was depended on induced time and concentration of collagen. The network arrangement could be obtained after being induced for 72h when collagen concentration was 2.5mg/ml. At higher concentration of collagen (5 mg/ml), the collagen fibers with larger dimension were obtained, but the growth of fibers was almost in one direction.

scholarly journals Inhibition of Collagen-Induced Platelet Aggregation by Antibodies to Distinct Types of Collagen

1977 ◽  
Author(s):  
L. Balleisen ◽  
R. Timpl ◽  
S. Gay
Keyword(s):  
Platelet Aggregation ◽  
Serum Albumin ◽  
Type I Collagen ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Fibrillar Collagen ◽  
Type Ii ◽  
Molecular Parameters ◽  
Inhibition Reaction

The reaction of platelets with fibrillar collagen was measured by recording aggregation according to Borns method and by retraction of Ancrod-fibrin clots. These reactions could be completely inhibited by coating the fibrils with stoichiometric amounts of purified antibodies to type I, II or III collagens. The inhibition was specific, i. e. antibodies to type I collagen prevented aggregation by type I collagen but not by type II or III collagen. Comparable amounts ofantibodies to fibrinogen or to serum albumin had no effect on the reaction. The data indicate that platelet aggregation by type I or II collagen fibrils is not due to contamination with type III collagen. The inhibition reaction may be useful for further studies on molecular parameters of the interaction between platelets and collagen fibers.

Hydrogen peroxide-induced degradation of type I collagen fibers of tilapia skin

2014 ◽  
Vol 2 (1-2) ◽  
pp. 41-48 ◽  
Author(s):  
Xiaoling Liu ◽  
Yuanxin Jiang ◽  
Hong He ◽  
Wei Ping
Keyword(s):  
Hydrogen Peroxide ◽  
Type I Collagen ◽  
Collagen Fibers ◽  
Type I

scholarly journals Incorporation of Superparamagnetic Iron Oxide Nanoparticles into Collagen Formulation for 3D Electrospun Scaffolds

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 181
Author(s):  
Manuel Estévez ◽  
Giorgia Montalbano ◽  
Alvaro Gallo-Cordova ◽  
Jesús G. Ovejero ◽  
Isabel Izquierdo-Barba ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Type I Collagen ◽  
Superparamagnetic Iron Oxide ◽  
Collagen Fibers ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Biological Assessment ◽  
Oxide Nanoparticles ◽  
Type I ◽  
Electrospun Scaffolds

Nowadays, there is an ever-increasing interest in the development of systems able to guide and influence cell activities for bone regeneration. In this context, we have explored for the first time the combination of type-I collagen and superparamagnetic iron oxide nanoparticles (SPIONs) to design magnetic and biocompatible electrospun scaffolds. For this purpose, SPIONs with a size of 12 nm were obtained by thermal decomposition and transferred to an aqueous medium via ligand exchange with dimercaptosuccinic acid (DMSA). The SPIONs were subsequently incorporated into type-I collagen solutions to prove the processability of the resulting hybrid formulation by means of electrospinning. The optimized method led to the fabrication of nanostructured scaffolds composed of randomly oriented collagen fibers ranging between 100 and 200 nm, where SPIONs resulted distributed and embedded into the collagen fibers. The SPIONs-containing electrospun structures proved to preserve the magnetic properties of the nanoparticles alone, making these matrices excellent candidates to explore the magnetic stimuli for biomedical applications. Furthermore, the biological assessment of these collagen scaffolds confirmed high viability, adhesion, and proliferation of both pre-osteoblastic MC3T3-E1 cells and human bone marrow-derived mesenchymal stem cells (hBM-MSCs).

Platelet Uptake On Bioprosthetic Heart Valves

1981 ◽  
Author(s):  
C Oyama ◽  
S Klein ◽  
D J Magilligan
Keyword(s):  
Heart Valves ◽  
Type I Collagen ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Primary Role ◽  
Standard Size ◽  
Consistent Finding ◽  
Bioprosthetic Heart Valves ◽  
Platelet Adherence

Single and aggregated platelets have been a consistent finding on the surface of 28 degenerated porcine bioprosthetic heart valves. Both the degenerated valves as well as unimplanted commercially available valves exhibit loss of endocardium with bare collagen fibers on scanning electron microscopy. The role of platelet adherence to the collagen fibers of these glutaraldehyde treated bioprostheses in causing degeneration is an unanswered question. Platelet adherence to porcine (P) valve (Type III collagen) and bovine (B) pericardium (Type I collagen) was measured in the glutaraldehyde treated bioprosthesis and in the fresh state with saline (F, S) and with plasma (F, P). Single cusps or similar samples of pericardium were rotated at 200 RPM for 10 min in a concentration of human platelets tagged with 51Cr. The tissue was trimmed to standard size, counted in a gamma counter and adherence calculated as platelets (pits)/mm2.Our experimental observations did not show a difference in platelet adherence between porcine or bovine tissue either G-treated or F. The G-treated valves showed significantly less (p=<.01) adherence than the F tissue. Although G- treated bioprostheses have collagen fibers exposed to circulating blood, platelet collagen reaction probably does not play a primary role in degeneration of the bioprosthetic heart valve.

scholarly journals Microextrusion printing cell-laden networks of type I collagen with patterned fiber alignment and geometry

Soft Matter ◽  
2019 ◽  
Vol 15 (28) ◽  
pp. 5728-5738 ◽  
Author(s):  
Bryan A. Nerger ◽  
P.-T. Brun ◽  
Celeste M. Nelson
Keyword(s):  
Type I Collagen ◽  
Collagen Fibers ◽  
Type I ◽  
Fiber Alignment

Cell-laden networks of aligned type I collagen fibers are fabricated using 3D microextrusion printing of collagen-Matrigel inks.

Collagen-based biocomposites inspired by bone hierarchical structures advanced bone regeneration: Ongoing research and perspectives

2021 ◽  
Author(s):  
Di Qin ◽  
Na Wang ◽  
Xin-Guo You ◽  
Andi Zhang ◽  
Xiguang Chen ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Regeneration ◽  
Hierarchical Structure ◽  
Type I Collagen ◽  
Hierarchical Structures ◽  
Collagen Fibers ◽  
Type I ◽  
Ongoing Research ◽  
The Matrix

Bone is a hard-connective tissue composed of matrix, cells and bioactive factors with hierarchical structure, where the matrix is mainly composed of type I collagen and hydroxyapatite. Collagen fibers assembled...

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