scholarly journals Graphene oxide and its derivatives as promising In-vitro bio-imaging platforms

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yasaman Esmaeili ◽  
Elham Bidram ◽  
Ali Zarrabi ◽  
Abbas Amini ◽  
Chun Cheng
Keyword(s):  
Graphene Oxide ◽  
Optical Sensors ◽  
Near Infrared ◽  
Fluorescent Microscopy ◽  
Photoluminescence Property ◽  
Infrared Range ◽  
Chemical Structures ◽  
Bio Imaging ◽  
The Impact

Abstract Intrinsic fluorescence and versatile optical properties of Graphene Oxide (GO) in visible and near-infrared range introduce this nanomaterial as a promising candidate for numerous clinical applications for early-diagnose of diseases. Despite recent progresses in the impact of major features of GO on the photoluminescence properties of GO, their modifications have not yet systematically understood. Here, to study the modification effects on the fluorescence behavior, poly ethylene glycol (PEG) polymer, metal nanoparticles (Au and Fe3O4) and folic acid (FA) molecules were used to functionalize the GO surface. The fluorescence performances in different environments (water, DMEM cell media and phosphate buffer with two different pH values) were assessed through fluorescence spectroscopy and fluorescent microscopy, while Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) and Scanning electron microscopy (SEM) were utilized to evaluate the modifications of chemical structures. The modification of GO with desired molecules improved the photoluminescence property. The synthesized platforms of GO-PEG, GO-PEG-Au, GO-PEG-Fe3O4 and GO-PEG-FA illustrated emissions in three main fluorescence regions (blue, green and red), suitable for tracing and bio-imaging purposes. Considering MTT results, these platforms potentially positioned themselves as non-invasive optical sensors for the diagnosis alternatives of traditional imaging agents.

scholarly journals Uniform Spheres of α-NaYF4:RE3+ (RE=Eu, Tb, Ce, Er, and Tm): Template-Free Synthesis, Multi-Color Photoluminescence, and Their Application in Cellular Imaging

Crystals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 119
Author(s):  
Xiaofeng Fan ◽  
Laiqin Gu ◽  
Yiling Hu ◽  
Qi Zhu
Keyword(s):  
Near Infrared ◽  
Infrared Emission ◽  
Particle Sizes ◽  
Hydrothermal Processing ◽  
High Brightness ◽  
Luminescent Probes ◽  
Bio Imaging ◽  
Template Free ◽  
Near Infrared Emission

Uniformly dispersed luminescent probes with a high brightness and high resolution are desired in bio imaging fields. Here, ~100 nm sized and well-dispersed spheres of RE3+ doped α-NaYF4 (rare earth (RE) = Eu, Tb, Ce, Er, and Tm) have been facile synthesized through hydrothermal processing in the absence of a template, followed by a proper annealing. The processing window of the cubic structured spheres is wide, because the hydrothermal products are independent of the processing conditions, including reaction time and temperature. The original morphology and crystal structure can be well retained with a calcination temperature up to 600 °C. However, calcination gives rise to a reduction of particle sizes, as a result of the crystallite growth and densification. Under ultraviolet radiation, α-NaYF4:RE3+ spheres show characteristic f-f emissions of RE3+ (RE = Eu, Tb, Ce, Er, and Tm), and exhibit orange red, green, ultraviolet (UV), blue green, and blue emissions, respectively. Mainly because of the near-infrared emission at ~697 nm (5D0→7F4 transitions of Eu3+), the successful imaging of macrophages was achieved by NH2-NaYF4:Eu3+ probes, indicating their excellent imaging capacity for cells in vitro.

scholarly journals Author Correction: Graphene oxide and its derivatives as promising In-vitro bio-imaging platforms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yasaman Esmaeili ◽  
Elham Bidram ◽  
Ali Zarrabi ◽  
Abbas Amini ◽  
Chun Cheng
Keyword(s):  
Graphene Oxide ◽  
Bio Imaging

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Photothermal-assisted antibacterial application of GO-Ag against clinical isolated MDR E. coli

2019 ◽  
Author(s):  
Yuqing Chen ◽  
Wei Wu ◽  
Zeqiao Xu ◽  
Cheng Jiang ◽  
Shuang Han ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Near Infrared ◽  
Antibacterial Effect ◽  
Luria Bertani ◽  
Fluorescent Imaging ◽  
Photothermal Effect ◽  
Antibacterial Efficiency ◽  
E Coli ◽  
Morphology Characterization

Abstract Background: Treatment of multidrug-resistant (MDR) bacterial infection is a great challenge in public health. Herein, we provide a solution to this problem with the use of graphene oxide-silver (GO-Ag) nanocomposites as anti-bacterial agent. Methods: Following established protocols, silver nanoparticles were grown on graphene oxide sheets. Then, a series of in-vitro studies were conducted to validate the antibacterial efficiency of the GO-Ag nanocomposites against clinical MDR Escherichia coli (E. coli) strains. Firstly, minimum inhibitory concentrations (MICs) of different antimicrobials were tested against MDR E. Coli strains. Then, bacteria viability assessments were conducted with different nanomaterials in Luria-Bertani (LB) broth. Afterwards, photothermal irradiation was conducted on MDR E. coli with lower GO-Ag concentration. At last, fluorescent imaging and morphology characterization using scanning electron microscope (SEM) were done to find the possible cause of antibacterial effect. Results: GO-Ag nanocomposites showed the highest antibacterial efficiency among tested antimicrobials. Synergetic antibacterial effect was observed in GO-Ag nanocomposites treated group. The remained bacteria viabilities were 4.4% and 4.1% respectively for different bacteria strains with GO-Ag concentration at 14.0 µg mL-1. In addition, GO-Ag nanocomposites have strong absorption in the near-infrared field and can convert the electromagnetic energy to heat. With the use of this photothermal effect, effective sterilization could be achieved using GO-Ag nanocomposites concentration as low as 7.0 µg mL-1. Fluorescent imaging and morphology characterization were used to analyze bacteria living status, which uncovered that bacteria integrity was disrupted after GO-Ag nanocomposites treatment. Conclusions: GO-Ag nanocomposites are proved to be efficient antibacterial agent against multi-drug resistant E. coli. Their strong antibacterial effect arises from inherent antibacterial property and photothermal effect that provides aid for bacteria killing.

scholarly journals Tungsten disulfide-based nanocomposites for photothermal therapy

2019 ◽  
Vol 10 ◽  
pp. 811-822 ◽  
Author(s):  
Tzuriel Levin ◽  
Hagit Sade ◽  
Rina Ben-Shabbat Binyamini ◽  
Maayan Pour ◽  
Iftach Nachman ◽  
...  
Keyword(s):  
Photothermal Therapy ◽  
Near Infrared ◽  
Transition Metal Dichalcogenides ◽  
Tungsten Disulfide ◽  
Ceric Ammonium Nitrate ◽  
Infrared Range ◽  
Metal Dichalcogenides ◽  
Current Article ◽  
Functionalized Nanotubes

Nanostructures of transition-metal dichalcogenides (TMDC) have raised scientific interest in the last few decades. Tungsten disulfide (WS2) nanotubes and nanoparticles are among the most extensively studied members in this group, and are used for, e.g., polymer reinforcement, lubrication and electronic devices. Their biocompatibility and low toxicity make them suitable for medical and biological applications. One potential application is photothermal therapy (PTT), a method for the targeted treatment of cancer, in which a light-responsive material is irradiated with a laser in the near-infrared range. In the current article we present WS2 nanotubes functionalized with previously reported ceric ammonium nitrate–maghemite (CAN-mag) nanoparticles, used for PTT. Functionalization of the nanotubes with CAN-mag nanoparticles resulted in a magnetic nanocomposite. When tested in vitro with two types of cancer cells, the functionalized nanotubes showed a better PTT activity compared to non-functionalized nanotubes, as well as reduced aggregation and the ability to add a second-step functionality. This ability is demonstrated here with two polymers grafted onto the nanocomposite surface, and other functionalities could be additional cancer therapy agents for achieving increased therapeutic activity.

Multifunctional graphene oxide for bioimaging: emphasis on biological research

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Do Won Hwang ◽  
Byung Hee Hong ◽  
Dong Soo Lee
Keyword(s):  
Graphene Oxide ◽  
Targeted Delivery ◽  
Near Infrared ◽  
Biological Research ◽  
Natural Interaction ◽  
Drug Delivery Carrier ◽  
Wide Range ◽  
Surface Enhanced Raman

AbstractGraphene oxide (GO) nanomaterials offer a wide range of bioimaging applicability. Almost complete quenching ability of fluorescence by GO and natural interaction of GO with single stranded nucleic acid made GO a useful and intriguing multifunctional nanoplatform both as a biosensor for in vitro microplate diagnostics and as a drug delivery carrier for targeted delivery. GO’s large surface area and strong near infrared absorbance contribute to enhancement of a therapeutic effect with abundant loading of drugs for possible photothermal and photodynamic therapy. Bioimaging capability of GO made it a good theranostic tool, while enabling tracing in vivo pharmacokinetics during concurrent treatment. Fluorescence, either signal on or off, Raman and surface-enhanced Raman scattering (SERs), photoacoustic, and radionuclide imaging modalities can be used for theranostic purposes using GO nanomaterials. In this review, we highlight current applications of GO for bioimaging that are classified into in vitro microplate, in vitro cellular and in vivo bioimaging.

scholarly journals Commercial polycarbonate track-etched membranes as substrates for low-cost optical sensors

2019 ◽  
Vol 10 ◽  
pp. 677-683 ◽  
Author(s):  
Paula Martínez-Pérez ◽  
Jaime García-Rupérez
Keyword(s):  
Optical Sensors ◽  
Mesoporous Material ◽  
Near Infrared ◽  
Low Cost ◽  
Ethanol Solution ◽  
Infrared Range ◽  
Refractive Index Sensing ◽  
Fabry Perot ◽  
Pattern Characteristic ◽  
Near Infrared Range

Porous materials have become one of the best options for the development of optical sensors, since they maximize the interaction between the optical field and the target substances, which boosts the sensitivity. In this work, we propose the use of a readily available mesoporous material for the development of such sensors: commercial polycarbonate track-etched membranes. In order to demonstrate their utility for this purpose, we firstly characterized their optical response in the near-infrared range. This response is an interference fringe pattern, characteristic of a Fabry–Pérot interferometer, which is an optical device typically used for sensing purposes. Afterwards, several refractive index sensing experiments were performed by placing different concentrations of ethanol solution on the polycarbonate track-etched membranes. As a result, a sensitivity value of around 56 nm/RIU was obtained and the reusability of the substrate was demonstrated. These results pave the way for the development of optical porous sensors with such easily available mesoporous material.

Changes in Optical Properties of in vitro Myocardium Tissue Due to Heating in the Visible and Near-Infrared Range

2008 ◽  
Vol 35 (5) ◽  
pp. 792-796 ◽  
Author(s):  
敖荟兰 Ao Huilan ◽  
邢达 Xing Da ◽  
魏华江 Wei Huajiang ◽  
巫国勇 Wu Guoyong ◽  
鲁建军 Lu Jianjun
Keyword(s):  
Optical Properties ◽  
Near Infrared ◽  
Infrared Range ◽  
Near Infrared Range

scholarly journals Spektroskopi Reflektansi Sampel Tanah dan Batuan yang Mengandung Mineral Pembawa Unsur Tanah Jarang dan Radioaktif

EKSPLORIUM ◽  
2019 ◽  
Vol 40 (2) ◽  
pp. 89
Author(s):  
Arie Naftali Hawu Hede ◽  
Muhammad Anugrah Firdaus ◽  
Yogi La Ode Prianata ◽  
Mohamad Nur Heriawan ◽  
Syafrizal Syafrizal ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Rare Earth ◽  
Optical Sensors ◽  
Near Infrared ◽  
Spectral Characteristics ◽  
Infrared Range ◽  
Basic Principles ◽  
Spectral Signatures ◽  
Shortwave Infrared ◽  
Remote Sensing Methods

ABSTRAKSpektroskopi reflektansi merupakan salah satu metode nondestruktif untuk identifikasi mineral dan sebagai dasar dalam analisis pengindraan jauh (indraja) sensor optik. Penelitian ini bertujuan melakukan kajian penerapan spektroskopi reflektansi pada panjang gelombang 350–2.500 nm untuk sampel tanah dan batuan pembawa unsur tanah jarang (rare earth element-REE) dan radioaktif. Sampel diambil dari beberapa lokasi di Bangka Selatan dan Mamuju yang sebelumnya telah diidentifikasi memiliki potensi REE dan unsur radioaktif. Kurva reflektansi hasil analisis sampel dari Bangka Selatan menunjukan adanya kenampakan absorpsi yang menjadi karakteristik untuk kehadiran REE, dalam bentuk mineral monasit, zirkon, dan xenotime khususnya pada sampel yang berasal dari material tailing dan konsentrat bijih timah. Panjang gelombang yang menjadi kunci khususnya berada pada rentang visible-near infrared (VNIR; 400–1.300 nm). Sedangkan untuk sampel yang berasal dari Mamuju, yang merupakan daerah prospeksi mineral radioaktif, karakteristik spektral memperlihatkan beberapa panjang gelombang kunci terutama pada rentang shortwave infrared (1.300–2.500 nm). Hasil interpretasi menunjukkan mineral mayor berupa mineral lempung, sulfat, spesies NH4, dan mineral yang mengandung Al-OH lainnya, sedangkan untuk beberapa sampel pada panjang gelombang VNIR diidentifikasi mengandung mineral besi oksida/hidroksida. Hasil penelitian ini diharapkan dapat berguna untuk pemetaan eksplorasi REE dan radioaktif dengan menggunakan metode indraja.ABSTRACTReflectance spectroscopy is one of the nondestructive methods of mineral identification and is one of the basic principles in the remote sensing analysis using optical sensors. This research aimed at applying reflectance spectroscopy at 350–2,500 nm wavelength range for samples containing rare earth elements (REE) and radioactive minerals. Samples were taken from several locations in South Bangka and Mamuju that had previously been identified as potential location of REE and radioactive-bearing minerals. Reflectance data shows that there are absorption characteristics for REE-bearing minerals; monazite, zircon, and xenotime minerals especially from tailings and tin ore concentrate for the samples from South Bangka. The key wavelengths are specifically in the visible-near infrared range (VNIR; 400–1300 nm). For the samples from Mamuju, which is known as radioactive mineral prospecting areas, spectral characteristics provide information that there are spectral signatures in the shortwave infrared range (1,300–2,500 nm). The results of major mineral interpretations include clay minerals, sulfates, NH4 species, and other minerals containing Al-OH. However, some samples at the VNIR wavelength identified as iron oxide/hydroxide minerals. It is hoped that these results can be useful for REE and radioactive exploration mapping using remote sensing methods.

scholarly journals Hyperspectral system trade-offs for illumination, hardware and analysis methods: a case study of seed mix ingredient discrimination

2020 ◽  
Author(s):  
Carolina Blanch-Pérez del Notario ◽  
Carlos López-Molina ◽  
Andy Lambrechts ◽  
Wouter Saeys
Keyword(s):  
Hyperspectral Imaging ◽  
Spectral Resolution ◽  
Food Industry ◽  
Near Infrared ◽  
Imaging System ◽  
Infrared Range ◽  
Analysis Methods ◽  
Trade Offs ◽  
The Impact

The discrimination power of a hyperspectral imaging system for image segmentation or object detection is determined by the illumination, the camera spatial–spectral resolution, and both the pre-processing and analysis methods used for image processing. In this study, we methodically reviewed the alternatives for each of those factors for a case study from the food industry to provide guidance in the construction and configuration of hyperspectral imaging systems in the visible near infrared range for food quality inspection. We investigated both halogen- and LED-based illuminations and considered cameras with different spatial–spectral resolution trade-offs. At the level of the data analysis, we evaluated the impact of binning, median filtering and bilateral filtering as pre- or post-processing and compared pixel-based classifiers with convolutional neural networks for a challenging application in the food industry, namely ingredient identification in a flour–seed mix. Starting from a basic configuration and by modifying the combination of system aspects we were able to increase the mean accuracy by at least 25 %. In addition, different trade-offs in performance-complexity were identified for different combinations of system parameters, allowing adaptation to diverse application requirements.

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