Enrichment of extracellular vesicles using Millipore membrane filters followed by isolation of exosomal RNA from serum or plasma using the Qiagen miRNeasy Micro kit

2017 ◽  
Author(s):  
Roger P. Alexander ◽  
Leonora Balaj ◽  
Justyna Filant ◽  
Parham Nejad ◽  
Anu Paul ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Membrane Filters ◽  
Millipore Membrane ◽  
Qiagen Mirneasy

scholarly journals Enrichment of extracellular vesicles using Millipore membrane filters

2015 ◽  
Author(s):  
Roger P. Alexander ◽  
Louise C. Laurent ◽  
Roger P. Alexander
Keyword(s):  
Extracellular Vesicles ◽  
Membrane Filters ◽  
Millipore Membrane

ELECTRON MICROSCOPIC STUDY OF POPULATIONS OF YEAST-LIKE FUNGI CANDIDA ALBICANS

2021 ◽  
Vol 1 (4) ◽  
pp. 459-467
Author(s):  
Inna B. Pavlova ◽  
◽  
Anna Yu. Arsenyuk ◽  
Daria A. Bannikova ◽  
Dmitry V. Gruznov ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Electron Microscope ◽  
Museum Collection ◽  
Electron Microscopic ◽  
Membrane Filters ◽  
Stage Of Development ◽  
Development Cycle ◽  
Phases Of Development ◽  
Bacteria And Fungi ◽  
Millipore Membrane

The article presents the results of electron microscopic investigations of populations of pathogenic Candida albicans № 138 from the museum collection. The morphology of the fungus, its ultrastructure and phases of development in the population were studied. The methods developed at VNIIVSGE for growing bacteria and fungi on the surfaces of Millipore membrane filters without disturbing the architectonics of populations were used in research. The preparations were examined with electron microscope Hitachi 800 (Japan) with two systems: transmission and scanning. At large magnifications, the features of the ultrastructure of Candida albicans cells were studied. It was found that in addition to budding, Candida albicans has a survival strategy in the form of the formation of small blastospore cells, the structure of which is visible only with the use of a scanning electron microscope. The forms, sizes and localization of blastospores with their own development cycle have been studied. The morphology of pseudogyphs, which play an important role in the existence and survival of fungi population, has been studied. The formation of biofilms at a certain stage of development of Candida albicans population was revealed.

Scanning electron microscopy of L-phase streptococci. II. Growth in broth and upon Millipore filters

1972 ◽  
Vol 18 (8) ◽  
pp. 1179-1184 ◽  
Author(s):  
David J. Bibel ◽  
John W. Lawson
Keyword(s):  
Electron Microscopy ◽  
Agar Medium ◽  
Lag Phase ◽  
Membrane Filters ◽  
Group A ◽  
Osmotic Lysis ◽  
Millipore Membrane ◽  
Group D ◽  
Increased Susceptibility ◽  
Millipore Filters

The growth of L-phase streptococci in broth and upon Millipore membrane filters was studied by light and scanning-beam electron microscopy. Osmotic sensitivity, shape, size, and surface features of L-phase bodies, ratios of large bodies to granules, and number of units in clusters varied with the phase of growth in broth. During the lag phase of growth the L-phase elements seemed to possess a weakened membrane as suggested by their increased susceptibility to osmotic lysis and their flattened shape. Irregular protuberances, which may be associated with formation of elementary corpuscles or degeneration of membrane, were found on large bodies in stationary phase. Replication in broth appeared to occur by single budding of large bodies or binary fission of granules measuring 0.7 μm or more in diameter. L-Phase colonies of group D streptococci, strains F24 and Z30, and group A streptococci, strains ADA, AED, and GL8, were differentiated by morphology when grown on Millipore membrane filters pressed upon uniform agar medium. Both group and strain variation were observed.

scholarly journals Statistical Analysis of the Recovery of Coliform Organisms on Gelman and Millipore Membrane Filters

1974 ◽  
Vol 28 (4) ◽  
pp. 605-607
Author(s):  
David J. Schaeffer ◽  
Maxine C. Long ◽  
Konanur G. Janardan
Keyword(s):  
Statistical Analysis ◽  
Membrane Filters ◽  
Millipore Membrane

An evaluation of three agents for eluting adsorbed enterovirus from millipore membrane filters

1971 ◽  
Vol 17 (11) ◽  
pp. 1351-1355 ◽  
Author(s):  
Jack Konowalchuk ◽  
Joan I. Speirs
Keyword(s):  
Yeast Extract ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Cellulose Nitrate ◽  
Membrane Filters ◽  
Beef Extract ◽  
Fetal Bovine ◽  
Millipore Membrane ◽  
High Degree

Several concentrations of yeast extract, beef extract, and fetal bovine serum were compared for ability to elute adsorbed coxsackievirus B5 and poliovirus 1 from HA cellulose nitrate Millipore membrane filters. Elution improved with increases in concentration of eluting agent and volume of eluent. The eluent of choice was undiluted fetal bovine serum because it was compatible with cells and showed a high degree of elution. A 5-ml serum eluate resulted in the recovery of 80–90% of the virus adsorbed by a filter from an infected buffered saline sample.

scholarly journals Statistical Analysis of the Recovery of Coliform Organisms on Gelman and Millipore Membrane Filters

1974 ◽  
Vol 28 (4) ◽  
pp. 605-607 ◽  
Author(s):  
David J. Schaeffer ◽  
Maxine C. Long ◽  
Konanur G. Janardan
Keyword(s):  
Statistical Analysis ◽  
Membrane Filters ◽  
Millipore Membrane

Transfer Technique for Electron Microscopy of Membrance Filter Samples

1974 ◽  
Vol 32 ◽  
pp. 554-555
Author(s):  
Lawrence W. Ortiz ◽  
Bonnie L. Isom
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Pore Size ◽  
Membrane Filters ◽  
Size Dependent

A procedure is described for the quantitative transfer of fibers and particulates collected on membrane filters to electron microscope (EM) grids. Various Millipore MF filters (Millipore AA, HA, GS, and VM; 0.8, 0.45, 0.22 and 0.05 μm mean pore size) have been used with success. Observed particle losses have not been size dependent and have not exceeded 10%. With fibers (glass or asbestos) as the collected media this observed loss is approximately 3%.

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