scholarly journals EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Ningning Niu ◽  
Wanqi Liang ◽  
Xijia Yang ◽  
Weilin Jin ◽  
Zoe A. Wilson ◽  
...  
Keyword(s):  
Cell Death ◽  
Tapetal Cell ◽  
Reproductive Development ◽  
Aspartic Proteases

scholarly journals DCET1 Controls Male Sterility Through Callose Regulation, Exine Formation, and Tapetal Programmed Cell Death in Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Riaz Muhammad Khan ◽  
Ping Yu ◽  
Lianping Sun ◽  
Adil Abbas ◽  
Liaqat Shah ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Male Sterility ◽  
Tapetal Cell ◽  
Pollen Wall ◽  
Rna Recognition Motif ◽  
Male Sterile ◽  
Cell Degeneration ◽  
Regulatory Pathways ◽  
Callose Wall

In angiosperms, anther development comprises of various complex and interrelated biological processes, critically needed for pollen viability. The transitory callose layer serves to separate the meiocytes. It helps in primexine formation, while the timely degradation of tapetal cells is essential for the timely callose wall dissolution and pollen wall formation by providing nutrients for pollen growth. In rice, many genes have been reported and functionally characterized that are involved in callose regulation and pollen wall patterning, including timely programmed cell death (PCD) of the tapetum, but the mechanism of pollen development largely remains ambiguous. We identified and functionally characterized a rice mutant dcet1, having a complete male-sterile phenotype caused by defects in anther callose wall, exine patterning, and tapetal PCD. DCET1 belongs to the RNA recognition motif (RRM)-containing family also called as the ribonucleoprotein (RNP) domain or RNA-binding domain (RBD) protein, having single-nucleotide polymorphism (SNP) substitution from G (threonine-192) to A (isoleucine-192) located at the fifth exon of LOC_Os08g02330, was responsible for the male sterile phenotype in mutant dcet1. Our cytological analysis suggested that DCET1 regulates callose biosynthesis and degradation, pollen exine formation by affecting exine wall patterning, including abnormal nexine, collapsed bacula, and irregular tectum, and timely PCD by delaying the tapetal cell degeneration. As a result, the microspore of dcet1 was swollen and abnormally bursted and even collapsed within the anther locule characterizing complete male sterility. GUS and qRT-PCR analysis indicated that DCET1 is specifically expressed in the anther till the developmental stage 9, consistent with the observed phenotype. The characterization of DCET1 in callose regulation, pollen wall patterning, and tapetal cell PCD strengthens our knowledge for knowing the regulatory pathways involved in rice male reproductive development and has future prospects in hybrid rice breeding.

scholarly journals Transcriptional Regulation and Signaling of Developmental Programmed Cell Death in Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Cheng Jiang ◽  
Jiawei Wang ◽  
Hua-Ni Leng ◽  
Xiaqin Wang ◽  
Yijing Liu ◽  
...  
Keyword(s):  
Cell Death ◽  
Transcriptional Regulation ◽  
Programmed Cell Death ◽  
Industrial Production ◽  
Recent Progress ◽  
Reproductive Development ◽  
Genetic Networks ◽  
Molecular Regulation ◽  
Molecular Networks ◽  
Plant Growth And Development

Developmental programmed cell death (dPCD) has multiple functions in plant growth and development, and is of great value for industrial production. Among them, wood formed by xylem dPCD is one of the most widely used natural materials. Therefore, it is crucial to explore the molecular mechanism of plant dPCD. The dPCD process is tightly regulated by genetic networks and is involved in the transduction of signaling molecules. Several key regulators have been identified in diverse organisms and individual PCD events. However, complex molecular networks controlling plant dPCD remain highly elusive, and the original triggers of this process are still unknown. This review summarizes the recent progress on the transcriptional regulation and signaling of dPCD during vegetative and reproductive development. It is hoped that this review will provide an overall view of the molecular regulation of dPCD in different developmental processes in plants and identify specific mechanisms for regulating these dPCD events. In addition, the application of plants in industrial production can be improved by manipulating dPCD in specific processes, such as xylogenesis.

scholarly journals OsGPAT3 Plays a Critical Role in Anther Wall Programmed Cell Death and Pollen Development in Rice

2018 ◽  
Vol 19 (12) ◽  
pp. 4017 ◽  
Author(s):  
Lianping Sun ◽  
Xiaojiao Xiang ◽  
Zhengfu Yang ◽  
Ping Yu ◽  
Xiaoxia Wen ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Male Sterility ◽  
Critical Role ◽  
Pollen Grains ◽  
Reproductive Development ◽  
Anther Wall ◽  
Nutrient Metabolism ◽  
Pollen Maturation ◽  
Pollen Formation

In flowering plants, ideal male reproductive development requires the systematic coordination of various processes, in which timely differentiation and degradation of the anther wall, especially the tapetum, is essential for both pollen formation and anther dehiscence. Here, we show that OsGPAT3, a conserved glycerol-3-phosphate acyltransferase gene, plays a critical role in regulating anther wall degradation and pollen exine formation. The gpat3-2 mutant had defective synthesis of Ubisch bodies, delayed programmed cell death (PCD) of the inner three anther layers, and abnormal degradation of micropores/pollen grains, resulting in failure of pollen maturation and complete male sterility. Complementation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) experiments demonstrated that OsGPAT3 is responsible for the male sterility phenotype. Furthermore, the expression level of tapetal PCD-related and nutrient metabolism-related genes changed significantly in the gpat3-2 anthers. Based on these genetic and cytological analyses, OsGPAT3 is proposed to coordinate the differentiation and degradation of the anther wall and pollen grains in addition to regulating lipid biosynthesis. This study provides insights for understanding the function of GPATs in regulating rice male reproductive development, and also lays a theoretical basis for hybrid rice breeding.

scholarly journals PERSISTENT TAPETAL CELL1 Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

2011 ◽  
Vol 156 (2) ◽  
pp. 615-630 ◽  
Author(s):  
Hui Li ◽  
Zheng Yuan ◽  
Gema Vizcay-Barrena ◽  
Caiyun Yang ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Cell Death ◽  
Pollen Development ◽  
Tapetal Cell ◽  
Phd Finger ◽  
Finger Protein

scholarly journals Regulation of Vacuole-Mediated Programmed Cell Death During Innate Immunity and Reproductive Development in Plants

2014 ◽  
pp. 431-440 ◽  
Author(s):  
Tomoko Koyano ◽  
Takamitsu Kurusu ◽  
Shigeru Hanamata ◽  
Kazuyuki Kuchitsu
Keyword(s):  
Innate Immunity ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Reproductive Development

scholarly journals The mitochondrial aldehyde dehydrogenase OsALDH2b negatively regulates tapetum degeneration in rice

2020 ◽  
Vol 71 (9) ◽  
pp. 2551-2560 ◽  
Author(s):  
Xianrong Xie ◽  
Zixu Zhang ◽  
Zhe Zhao ◽  
Yongyao Xie ◽  
Heying Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Oryza Sativa ◽  
Programmed Cell Death ◽  
Aldehyde Dehydrogenase ◽  
Molecular Basis ◽  
Reproductive Development ◽  
Flowering Plants ◽  
Microspore Development ◽  
Tapetal Cells ◽  
Microspore Stage

Abstract Timely degradation of anther tapetal cells is a prerequisite for normal pollen development in flowering plants. Although several genes involved in tapetum development have been identified, the molecular basis of tapetum degeneration regulation remains poorly understood. In this study, we identified and characterized the nucleus-encoded, conserved mitochondrial aldehyde dehydrogenase OsALDH2b as a key regulator of tapetum degeneration in rice (Oryza sativa). OsALDH2b was highly expressed in anthers from meiosis to the early microspore stage. Mutation of OsALDH2b resulted in excess malonaldehyde accumulation and earlier programmed cell death in the tapetum, leading to premature tapetum degeneration and abnormal microspore development. These results demonstrate that OsALDH2b negatively regulates tapetal programmed cell death and is required for male reproductive development, providing insights into the regulation of tapetum development in plants.

scholarly journals The Rice Receptor-Like Kinases DWARF AND RUNTISH SPIKELET1 and 2 Repress Cell Death and Affect Sugar Utilization during Reproductive Development

2017 ◽  
Vol 29 (1) ◽  
pp. 70-89 ◽  
Author(s):  
Cui-Xia Pu ◽  
Yong-Feng Han ◽  
Shu Zhu ◽  
Feng-Yan Song ◽  
Ying Zhao ◽  
...  
Keyword(s):  
Cell Death ◽  
Reproductive Development ◽  
Sugar Utilization

Control of Programmed Cell Death During Plant Reproductive Development

2011 ◽  
pp. 171-196 ◽  
Author(s):  
Yadira Olvera-Carrillo ◽  
Yuliya Salanenka ◽  
Moritz K. Nowack
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Reproductive Development ◽  
Plant Reproductive Development
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