Comparative genomics reveals insights into genetic variability and molecular evolution among sugarcane yellow leaf virus populations

AbstractYellow leaf disease caused by sugarcane yellow leaf virus (SCYLV) is one of the most prevalent diseases worldwide. In this study, six near-complete genome sequences of SCYLV were determined to be 5775–5881 bp in length. Phylogenetic analysis revealed that the two SCYLV isolates from Réunion Island, France, and four from China were clustered into REU and CUB genotypes, respectively, based on 50 genomic sequences (this study = 6, GenBank = 44). Meanwhile, all 50 isolates were clustered into three phylogroups (G1–G3). Twelve significant recombinant events occurred in intra- and inter-phylogroups between geographical origins and host crops. Most recombinant hotspots were distributed in coat protein read-through protein (RTD), followed by ORF0 (P0) and ORF1 (P1). High genetic divergences of 12.4% for genomic sequences and 6.0–24.9% for individual genes were determined at nucleotide levels. The highest nucleotide diversity (π) was found in P0, followed by P1 and RdRP. In addition, purifying selection was a main factor restricting variability in SCYLV populations. Infrequent gene flow between Africa and the two subpopulations (Asia and America) were found, whereas frequent gene flow between Asia and America subpopulations was observed. Taken together, our findings facilitate understanding of genetic diversity and evolutionary dynamics of SCYLV.

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Sugarcane Yellow Leaf Disease

EDIS ◽

10.32473/edis-sc074-2005 ◽

2019 ◽

Vol 2005 (12) ◽

Author(s):

Jack C. Comstock ◽

Robert A. Gilbert

Keyword(s):

Cooperative Extension Service ◽

Publication Date ◽

Yellow Leaf ◽

Yield Losses ◽

Electronic Publication ◽

Sugarcane Yellow Leaf Virus ◽

University Of Florida ◽

Leaf Disease ◽

Yellow Leaf Disease ◽

Leaf Virus

Sugarcane Yellow Leaf disease was first recognized as Yellow Leaf Syndrome during the 1980s in Hawaii. Later it was reportedly associated with yield losses of 25% or more in the cultivar SP 71-6163 in Brazil. Subsequently the virus sugarcane Yellow Leaf Virus (SCYLV) was discovered to be associated with the disease. Yellow leaf has been found in numerous countries of the world. However, somewhat confusedly, another disease, “leaf yellows”, has been found in other areas. Although it has similar symptoms, it is caused by a phytoplasm rather than a virus. To date, this phytoplasm pathogen has not been identified on sugarcane in Florida. This document is SS-AGR-256, one of a series of the Agronomy Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. This publication is also a part of the Florida Sugarcane Handbook, an electronic publication of the Agronomy Department. Original publication date: September 2005.

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Symptom expression of yellow leaf disease in sugarcane cultivars with different degrees of infection by Sugarcane yellow leaf virus

Plant Pathology ◽

10.1111/j.1365-3059.2007.01696.x ◽

2007 ◽

Vol 0 (0) ◽

pp. 070918211612010-??? ◽

Cited By ~ 1

Author(s):

A. T. Lehrer ◽

E. Komor

Keyword(s):

Symptom Expression ◽

Yellow Leaf ◽

Sugarcane Yellow Leaf Virus ◽

Leaf Disease ◽

Yellow Leaf Disease ◽

Leaf Virus

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Survey of Sugarcane Yellow Leaf Virus in the Canal Point Breeding and Cultivar Development Program

Agronomy ◽

10.3390/agronomy11101948 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1948

Author(s):

Sushma Sood ◽

Wayne R. Davidson ◽

Miguel Baltazar

Keyword(s):

Polyclonal Antibodies ◽

Development Program ◽

Yellow Leaf ◽

Yield Losses ◽

Sugarcane Yellow Leaf Virus ◽

Cultivar Development ◽

Yellow Leaf Disease ◽

Tissue Blot Immunoassay ◽

Seed Increase ◽

Leaf Virus

Sugarcane yellow leaf virus (SCYLV), a Polerovirus in the family Luteoviridea, causes yellow leaf disease (YLD). Yield losses from YLD have been reported from several countries in both symptomatic and asymptomatic sugarcane cultivars. The breeding nursery at Canal Point (CP) in 2016 and primary and secondary seed increases in the CP cultivar development program at grower’s farm from 2015 to 2019 were surveyed for SCYLV infection by the tissue-blot immunoassay using polyclonal antibodies raised against SCYLV. More than 32% of varieties in the CP breeding nursery were infected with SCYLV in 2016. The SCYLV data of primary and secondary seedcane increases from 2015 to 2019 showed that out of 54 varieties screened at different locations, 12 had no SCYLV-positive plants, 24 had less than 5%, 5 had 6% to 12%, and 13 had 20% to 75% of the plants infected with SCYLV. The SCYLV screenings in varieties in the primary and secondary seed increase plantings provide growers an opportunity to acquire virus-free clean seedcane by apical meristem propagation to minimize the spread of the SCYLV and avoid yield losses.

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