TMKS8A, an antibacterial and cytotoxic chlorinated α-lapachone, from a sea slug-derived actinomycete of the genus Streptomyces

2021 ◽  
Author(s):  
Zhiwei Zhang ◽  
Mada Triandala Sibero ◽  
Akiho Kai ◽  
Keisuke Fukaya ◽  
Daisuke Urabe ◽  
...  
Keyword(s):  
Genus Streptomyces ◽  
Sea Slug

Complete genome of mangrove-derived anti-MRSA streptomycete, Streptomyces pluripotens MUSC 135T

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Hooi-Leng Ser ◽  
Kok-Gan Chan ◽  
Wen-Si Tan ◽  
Wai-Fong Yin ◽  
Bey-Hing Goh ◽  
...  
Keyword(s):  
Complete Genome ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Gene Clusters ◽  
Salmonella Typhi ◽  
Bacteriocin Production ◽  
Trna Genes ◽  
Genus Streptomyces ◽  
Mining Tool ◽  
Bioactive Potential ◽  
Effective Drugs

Microorganisms serve as attractive resources, owing to their ability to synthesize structurally-diverse substanceswith various bioactivities. Within the Bacteria domain, members of the genus Streptomyces have demonstrated remarkableability to produce clinically useful, secondary metabolites such as anticancer, antioxidants, antivirals and antibacterials.Streptomyces pluripotens MUSC 135T was isolated as novel strain from mangrove forest in Malaysia. This strain exhibitedbroad spectrum bacteriocin against several pathogens including methicillin-resistant Staphylococcus aureus (MRSA) strainATCC BAA-44, Salmonella typhi ATCC 19430T and Aeromonas hydrophila ATCC 7966T. Thus, the strain was selected forwhole genome sequencing as an attempt to explore its bioactive potential. Here we report the first complete genome of S.pluripotens MUSC 135T genome which comprise of 7.35 Mbp with G+C content of 69.9 %. A total of 6,404 open readingframes (ORFs) were predicted, along with 18 rRNA and 69 tRNA genes. Using bacteriocin mining tool, BAGEL detectedeights gene clusters associated with bacteriocin production including lanthipeptides and linear azol(in)e-containing peptides(LAPs). Members of Streptomyces have contributed greatly towards improving lives, particularly against deadly infectionsand chronic diseases. The availability of S. pluripotens MUSC 135T genome sequence has opened new window for drugdiscovery, particularly for effective drugs against harmful pathogens such as MRSA and certainly deserves further detailedstudy.

scholarly journals Outbreak of a neurotoxic side-gilled sea slug (Pleurobranchaea sp.) in Argentinian coasts

2015 ◽  
Vol 42 (1) ◽  
pp. 51-56 ◽  
Author(s):  
NE Farias ◽  
S Obenat ◽  
AB Goya
Keyword(s):  
Sea Slug

scholarly journals A Survey of Genetic Variation in Streptomyces Isolates Causing Potato Common Scab in the United States

2006 ◽  
Vol 96 (12) ◽  
pp. 1363-1371 ◽  
Author(s):  
Leslie A. Wanner
Keyword(s):  
United States ◽  
Genetic Variation ◽  
Common Scab ◽  
Disease Incidence ◽  
The United States ◽  
Rrna Gene ◽  
Population Genetic Study ◽  
Variable Regions ◽  
Genus Streptomyces ◽  
Characteristic Features

Common scab is a serious disease of potatoes and other root and tuber crops, affecting crop quality and market value. The disease is caused by gram positive soil bacteria in the genus Streptomyces. Disease incidence and severity vary in different locations and years; this is due in part to variation in the environment (weather) and genetic variation in potato cultivars. Little information is available on the contribution of genetic variation by the pathogen. To examine genetic diversity in different locations within the United States, streptomycetes were isolated from lesions on field-grown potatoes from six states. Isolates were classified into species based on sequence of variable regions in the 16s rRNA gene. The presence of genes associated with the recently described S. turgidiscabies pathogenicity island (PAI) was also determined. About half of the isolates belonged to S. scabies or S. europaeiscabiei based on 16s rDNA sequence, and had characteristic features of the PAI. They were found in all six states, and were pathogenic on potato and radish. The remaining isolates included pathogens and nonpathogens. They were varied in appearance, and represent several species, including one pathogenic species not previously reported. Some pathogenic isolates lacked one or more genes characteristic of the PAI, although all had genes for biosynthesis of the pathogenicity determinant thaxtomin. In this relatively small survey, regional differences in scab-causing streptomycetes were seen. This report furnishes tools and baseline data for population genetic study of scab-causing streptomycetes in the United States.

scholarly journals An Actinobacterial Isolate, Streptomyces sp. YX44, Produces Broad-Spectrum Antibiotics That Strongly Inhibit Staphylococcus aureus

2021 ◽  
Vol 9 (3) ◽  
pp. 630
Author(s):  
Tien-Lin Chang ◽  
Tzu-Wen Huang ◽  
Ying-Xuan Wang ◽  
Chang-Pan Liu ◽  
Ralph Kirby ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Fermentation Broth ◽  
Multidrug Resistant ◽  
Size Exclusion ◽  
Significant Activity ◽  
Health Food ◽  
Strong Activity ◽  
Genus Streptomyces ◽  
Streptomyces Sp ◽  
Good Solubility

The need for new antibiotics is increasing due to their overuse, and antibiotic resistance has become one of the major threats worldwide to public health, food safety, and clinical treatment. In this study, we describe an actinobacterial isolate, YX44, which belongs to the genus Streptomyces. This Streptomyces was isolated from a drinking pipe located in Osaka, Japan, and has the ability to inhibit Gram-positive bacteria, Gram-negative bacteria, and various fungi. YX44 fermentation broth shows strong activity against Escherichia coli and Staphylococcus aureus, as well as also inhibiting clinical isolates of multidrug-resistant Staphylococcus aureus. The YX44 antibacterial substances in the broth are relatively heat-stable, show high stability from the pH range 1 to 11, and have good solubility in both organic and non-organic solvents. Size-exclusion chromatography revealed that the YX44 antibacterial compounds are less than 1000 Da in size. LC-MS was able to identify three possible candidate molecules with molecular weights of 308, 365, 460, and 653 g/mol; none of these sizes correspond to any well-known antibiotics. Our results show that Streptomyces sp. YX44 seems to produce a number of novel antibiotics with high pH stability and good solubility that have significant activity against S. aureus, including multidrug-resistant strains.

scholarly journals cAMP, Ca2+, pHi, and NO Regulate H-like Cation Channels That Underlie Feeding and Locomotion in the Predatory Sea Slug Pleurobranchaea californica

2018 ◽  
Vol 9 (8) ◽  
pp. 1986-1993
Author(s):  
Daniel J. Green ◽  
Rong-Chi Huang ◽  
Leland Sudlow ◽  
Nathan Hatcher ◽  
Kurt Potgieter ◽  
...  
Keyword(s):  
Cation Channels ◽  
Sea Slug ◽  
Pleurobranchaea Californica

scholarly journals Angucycline-like Aromatic Polyketide from a Novel Streptomyces Species Reveals Freshwater Snail Physa acuta as Underexplored Reservoir for Antibiotic-Producing Actinomycetes

Antibiotics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 22
Author(s):  
Nasim Safaei ◽  
Yvonne Mast ◽  
Michael Steinert ◽  
Katharina Huber ◽  
Boyke Bunk ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Genome Mining ◽  
Freshwater Snail ◽  
Whole Genome Sequence ◽  
Phylogenomic Analysis ◽  
Ionization Mass ◽  
Gram Positive ◽  
Streptomyces Species ◽  
Genus Streptomyces ◽  
Physa Acuta

Antibiotic producers have mainly been isolated from soil, which often has led to the rediscovery of known compounds. In this study, we identified the freshwater snail Physa acuta as an unexplored source for new antibiotic producers. The bacterial diversity associated with the snail was characterized by a metagenomic approach using cultivation-independent high-throughput sequencing. Although Actinobacteria represented only 2% of the bacterial community, the focus was laid on the isolation of the genus Streptomyces due to its potential to produce antibiotics. Three Streptomyces strains (7NS1, 7NS2 and 7NS3) were isolated from P. acuta, and the antimicrobial activity of the crude extracts were tested against a selection of Gram-positive and Gram-negative bacteria and fungi. 7NS3 showed the strongest activity against Gram-positive bacteria and, thus, was selected for genome sequencing and a phylogenomic analysis. 7NS3 represents a novel Streptomyces species, which was deposited as Streptomyces sp. DSM 110735 at the Leibniz Institute-German Collection of Microorganisms and Cell Cultures (DSMZ). Bioassay-guided high-performance liquid chromatography (HPLC) and high-resolution electrospray ionization-mass spectrometry (HR-ESI-MS) analyses of crude extract fractions resulted in the detection of four compounds, one of which matched the compound characteristics of emycin A, an angucycline-like aromatic polyketide. Genome mining studies based on the whole-genome sequence of 7NS3 resulted in the identification of a gene cluster potentially coding for emycin A biosynthesis. Our study demonstrates that freshwater snails like P. acuta can represent promising reservoirs for the isolation of new antibiotic-producing actinobacterial species.

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