scholarly journals Simulation of hypoxia of myocardial cells in microfluidic systems

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Anna Kobuszewska ◽  
Elżbieta Jastrzębska ◽  
Kamil Żukowski ◽  
Zbigniew Brzózka
Keyword(s):  
Fluorescent Dyes ◽  
Microfluidic System ◽  
Cardiac Cells ◽  
Human Cardiomyocytes ◽  
New Methods ◽  
Biochemical Simulation ◽  
Carbonyl Cyanide ◽  
Myocardial Hypoxia ◽  
Biochemical Approach ◽  
Methods Of Treatment

Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.

scholarly journals Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 131
Author(s):  
Anna Kobuszewska ◽  
Dominik Kolodziejek ◽  
Michal Wojasinski ◽  
Tomasz Ciach ◽  
Zbigniew Brzozka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Metabolic Activity ◽  
Microfluidic System ◽  
Cardiac Cells ◽  
Effective Solution ◽  
Mitochondrial Oxidative Phosphorylation ◽  
Human Cardiomyocytes ◽  
Bioluminescence Assay ◽  
Nanofiber Mats

Regenerative medicine and stem cells could prove to be an effective solution to the problem of treating heart failure caused by ischemic heart disease. However, further studies on the understanding of the processes which occur during the regeneration of damaged tissue are needed. Microfluidic systems, which provide conditions similar to in vivo, could be useful tools for the development of new therapies using stem cells. We investigated how mesenchymal stem cells (MSCs) affect the metabolic activity of cardiac cells (rat cardiomyoblasts and human cardiomyocytes) incubated with a potent uncoupler of mitochondrial oxidative phosphorylation under microfluidic conditions. A cyanide p-trifluoromethoxyphenylhydrazone (FCCP) was used to mimic disfunctions of mitochondria of cardiac cells. The study was performed in a microfluidic system integrated with nanofiber mats made of poly-l-lactid acid (PLLA) or polyurethane (PU). The microsystem geometry allows four different cell cultures to be conducted under different conditions (which we called: normal, abnormal—as both a mono- and co-culture). Metabolic activity of the cells, based on the bioluminescence assay, was assessed in the culture’s performed in the microsystem. It was proved that stem cells increased metabolic activity of cardiac cells maintained with FCCP.

scholarly journals Systematics Of Paramphistomatosis, Methods Of Diagnosis, Epizootology, Pathogenesis, Origin Of The Disease And New Methods Of Treatment

2020 ◽  
Vol 2 (06) ◽  
pp. 1-6
Author(s):  
Avezimbetov Sh. ◽  
◽  
Taylakov T.I ◽  
Keyword(s):  
Domestic Animals ◽  
Anatomical Changes ◽  
New Methods ◽  
Methods Of Treatment

This article describes paramphistomatosis of large and small horned domestic animals, its systematics, and methods of helminthocaprological examination, paramphistomatosis found in the large and retinal abdomen of dead and forcibly slaughtered animals, stages of ovulation in mollusks, pathogenesis, pathological and anatomical changes and treatment reported.

scholarly journals ETHICAL DIMENSIONS OF WILLINGNESS TO PAY FOR MEDICAL CARE IN BULGARIA

2018 ◽  
Vol 6 (1) ◽  
pp. 516-522
Author(s):  
Kalina Peycheva ◽  
Mariela Deliverska
Keyword(s):  
Higher Education ◽  
Medical Care ◽  
Education Level ◽  
Medical Services ◽  
Medical Professionals ◽  
Chi Square ◽  
New Methods ◽  
Gender And Education ◽  
Anonymous Questionnaire ◽  
Methods Of Treatment

Regardless of what both patients and medical professionals might think, nowadays there is no free medicine. The need of changing the pattern is emphasized and people should become more responsible for their own health. The aim is to find a connection between the trust in GPs, prophylactic check-ups, new methods of treatment and the willingness of patients to pay for the received medical services. Material and Method: A questionnaire was prepared for the purposes of the study. The methods utilized were a direct individual anonymous questionnaire, statistical – descriptive, analytical (Chi-square). The answers were examined and statistically processed according to age, gender and education level of the participants. Results: 1. The results regarding the trust in GP is very unconvincing – only 14,5 % believe in their GP. 2. The percentage of believers in prophylactic check-ups is high - 57,9%.  3.The percentage of those who believe in the new methods and means for treatment is high, over 80%, while no difference is found with respect to the patients’ education level. 4. The patients often (86%) pay for the treatment of a specialist. 5. People with higher education more readily pay for medical care. Conclusions: 1. The lack of trust in GP combined with the strong belief in prophylactic check - ups and the new methods for diagnostic and treatment of diseases lead to higher expectations of patients towards the medical services and their readiness to pay for these services. 2. The patients indicate readiness to pay for medical services which is a part of the patients’ readiness to take care for their own health.

THE INFLUENCE OF CLINICAL AND LABORATORY FACTORS ON THE DEVELOPMENT OF HEMODYNAMIC COMPLICATIONS DURING ANESTHESIA IN CARDIAC SURGERY

2021 ◽  
Vol 19 (4) ◽  
pp. 367-375
Author(s):  
R. E. Yakubtsevich ◽  
◽  
K. O. Kratkou ◽  
Keyword(s):  
Cardiac Surgery ◽  
Cardiovascular Diseases ◽  
Endothelial Dysfunction ◽  
Postoperative Period ◽  
Modern Medicine ◽  
New Methods ◽  
Laboratory Markers ◽  
Tnf Α ◽  
Cellular Hypoxia ◽  
Methods Of Treatment

Background. Currently, cardioanesthesiology is one of the most actively developing areas of modern medicine. Thanks to new methods of treatment, the contingent of patients for whom it became possible to undergo cardiac surgery has significantly expanded. The main problems that lead to hemodynamic complications are endothelial dysfunction and cellular hypoxia. Purpose. To present data on the influence of clinical and laboratory factors of endothelial dysfunction and cellular hypoxia on the development of hemodynamic complications during anesthesia in cardiac surgery. Material and methods. The review and analysis of literature data from 49 sources is presented. Results. The laboratory markers of endothelial dysfunction leading to the development of major hemodynamic complications in cardiovascular diseases are MPC-1, CRP, NO, TNF-α, IL-6, homocysteine. Conclusion. The data obtained indicate a significant effect of cell markers (MPC-1, CRP, NO, TNF-A, IL-6, homocysteine) as well as clinical and laboratory factors of endothelial dysfunction not only on the development of major diseases of the cardiovascular system, but also on their complications. An early study of these markers can improve anesthesia during cardiac surgery as well as reduce complications in the postoperative period.

The Unified Protocol

2017 ◽  
pp. 291-302
Author(s):  
Clair Cassiello-Robbins ◽  
Heather Murray Latin ◽  
Shannon Sauer-Zavala
Keyword(s):  
Emotional Disorders ◽  
Individual Treatment ◽  
Compelling Evidence ◽  
Future Directions ◽  
Treatment Delivery ◽  
Transdiagnostic Treatment ◽  
New Methods ◽  
Unified Protocol ◽  
Effective Care ◽  
Methods Of Treatment

Previous chapters in this book have been dedicated to exploring the use of the Unified Protocol for the Transdiagnostic Treatment of Emotional Disorders (UP) with a variety of emotional disorders, and those chapters present compelling evidence for the efficacy of the UP across the range of these disorders. This evidence has now inspired further research efforts to better understand the contribution of individual treatment components, as well as to evaluate new methods of treatment delivery with the goal of furthering dissemination. The purpose of this chapter is to describe future directions and projects related to the UP, including dismantling studies, dissemination efforts, and the development of an Internet-delivered form of the intervention. Further, we discuss how these projects will enhance our understanding of the UP and how these endeavors will further a clinician’s ability to provide efficient, effective care.

scholarly journals Production and Application of Multicistronic Constructs for Various Human Disease Therapies

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 580 ◽  
Author(s):  
Alisa A. Shaimardanova ◽  
Kristina V. Kitaeva ◽  
Ilmira I. Abdrakhmanova ◽  
Vladislav M. Chernov ◽  
Catrin S. Rutland ◽  
...  
Keyword(s):  
Human Diseases ◽  
Cellular Biology ◽  
Entry Site ◽  
Internal Ribosome Entry ◽  
New Methods ◽  
In Vivo Experiments ◽  
Mutant Genes ◽  
Methods Of Treatment

The development of multicistronic vectors has opened up new opportunities to address the fundamental issues of molecular and cellular biology related to the need for the simultaneous delivery and joint expression of several genes. To date, the examples of the successful use of multicistronic vectors have been described for the development of new methods of treatment of various human diseases, including cardiovascular, oncological, metabolic, autoimmune, and neurodegenerative disorders. The safety and effectiveness of the joint delivery of therapeutic genes in multicistronic vectors based on the internal ribosome entry site (IRES) and self-cleaving 2A peptides have been shown in both in vitro and in vivo experiments as well as in clinical trials. Co-expression of several genes in one vector has also been used to create animal models of various inherited diseases which are caused by mutations in several genes. Multicistronic vectors provide expression of all mutant genes, which allows the most complete mimicking disease pathogenesis. This review comprehensively discusses multicistronic vectors based on IRES nucleotide sequence and self-cleaving 2A peptides, including its features and possible application for the treatment and modeling of various human diseases.

scholarly journals Phenotypic Screen with the Human Secretome Identifies FGF16 as Inducing Proliferation of iPSC-Derived Cardiac Progenitor Cells

2019 ◽  
Vol 20 (23) ◽  
pp. 6037 ◽  
Author(s):  
Karin Jennbacken ◽  
Fredrik Wågberg ◽  
Ulla Karlsson ◽  
Jerry Eriksson ◽  
Lisa Magnusson ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cardiac Fibroblasts ◽  
Cardiac Regeneration ◽  
Cardiac Cells ◽  
Mouse Heart ◽  
Cardiac Progenitor Cells ◽  
High Sequence Identity ◽  
Paracrine Factors ◽  
Human Cardiomyocytes ◽  
Phenotypic Screen

Paracrine factors can induce cardiac regeneration and repair post myocardial infarction by stimulating proliferation of cardiac cells and inducing the anti-fibrotic, antiapoptotic, and immunomodulatory effects of angiogenesis. Here, we screened a human secretome library, consisting of 923 growth factors, cytokines, and proteins with unknown function, in a phenotypic screen with human cardiac progenitor cells. The primary readout in the screen was proliferation measured by nuclear count. From this screen, we identified FGF1, FGF4, FGF9, FGF16, FGF18, and seven additional proteins that induce proliferation of cardiac progenitor cells. FGF9 and FGF16 belong to the same FGF subfamily, share high sequence identity, and are described to have similar receptor preferences. Interestingly, FGF16 was shown to be specific for proliferation of cardiac progenitor cells, whereas FGF9 also proliferated human cardiac fibroblasts. Biosensor analysis of receptor preferences and quantification of receptor abundances suggested that FGF16 and FGF9 bind to different FGF receptors on the cardiac progenitor cells and cardiac fibroblasts. FGF16 also proliferated naïve cardiac progenitor cells isolated from mouse heart and human cardiomyocytes derived from induced pluripotent cells. Taken together, the data suggest that FGF16 could be a suitable paracrine factor to induce cardiac regeneration and repair.

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