scholarly journals Fetal bovine serum albumin inhibits antimicrobial peptide activity and binds drug only in complex with α1-antitrypsin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wen-Hung Tang ◽  
Chiu-Feng Wang ◽  
You-Di Liao
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Culture Media ◽  
Cell Culture Media ◽  
Antibiotic Resistant ◽  
Hydrophobic Residues ◽  
Fetal Bovine ◽  
Fetal Stage

AbstractSeveral antimicrobial peptides (AMPs) have been developed for the treatment of infections caused by antibiotic-resistant microbes, but their applications are primarily limited to topical infections because in circulation they are bound and inhibited by serum proteins. Here we have found that some AMPs, such as TP4 from fish tilapia, and drugs, such as antipyretic ibuprofen, were bound by bovine serum albumin only in complex with α1-antitrypsin which is linked by disulfide bond. They existed in dimeric complex (2 albumin -2 α1-antitrypsin) in the bovine serum only at fetal stage, but not after birth. The hydrophobic residues of TP4 were responsible for its binding to the complex. Since bovine serum is a major supplement in most cell culture media, therefore the existence and depletion of active albumin/α1-antitrypsin complex are very important for the assay and production of biomolecules.

Tribological Performance of Cell-Treated Nickel Matrix

2005 ◽  
Author(s):  
Bing Shi ◽  
Thomas Kuhn ◽  
Lawrence Duffy ◽  
Hong Liang
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Dry Friction ◽  
Culture Media ◽  
Tribological Performance ◽  
Nickel Matrix ◽  
Cell Culture Media ◽  
Pin On Disk

In this study, effects of cell culture on surface properties, and tribological performance were investigated. The wettability of Ni under dry and lubricated conditions, as well as cell-cultured specimens was evaluated. The tribological performance of these samples was compared using a pin-on-disk tribometer. Dry friction tests were conducted and compared with the bovine serum albumin (BSA) solution lubricated Ni and the cell culture media lubricated Ni. The lubrication behavior was discussed and new biofluid mechanisms were proposed.

A Precipitin-like Reaction of the Hemolymph of the Lobster Homarus americanus

1969 ◽  
Vol 26 (5) ◽  
pp. 1392-1397 ◽  
Author(s):  
James E. Stewart ◽  
Diane M. Foley
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Test Period ◽  
Fluorescent Material

The levels of fluorescent material in the hemolymph of lobsters injected with serum proteins from lobster hemolymph labelled with fluorescein remained relatively constant over a 6-day test period; the levels in lobsters injected with bovine serum albumin labelled with fluorescein declined rapidly. A precipitin-like reaction was observed when lobster hemolymph serum was titrated with bovine serum albumin in vitro.

scholarly journals Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
In Vitro Cytotoxicity ◽  
Binding Energies ◽  
Docking Simulations ◽  
Fetal Bovine

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

scholarly journals Development of Sheep Embryos in vitro in a Medium Supplemented with Different Batches of Serum Albumin

1988 ◽  
Vol 41 (3) ◽  
pp. 371 ◽  
Author(s):  
PA Batt ◽  
BG Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Basal Medium ◽  
Mammalian Embryo ◽  
Molecular Weights ◽  
Mean Molecular Weight

Variability in different lots of commercial serum albumin affects mammalian embryo development in culture. The composition of commercial preparations of ovine, bovine and defatted bovine serum albumin and a fraction of ovine serum containing proteins with a mean molecular weight of 65 kDa (fraction 3) was examined by polyacrylamide gel electrophoresis. All preparations were heavily contaminated with serum proteins other than albumin. Day-6 sheep morulae were cultured for 48 h in a basal bicarbonate-buffered salt solution supplemented with the commercial preparations of ovine, bovine or defatted bovine serum albumin. These three albumin preparations differed in their abilities to support the development of morulae into expanded blastocysts, but these differences disappeared when the basal medium was also supplemented with a component of ovine serum containing substances with molecular weights of less than 10 kDa. In the latter case, the three commercial albumin preparations and fraction 3 of ovine serum all supported full development in about 40-60% of morulae.

Influence of l-carnitine on lipid metabolism of buffalo cumulus-oocyte complexes matured in either fetal bovine serum or fatty acid-free bovine serum albumin

2020 ◽  
Vol 158 ◽  
pp. 382-390
Author(s):  
Diego Fernando Dubeibe Marin ◽  
Nathalia Nogueira da Costa ◽  
Priscilla di Paula Bessa Santana ◽  
Eduardo Baia de Souza ◽  
Sebastião Tavares Rolim filho ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Electrochemical detection of N-homocysteinylated BSA in the fetal bovine serum medium

RSC Advances ◽  
2015 ◽  
Vol 5 (7) ◽  
pp. 4774-4779 ◽  
Author(s):  
Ece Eksin ◽  
Arzum Erdem
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Electrochemical Detection ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Homocysteine Thiolactone ◽  
Passive Adsorption ◽  
Fetal Bovine ◽  
Fetal Bovine Serum Medium

The immobilization of bovine serum albumin (BSA), homocysteine-thiolactone (HTL) andN-homocysteinylated BSA (N-Hcy-BSA) onto the surface of each PGE was performed by passive adsorption and the electrochemical detection of these components was investigated individually.

scholarly journals The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Márcia Regina Ribeiro Alves ◽  
Abraham Damian Giraldo Zuñiga ◽  
Rita de Cássia Superbi Sousa ◽  
Carmelita Zacchi Scolforo
Keyword(s):  
Bovine Serum Albumin ◽  
Zeta Potential ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Second Order ◽  
Maximum Adsorption Capacity ◽  
Pseudo Second Order ◽  
Synthetic Hydroxyapatite

Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (qm) and yield (%) (whereqm=87.95 mg g−1and 74.2%; 68.26 mg g−1and 68.6%; and 36.18 mg g−1, 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.

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