scholarly journals A rapid and highly sensitive biomarker detection platform based on a temperature-responsive liposome-linked immunosorbent assay

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Runkai Hu ◽  
Keitaro Sou ◽  
Shinji Takeoka
Keyword(s):  
Immunosorbent Assay ◽  
Limit Of Detection ◽  
Specific Antigen ◽  
Fluorescence Emission ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Temperature Responsive ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Abstract The enzyme-linked immunosorbent assay (ELISA) is widely used in various fields to detect specific biomarkers. However, ELISA tests have limited detection sensitivity (≥ 1 pM), which is insufficiently sensitive for the detection of small amounts of biomarkers in the early stages of disease or infection. Herein, a method for the rapid and highly sensitive detection of specific antigens, using temperature-responsive liposomes (TLip) containing a squaraine dye that exhibits fluorescence at the phase transition temperature of the liposomes, was developed. A proof-of-concept study using biotinylated TLip and a streptavidin-immobilized microwell plate showed that the TLip bound to the plate via specific molecular recognition could be distinguished from unbound TLip within 1 min because of the difference in the heating time required for the fluorescence emission of TLip. This system could be used to detect prostate specific antigen (PSA) based on a sandwich immunosorbent assay using detection and capture antibodies, in which the limit of detection was as low as 27.6 ag/mL in a 100-μL PSA solution, 0.97 aM in terms of molar concentration. The present temperature-responsive liposome-linked immunosorbent assay provides an advanced platform for the rapid and highly sensitive detection of biomarkers for use in diagnosis and biological inspections.

Capillary-based enzyme-linked immunosorbent assay for highly sensitive detection of thrombin-cleaved osteopontin in plasma

2013 ◽  
Vol 440 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Shun-ichi Funano ◽  
Terence G. Henares ◽  
Mie Kurata ◽  
Kenji Sueyoshi ◽  
Tatsuro Endo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Fluorescence ELISA based on CAT-regulated fluorescence quenching of CdTe QDs for sensitive detection of FB1

2018 ◽  
Vol 10 (48) ◽  
pp. 5797-5802 ◽  
Author(s):  
Tianying Lu ◽  
Shengnan Zhan ◽  
Yaofeng Zhou ◽  
Xirui Chen ◽  
Xiaolin Huang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Quenching ◽  
Immunosorbent Assay ◽  
Cdte Quantum Dots ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mercaptopropionic Acid ◽  
Cdte Qds ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A competitive fluorescence enzyme-linked immunosorbent assay (cFELISA) was developed for the highly sensitive detection of fumonisin B1 (FB1) based on the catalase (CAT)-regulated fluorescence quenching of mercaptopropionic acid-modified CdTe quantum dots (MPA-QDs).

A supramolecular nanobiological hybrid as a PET sensor for bacterial DNA isolated from Streptomyces sanglieri

The Analyst ◽  
2014 ◽  
Vol 139 (24) ◽  
pp. 6502-6510 ◽  
Author(s):  
Sudesna Chakravarty ◽  
Dilip Saikia ◽  
Priyanka Sharma ◽  
Nirab Chandra Adhikary ◽  
Debajit Thakur ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Sensitive Detection ◽  
Bacterial Dna ◽  
Turn On ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A ‘turn on–off–on’ sensor for highly sensitive detection of ds DNA with an excellent ‘limit of detection’ is reported.

scholarly journals Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 378
Author(s):  
Jinfeng Quan ◽  
Yihan Wang ◽  
Jialei Zhang ◽  
Kejing Huang ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Dna Sequences ◽  
Breast Cancer Cells ◽  
Limit Of Detection ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Cruciform Dna ◽  
Mcf 7

Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10 − 1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

Highly sensitive detection of nitrite by using gold nanoparticle-decorated α-Fe2O3 nanorod arrays as self-supporting photo-electrodes

2019 ◽  
Vol 6 (6) ◽  
pp. 1432-1441 ◽  
Author(s):  
Shenghong Kang ◽  
Haimin Zhang ◽  
Guozhong Wang ◽  
Yunxia Zhang ◽  
Huijun Zhao ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Nanorod Arrays ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gold nanoparticle (Au NP)-decorated-Fe2O3 nanorod arrays (AuNPs-Fe2O3) as a photoelectrode are applied to the detection of nitrite solution with a low limit of detection and high sensitivity.

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