scholarly journals A Comparison of Seitz and Millipore Membrane Filters for the Enumeration of Fecal Coliforms

1976 ◽  
Vol 17 (1) ◽  
pp. 25-31
Author(s):  
Henry Søgaard
Keyword(s):  
Fecal Coliforms ◽  
Membrane Filters ◽  
Millipore Membrane

ELECTRON MICROSCOPIC STUDY OF POPULATIONS OF YEAST-LIKE FUNGI CANDIDA ALBICANS

2021 ◽  
Vol 1 (4) ◽  
pp. 459-467
Author(s):  
Inna B. Pavlova ◽  
◽  
Anna Yu. Arsenyuk ◽  
Daria A. Bannikova ◽  
Dmitry V. Gruznov ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Electron Microscope ◽  
Museum Collection ◽  
Electron Microscopic ◽  
Membrane Filters ◽  
Stage Of Development ◽  
Development Cycle ◽  
Phases Of Development ◽  
Bacteria And Fungi ◽  
Millipore Membrane

The article presents the results of electron microscopic investigations of populations of pathogenic Candida albicans № 138 from the museum collection. The morphology of the fungus, its ultrastructure and phases of development in the population were studied. The methods developed at VNIIVSGE for growing bacteria and fungi on the surfaces of Millipore membrane filters without disturbing the architectonics of populations were used in research. The preparations were examined with electron microscope Hitachi 800 (Japan) with two systems: transmission and scanning. At large magnifications, the features of the ultrastructure of Candida albicans cells were studied. It was found that in addition to budding, Candida albicans has a survival strategy in the form of the formation of small blastospore cells, the structure of which is visible only with the use of a scanning electron microscope. The forms, sizes and localization of blastospores with their own development cycle have been studied. The morphology of pseudogyphs, which play an important role in the existence and survival of fungi population, has been studied. The formation of biofilms at a certain stage of development of Candida albicans population was revealed.

Improved recovery of fecal coliforms from the Ottawa River by membrane filters in the presence of food debris

1985 ◽  
Vol 31 (1) ◽  
pp. 16-18 ◽  
Author(s):  
Jeffrey M. Farber ◽  
Anthony N. Sharpe ◽  
Miloslav Kalab
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
River Water ◽  
Water Samples ◽  
Fecal Coliform ◽  
Fecal Coliforms ◽  
Normal Stresses ◽  
Membrane Filters ◽  
Ottawa River ◽  
Scanning Electron

In the absence of food debris, Sartorius and Millipore HA filters recovered substantially fewer fecal coliforms from Ottawa River water than did Millipore HC. On addition of a small quantity of sterile blended carrot to water samples, recovery by the poorer filters equalled that on Millipore HC. Scanning electron microscopy revealed bacteria sheltered in crevices formed by carrot fibres and thus protected from the normal stresses of exposure. Addition of carrot debris (e.g., 0.03 g carrot to 100 mL of sample) thus provides a convenient and inexpensive means of reducing variations in fecal coliform recovery between brands of membrane filters.

Scanning electron microscopy of L-phase streptococci. II. Growth in broth and upon Millipore filters

1972 ◽  
Vol 18 (8) ◽  
pp. 1179-1184 ◽  
Author(s):  
David J. Bibel ◽  
John W. Lawson
Keyword(s):  
Electron Microscopy ◽  
Agar Medium ◽  
Lag Phase ◽  
Membrane Filters ◽  
Group A ◽  
Osmotic Lysis ◽  
Millipore Membrane ◽  
Group D ◽  
Increased Susceptibility ◽  
Millipore Filters

The growth of L-phase streptococci in broth and upon Millipore membrane filters was studied by light and scanning-beam electron microscopy. Osmotic sensitivity, shape, size, and surface features of L-phase bodies, ratios of large bodies to granules, and number of units in clusters varied with the phase of growth in broth. During the lag phase of growth the L-phase elements seemed to possess a weakened membrane as suggested by their increased susceptibility to osmotic lysis and their flattened shape. Irregular protuberances, which may be associated with formation of elementary corpuscles or degeneration of membrane, were found on large bodies in stationary phase. Replication in broth appeared to occur by single budding of large bodies or binary fission of granules measuring 0.7 μm or more in diameter. L-Phase colonies of group D streptococci, strains F24 and Z30, and group A streptococci, strains ADA, AED, and GL8, were differentiated by morphology when grown on Millipore membrane filters pressed upon uniform agar medium. Both group and strain variation were observed.

scholarly journals Comparison of Gelman and Millipore Membrane Filters for Enumerating Fecal Coliform Bacteria

1973 ◽  
Vol 26 (3) ◽  
pp. 332-336 ◽  
Author(s):  
William G. Presswood ◽  
Lynn R. Brown
Keyword(s):  
Fecal Coliform ◽  
Coliform Bacteria ◽  
Fecal Coliform Bacteria ◽  
Membrane Filters ◽  
Millipore Membrane

scholarly journals Statistical Analysis of the Recovery of Coliform Organisms on Gelman and Millipore Membrane Filters

1974 ◽  
Vol 28 (4) ◽  
pp. 605-607
Author(s):  
David J. Schaeffer ◽  
Maxine C. Long ◽  
Konanur G. Janardan
Keyword(s):  
Statistical Analysis ◽  
Membrane Filters ◽  
Millipore Membrane

scholarly journals Enrichment of extracellular vesicles using Millipore membrane filters

2015 ◽  
Author(s):  
Roger P. Alexander ◽  
Louise C. Laurent ◽  
Roger P. Alexander
Keyword(s):  
Extracellular Vesicles ◽  
Membrane Filters ◽  
Millipore Membrane

Correlations of the Protozoa, Cryptosporidium and Giardia, with Water Quality Variables in a Watershed

1988 ◽  
Vol 20 (11-12) ◽  
pp. 271-276 ◽  
Author(s):  
Joan B. Rose ◽  
Hamid Darbin ◽  
Charles P. Gerba
Keyword(s):  
Fecal Coliform ◽  
Fluorescent Microscopy ◽  
Correlation Coefficients ◽  
Fecal Coliforms ◽  
Indicator Organisms ◽  
Membrane Filters ◽  
Bacterial Indicator ◽  
Indirect Immunofluorescent Assay ◽  
Indirect Immunofluorescent ◽  
Enteric Protozoa

A watershed in the western United States was surveyed biweekly for a year for the protozoa Cryptosporidium and Giardia. Parasite samples were collected using filtration of 200-1000 L of water, eluted by washing the filter, concentrated and clarified with density gradients. Oocyst and cysts were detected using monoclonal antibodies, in a direct or indirect immunofluorescent assay. The organisms were enumerated on membrane filters using epi-fluorescent microscopy. Number of organisms per liter of water were then calculated. Grab samples were analyzed for total and fecal coliform concentrations as well as turbidities. After log10(y+1.0) transformations, Pearson's correlation coefficients were derived for each variable against all others. From 39 samples, Cryptosporidium and Giardia were detected in 20 and 12 of the samples, respectively, and oocyst concentrations were approximately 10 times higher than cyst levels. The river downstream from the lake ran through an area concentrated with cattle pasture and had the greater numbers of both parasites (1.09 oocysts and 0.22 cysts/L). The lake, although receiving sewage effluents, may have also had lower levels (0.58 oocysts and 0.08 cysts/L) because of dilution and sedimentation. Coliform levels ranged from 0.12 to 75 CFU/l00ml while fecal coliform levels were slightly lower. Turbidity averaged 55 NTU at the river and 48 NTU at the lake. Correlation coefficients were derived for all variables. Giardia concentrations were significantly correlated to Cryptosporidium concentrations with an r value of 0.778, significant at the 99% confidence level. No other correlations were observed between Giardia and total or fecal coliforms or turbidities nor between Cryptosporidium levels and total or fecal coliforms or turbidities. Neither bacterial indicator organisms nor turbidity are reliable predictors for the absence of enteric protozoa in the study watershed.

An evaluation of three agents for eluting adsorbed enterovirus from millipore membrane filters

1971 ◽  
Vol 17 (11) ◽  
pp. 1351-1355 ◽  
Author(s):  
Jack Konowalchuk ◽  
Joan I. Speirs
Keyword(s):  
Yeast Extract ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Cellulose Nitrate ◽  
Membrane Filters ◽  
Beef Extract ◽  
Fetal Bovine ◽  
Millipore Membrane ◽  
High Degree

Several concentrations of yeast extract, beef extract, and fetal bovine serum were compared for ability to elute adsorbed coxsackievirus B5 and poliovirus 1 from HA cellulose nitrate Millipore membrane filters. Elution improved with increases in concentration of eluting agent and volume of eluent. The eluent of choice was undiluted fetal bovine serum because it was compatible with cells and showed a high degree of elution. A 5-ml serum eluate resulted in the recovery of 80–90% of the virus adsorbed by a filter from an infected buffered saline sample.

Enrichment of extracellular vesicles using Millipore membrane filters followed by isolation of exosomal RNA from serum or plasma using the Qiagen miRNeasy Micro kit

2017 ◽  
Author(s):  
Roger P. Alexander ◽  
Leonora Balaj ◽  
Justyna Filant ◽  
Parham Nejad ◽  
Anu Paul ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Membrane Filters ◽  
Millipore Membrane ◽  
Qiagen Mirneasy

scholarly journals Comparison of Gelman and Millipore Membrane Filters for Enumerating Fecal Coliform Bacteria

1973 ◽  
Vol 26 (3) ◽  
pp. 332-336
Author(s):  
William G. Presswood ◽  
Lynn R. Brown
Keyword(s):  
Fecal Coliform ◽  
Coliform Bacteria ◽  
Fecal Coliform Bacteria ◽  
Membrane Filters ◽  
Millipore Membrane
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