Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia

Abstract16S rRNA sequencing of human fecal samples has been tremendously successful in identifying microbiome changes associated with both aging and disease. A number of studies have described microbial alterations corresponding to physical frailty and nursing home residence among aging individuals. A gut-muscle axis through which the microbiome influences skeletal muscle growth/function has been hypothesized. However, the microbiome has yet to be examined in sarcopenia. Here, we collected fecal samples of 60 healthy controls (CON) and 27 sarcopenic (Case)/possibly sarcopenic (preCase) individuals and analyzed the intestinal microbiota using 16S rRNA sequencing. We observed an overall reduction in microbial diversity in Case and preCase samples. The genera Lachnospira, Fusicantenibacter, Roseburia, Eubacterium, and Lachnoclostridium—known butyrate producers—were significantly less abundant in Case and preCase subjects while Lactobacillus was more abundant. Functional pathways underrepresented in Case subjects included numerous transporters and phenylalanine, tyrosine, and tryptophan biosynthesis suggesting that protein processing and nutrient transport may be impaired. In contrast, lipopolysaccharide biosynthesis was overrepresented in Case and PreCase subjects suggesting that sarcopenia is associated with a pro-inflammatory metagenome. These analyses demonstrate structural and functional alterations in the intestinal microbiota that may contribute to loss of skeletal muscle mass and function in sarcopenia.

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Evaluation of the nutrition and function of cow and goat milk based on intestinal microbiota by metagenomic analysis

Food & Function ◽

10.1039/c7fo01780d ◽

2018 ◽

Vol 9 (4) ◽

pp. 2320-2327 ◽

Cited By ~ 10

Author(s):

Zhaoxia Wang ◽

Shuaiming Jiang ◽

Chenchen Ma ◽

Dongxue Huo ◽

Qiannan Peng ◽

...

Keyword(s):

16S Rrna ◽

Gut Microbiota ◽

Intestinal Microbiota ◽

High Throughput ◽

Goat Milk ◽

16S Rrna Sequencing ◽

Metagenomic Analysis ◽

Sequencing Technology ◽

Rrna Sequencing ◽

And Function

A high-throughput 16S rRNA sequencing technology was applied to study changes of the intestinal microbiota in mice after the administration of cow and goat milk. We show a correlation between the gut microbiota and the nutrients in milk.

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Differences in the intestinal microbiota between insecticide‐resistant and ‐sensitive Aedes albopictus based on full‐length 16S rRNA sequencing

MicrobiologyOpen ◽

10.1002/mbo3.1177 ◽

2021 ◽

Vol 10 (2) ◽

Author(s):

Haiyang Wang ◽

Chongxing Zhang ◽

Peng Cheng ◽

Yang Wang ◽

Hongmei Liu ◽

...

Keyword(s):

16S Rrna ◽

Intestinal Microbiota ◽

Aedes Albopictus ◽

Full Length ◽

16S Rrna Sequencing ◽

Rrna Sequencing

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WITHDRAWN: Evaluating the association between body weight and the intestinal microbiota of weaned piglets via 16S rRNA sequencing

Veterinary Microbiology ◽

10.1016/j.vetmic.2016.10.020 ◽

2016 ◽

Vol 196 ◽

pp. 55 ◽

Cited By ~ 13

Author(s):

Geon Goo Han ◽

Jun-Yeong Lee ◽

Gwi-Deuk Jin ◽

Jongbin Park ◽

Yo Han Choi ◽

...

Keyword(s):

Body Weight ◽

16S Rrna ◽

Intestinal Microbiota ◽

16S Rrna Sequencing ◽

Weaned Piglets ◽

Rrna Sequencing

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Impact of substrate material and chlorine/chloramine on the composition and function of a young biofilm microbial community as revealed by high-throughput 16S rRNA sequencing

Chemosphere ◽

10.1016/j.chemosphere.2019.125310 ◽

2020 ◽

Vol 242 ◽

pp. 125310 ◽

Cited By ~ 3

Author(s):

Weiying Li ◽

Qiaowen Tan ◽

Wei Zhou ◽

Jiping Chen ◽

Yue Li ◽

...

Keyword(s):

Microbial Community ◽

16S Rrna ◽

High Throughput ◽

Substrate Material ◽

16S Rrna Sequencing ◽

Rrna Sequencing ◽

And Function

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16S rRNA sequencing analysis of the correlation between the intestinal microbiota and body-mass of grass carp (Ctenopharyngodon idella)

Comparative Biochemistry and Physiology Part D Genomics and Proteomics ◽

10.1016/j.cbd.2020.100699 ◽

2020 ◽

Vol 35 ◽

pp. 100699

Author(s):

Shen-Tong Wang ◽

Xin-Zhan Meng ◽

Jia-Hua Zhang ◽

Ya-Fan Dai ◽

YuBang Shen ◽

...

Keyword(s):

16S Rrna ◽

Body Mass ◽

Intestinal Microbiota ◽

Grass Carp ◽

16S Rrna Sequencing ◽

Ctenopharyngodon Idella ◽

Sequencing Analysis ◽

Rrna Sequencing ◽

Grass Carp Ctenopharyngodon Idella ◽

16S Rrna Sequencing Analysis

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Evaluating the association between body weight and the intestinal microbiota of weaned piglets via 16S rRNA sequencing

Applied Microbiology and Biotechnology ◽

10.1007/s00253-017-8304-7 ◽

2017 ◽

Vol 101 (14) ◽

pp. 5903-5911 ◽

Cited By ~ 22

Author(s):

Geon Goo Han ◽

Jun-Yeong Lee ◽

Gwi-Deuk Jin ◽

Jongbin Park ◽

Yo Han Choi ◽

...

Keyword(s):

Body Weight ◽

16S Rrna ◽

Intestinal Microbiota ◽

16S Rrna Sequencing ◽

Weaned Piglets ◽

Rrna Sequencing

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Alleviation of Anxiety/Depressive-Like Behaviors and Improvement of Cognitive Functions by Lactobacillus plantarum WLPL04 in Chronically Stressed Mice

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2021/6613903 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Xuan Sun ◽

Hong-Fei Zhang ◽

Chao-Lin Ma ◽

Hua Wei ◽

Bao-Ming Li ◽

...

Keyword(s):

16S Rrna ◽

Lactobacillus Plantarum ◽

Chronic Stress ◽

Intestinal Microbiota ◽

Cognitive Functions ◽

16S Rrna Sequencing ◽

Enzyme Linked Immunosorbent Assay ◽

Brain Functions ◽

Rrna Sequencing ◽

Anxiety Depression

Background. Intestinal microorganisms play an important role in regulating the neurodevelopment and the brain functions of the host through the gut-brain axis. Lactobacillus, one of the most representative intestinal probiotics, produces important effects on human physiological functions. Our previous studies reveal that the Lactobacillus plantarum WLPL04 has a series of beneficial actions, such as antiadhesion of pathogens, protection from the harmful effect of sodium dodecyl sulfate, and anti-inflammatory stress on Caco2 cells. However, its effects on brain functions remain unknown. The present study aims to evaluate the potential effect of L. plantarum WLPL04 on anxiety/depressive-like behaviors in chronically restrained mice. Methods. Newly weaned mice were exposed to chronic restraint stress for four weeks and raised daily with or without L. plantarum WLPL04 water supplement. Animals were behaviorally assessed for anxiety/depression and cognitive functions. The 16S rRNA sequencing was performed to analyze the intestinal microbiota structure. The levels of the medial prefrontal cortical (mPFC) brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) and serum 5-hydroxytryptamine (5-HT) were examined using Western blot and enzyme-linked immunosorbent assay. Results. The chronic stress-induced anxiety/depressive-like behaviors and cognitive deficits were significantly alleviated by the L. plantarum WLPL04 treatment. The 16S rRNA sequencing analysis showed that the chronic stress reduced the diversity and the richness of intestinal microbiota, which were rescued by the L. plantarum WLPL04 treatment. The levels of BDNF and TrkB in the mPFC and the concentration of 5-HT in the serum remained unchanged in chronically restrained mice treated with the L. plantarum WLPL04. Conclusions. The L. plantarum WLPL04 can rescue anxiety/depressive-like behaviors and cognitive dysfunctions, reverse the abnormal change in intestinal microbiota, and alleviate the reduced levels of 5-HT, BDNF, and TrkB induced by chronic stress in mice, providing an experimental basis for the therapeutic application of L. plantarum on anxiety/depression.

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