scholarly journals Impact of remnant healthy pulp and apical tissue on outcomes after simulated regenerative endodontic procedure in rat molars

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Naoki Edanami ◽  
Kunihiko Yoshiba ◽  
Mari Shirakashi ◽  
Razi Saifullah Ibn Belal ◽  
Nagako Yoshiba ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Root Apex ◽  
Apical Periodontitis ◽  
Mineralized Tissue ◽  
Immature Rat ◽  
Immature Teeth ◽  
Root Sheath ◽  
Tissue Healing ◽  
The Impact ◽  
Rat Molars

AbstractWhen regenerative endodontic procedures (REPs) are performed on immature teeth diagnosed with pulp necrosis and apical periodontitis, various healing patterns occur. Furthermore, infected immature teeth with endodontic disorders often exhibit some remnant pulp and apical tissue. Therefore, this study investigated the impact of remnant healthy or fully functional pulp and apical tissue on healing patterns after REPs. Simulated REPs were performed on non-infected immature rat molars with different amounts of remnant pulp and apical tissue. Healing patterns in these teeth were assessed after 28 days. Teeth with 0.81–0.91 mm of remnant pulp healed with pulp-like tissue, dentin, and osteodentin-like dentin-associated mineralized tissue (OSD-DAMT); teeth with 0.60–0.63 mm of remnant pulp healed with pulp-like tissue and OSD-DAMT; teeth with 0.13–0.43 mm of remnant pulp healed with periodontal ligament (PDL)-like tissue, OSD-DAMT, and cementum-like dentin-associated mineralized tissue (CEM-DAMT); and teeth with disorganization of pulp and apical tissues at 0.15–0.38 mm beyond the root apex healed with PDL-like tissue, CEM-DAMT, and intracanal bone (IB). Loss of Hertwig’s epithelial root sheath was observed with IB formation. These results showed that four distinct healing patterns occurred after REPs, depending on the preoperative amount of remnant healthy pulp and apical tissue.

scholarly journals BiodentineTM Full Pulpotomy in Mature Permanent Teeth with Irreversible Pulpitis and Apical Periodontitis

Healthcare ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 720
Author(s):  
Xuan Vinh Tran ◽  
Lan Thi Quynh Ngo ◽  
Tchilalo Boukpessi
Keyword(s):  
Root Canal ◽  
Periodontal Ligament ◽  
Clinical Signs ◽  
Apical Periodontitis ◽  
Permanent Teeth ◽  
Definitive Treatment ◽  
Capping Agent ◽  
Root Canal Therapy ◽  
Pulp Capping ◽  
Irreversible Pulpitis

Vital pulp therapy, including direct pulp capping and partial and full pulpotomy, is primarily indicated for immature or mature permanent teeth with reversible pulpitis. Mature permanent teeth with irreversible pulpitis are frequently treated with root canal therapy. This report presents two cases of full pulpotomy using BiodentineTM in mature permanent teeth with irreversible pulpitis and acute apical periodontitis. The periapical radiograph illustrated a deep carious lesion extended to the pulp with apical radiolucency lesion or widened periodontal ligament space. Full pulpotomy with a tricalcium silicate-based cement was chosen as the definitive treatment. After decayed tissue excavation under a rubber dam, the exposed pulp tissue was amputated to the level of the canal orifice with a new sterile bur. BiodentineTM was applied as the pulp capping agent after hemostasis was obtained and for temporary restoration. The clinical signs disappeared quickly after the treatment. After one month, the coronal part of the temporary restoration was removed, and a composite resin was placed over the capping agent as a final restoration. At two-year follow-ups, the teeth were asymptomatic. Radiographs showed healing of the periapical lesion and periodontal ligament. BiodentineTM full pulpotomy of mature permanent teeth with irreversible pulpitis and apical periodontitis can be an alternative option to root canal therapy.

Interaction of silicon and cadmium in Brassica juncea and Brassica napus

Biologia ◽  
2012 ◽  
Vol 67 (3) ◽  
Author(s):  
Zuzana Vatehová ◽  
Karin Kollárová ◽  
Ivan Zelko ◽  
Danica Richterová-Kučerová ◽  
Marek Bujdoš ◽  
...  
Keyword(s):  
Lateral Roots ◽  
Root Apex ◽  
Brassica Species ◽  
Inhibitory Effects ◽  
Cd Uptake ◽  
Primary Roots ◽  
Suberin Lamellae ◽  
Root And Shoot Growth ◽  
The Impact ◽  
Si Addition

AbstractThe objective of this study was to determine the effect of silicon (Si) and cadmium (Cd) on root and shoot growth and Cd uptake in two hydroponically cultivated Brassica species (B. juncea (L.) Czern. cv. Vitasso and B. napus L. cv. Atlantic). Both species are potentially usable for phytoextraction. Inhibitory effects of Cd on root elongation were diminished by the impact of Si. Primary roots elongation in the presence of Cd + Si compared with Cd was stronger and the number of lateral roots was lower in B. juncea than in B. napus. Cd content per plant was higher in B. napus roots and shoots compared with B. juncea. Suberin lamellae were formed closer to the root apex in Cd + Si than in Cd treated plants and this effect was stronger in B. napus than in B. juncea. Accelerated maturation of endodermis was associated with reduced Cd uptake. Cd decreased the content of chlorophylls and carotenoids in both species, but Si addition positively influenced the content of photosynthetic pigments which was higher in B. napus than in B. juncea. Si enhanced more substantially translocation of Cd into the shoot of B. napus than of B. juncea. Based on our results B. napus seems to be more suitable for Cd phytoextraction than B. juncea because these plants produce more biomass and accumulate higher amount of Cd. The protective effect of Si on Cd treated Brassica plants could be attributed to more extensive development of suberin lamellae in endodermis.

scholarly journals Apexification with Calcium Hydroxide: 27 Months Follow Up of a Case

2013 ◽  
Vol 25 (1) ◽  
pp. 42-45
Author(s):  
AFMA Chowdhury ◽  
A Alam ◽  
UK Sarkar ◽  
AA Mahmud ◽  
U Habiba ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Treatment Approach ◽  
Multidisciplinary Treatment ◽  
Young Patients ◽  
Dental Trauma ◽  
Immature Teeth ◽  
Root Sheath ◽  
Medicine Today ◽  
And Function

Dental trauma is common in young patients with immature teeth. When necrosis of pulp follows in such a tooth, apexification of the root is the most recognized and accepted treatment of choice. Calcium hydroxide paste is the most popular material used to induce the apical hard tissue barrier which may be composed of different tissues. Hertwig’s epithelial root sheath and (or) its remnants, the cell rests of Malassez may contribute to the apical closure. In the presented case, careful multidisciplinary treatment approach helped to regain esthetic and function. After 27 months the tooth showed every sign of success. DOI: http://dx.doi.org/10.3329/medtoday.v25i1.16071 Medicine Today 2013 Vol.25(1): 42-45

scholarly journals Expression of α-smooth muscle actin in the periodontal ligament during post-emergent tooth eruption

2018 ◽  
Vol 46 (6) ◽  
pp. 2423-2435
Author(s):  
Domna Dorotheou ◽  
Marie-Luce Bochaton-Piallat ◽  
Catherine Giannopoulou ◽  
Stavros Kiliaridis
Keyword(s):  
Smooth Muscle ◽  
Periodontal Ligament ◽  
Smooth Muscle Actin ◽  
Root Apex ◽  
Tooth Eruption ◽  
Muscle Actin ◽  
Adult Rats ◽  
Time Points ◽  
Male Wistar Rats ◽  
The Right

Objective This study was performed to explore the expression of α-smooth muscle actin (α-SMA) in the periodontal ligament (PDL) of young and adult rats during post-emergent tooth eruption in opposed and unopposed teeth at two time points: 3 and 15 days after antagonist loss. Methods Four-week-old (n = 20) and 22-week-old (n = 20) male Wistar rats were used. The right maxillary molar crowns were cut down. PDL samples were isolated from the first mandibular molars at two time points: 3 and 15 days after cut-down of the right maxillary molars. Quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining were performed to detect differences in α-SMA expression in the PDL tissues of unopposed versus opposed molars. Results α-SMA was upregulated in the PDL of the unopposed molars in the 3-day group of young rats. The region around the root apex of the unopposed molars in this group exhibited strong immunostaining for α-SMA. The expression level and immunoreactivity of α-SMA did not differ in both time points in young controls and among all the adult groups. Conclusion α-SMA-positive myofibroblasts are implicated in post-emergent tooth eruption of unopposed molars of young animals.

scholarly journals A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model

2020 ◽  
Vol 8 (8) ◽  
pp. 1174
Author(s):  
Ok-Jin Park ◽  
Moon-Hee Jeong ◽  
Eun-Hye Lee ◽  
Mi-Ran Cho ◽  
Jaehong Hwang ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Bradyrhizobium Japonicum ◽  
Alveolar Bone ◽  
Bacterial Species ◽  
Root Apex ◽  
Apical Periodontitis ◽  
Microbial Infection ◽  
Pulp Chamber ◽  
Experimental Rat Model ◽  
Histopathologic Changes

Apical periodontitis caused by microbial infection in the dental pulp is characterized by inflammation, destruction of the pulpal and periradicular tissues, and alveolar bone resorption. We analyzed the chronological changes in microbiota using a pyrosequencing-based approach combined with radiologic and histopathologic changes in a rat apical periodontitis model. During the three-week observation, the pulp and periapical area showed a typical progress of apical periodontitis. A total of 27 phyla, 645 genera, and 1276 species were identified. The root apex had a lower bacterial species diversity than the pulp chamber. Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were dominant phyla in both the pulp chamber and root apex. Remarkably, bacterial communities showed a tendency to change in the root apex based on the disease progression. At the genus level, Escherichia, Streptococcus, Lactobacillus, Rodentibacter, and Bacteroidetes were dominant genera in the pulp chamber. The most abundant genera in the root apex were Bradyrhizobium, Halomonas, and Escherichia. The species Azospirillum oryzae increased in the pulp chamber, whereas the species Bradyrhizobium japonicum and Halomonas stevensii were highly observed in the root apex as the disease progressed. The experimental rat model of apical periodontitis demonstrated a relationship between the microbiota and the apical periodontitis progression.

Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells

2013 ◽  
Vol 46 (10) ◽  
pp. 962-970 ◽  
Author(s):  
W. Zhu ◽  
X. Zhu ◽  
G. T.-J. Huang ◽  
G. S. P. Cheung ◽  
W. L. Dissanayaka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Platelet Rich Plasma ◽  
Apical Periodontitis ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Immature Teeth ◽  
Dental Pulp Tissue ◽  
Pulp Cells

Pulp Revascularization of Immature Teeth With Apical Periodontitis: A Clinical Study

2009 ◽  
Vol 35 (5) ◽  
pp. 745-749 ◽  
Author(s):  
Rui Yu Ding ◽  
Gary Shun-pan Cheung ◽  
Jie Chen ◽  
Xing Zhe Yin ◽  
Qian Qian Wang ◽  
...  
Keyword(s):  
Clinical Study ◽  
Apical Periodontitis ◽  
Immature Teeth ◽  
Pulp Revascularization

Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells

2016 ◽  
Vol 35 (9) ◽  
pp. 983-990 ◽  
Author(s):  
Xin Ge ◽  
Ying-Feng Liu ◽  
Yong Wong ◽  
Li-Zheng Wu ◽  
Ling Tan ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Viability ◽  
Periodontal Ligament ◽  
Tobacco Consumption ◽  
Periodontal Ligament Cells ◽  
Nicotine Treatment ◽  
Pdl Cells ◽  
Induced Apoptosis ◽  
The Impact

Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones. CD4+ T cell-mediated immune response is involved in the progression of periodontitis. Tobacco consumption increases the risk of periodontal disease. However, the impact of nicotine on the interaction between human periodontal ligament (PDL) cells and CD4+ T cells remains unrevealed. Our study aims to investigate the effect of nicotine on PDL cells and the cocultured CD4+ T cells. The PDL cell cultures were established by explants from healthy individuals, exposed to nicotine or α-bungarotoxin (α-BTX), and incubated solely or in combination with CD4+ T cells. Afterwards, cell viability, secreted cytokines, and matrix metalloproteinases (MMPs) were evaluated. In monoculture of PDL cells, nicotine dramatically repressed cell viability and increased apoptosis. Meanwhile, α-BTX largely reversed the nicotine-induced apoptosis and increased viability of PDL cells. Compared with the monoculture, MMP-1, MMP-3, interleukin (IL)-1β, IL-6, IL-17, and IL-21 in supernatant of cocultures were markedly elevated after treatment with nicotine. Moreover, α-BTX significantly attenuated nicotine-triggered production of these components either in mono- or co-cultures. In addition, PDL cell-derived CXCL12 following nicotine treatment recruited CD4+ T cells. Above all, nicotine deteriorated periodontitis partially by promoting PDL cell–CD4+ T cell-mediated inflammatory response and matrix degradation.

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