Survey of Sugarcane Yellow Leaf Virus in the Canal Point Breeding and Cultivar Development Program

Sugarcane yellow leaf virus (SCYLV), a Polerovirus in the family Luteoviridea, causes yellow leaf disease (YLD). Yield losses from YLD have been reported from several countries in both symptomatic and asymptomatic sugarcane cultivars. The breeding nursery at Canal Point (CP) in 2016 and primary and secondary seed increases in the CP cultivar development program at grower’s farm from 2015 to 2019 were surveyed for SCYLV infection by the tissue-blot immunoassay using polyclonal antibodies raised against SCYLV. More than 32% of varieties in the CP breeding nursery were infected with SCYLV in 2016. The SCYLV data of primary and secondary seedcane increases from 2015 to 2019 showed that out of 54 varieties screened at different locations, 12 had no SCYLV-positive plants, 24 had less than 5%, 5 had 6% to 12%, and 13 had 20% to 75% of the plants infected with SCYLV. The SCYLV screenings in varieties in the primary and secondary seed increase plantings provide growers an opportunity to acquire virus-free clean seedcane by apical meristem propagation to minimize the spread of the SCYLV and avoid yield losses.

Response of Peanut Varieties to Phosphorus and Rhizobium Inoculant Rates on Haplic Lixisols of Guinea Savanna Zone of Ghana

Peanut forms a major component of the predominantly cereal-based farming systems in Northern Ghana. However, yields are low, prompting the need to evaluate the effects of phosphorus (PR) and rhizobium inoculant (IR) rates on growth, nodulation, and yield of peanut varieties. On-station and on-farm experiments were conducted to determine the interaction effects of three P rates (0, 30, and 60 kg P2O5/ha), three IR rates (0, 3, and 6 g/kg seed), and two peanut varieties [Chinese and Nkatie Savannah Agricultural Research Institute (SARI)] on growth, nodulation, and yield of peanut on Haplic Lixisols of Northern Ghana. Both experiments were conducted using a split-split plot design replicated three times for the on-station experiment and on six farmer's fields (on-farm experiment). In both experiments, combined application of 60 kg P2O5/ha and IR at 6 g/kg seed increased pod number in the Nkatie SARI and Chinese varieties compared to their control counterparts. PR × V interaction influenced growth, effective nodule number, and podding capacity with 60 kg P2O5/ha combined with Nkatie SARI to produce significantly higher values. The interaction of IR × V improved pod number, nodule number, and harvest index, such that inoculant at 6 g/kg seed combined with Nkatie SARI gave the best performance. PR × IR also had a significant interactive influence on peanut grain yield. Higher grain yields were recorded from 60 kg P2O5/ha in combination with 6 g/kg seed of rhizobium inoculant. Therefore, these results suggest that the use of P fertilizer at 60 kg/ha and rhizobium inoculant at 6 g/kg seed increase peanut productivity on Haplic Lixisols in Northern Ghana. However, it was prudent not to suggest any recommendations from the P rates in interaction with IR, since the result between the on-station and on-farm experiments appeared not consistent.

Aerial High-Throughput Phenotyping Enabling Indirect Selection for Grain Yield at the Early-generation Seed-limited Stages in Breeding Programs

ABSTRACTBreeding programs for wheat and many other crops require one or more generations of seed increase before replicated yield trials can be sown. Extensive phenotyping at this stage of the breeding cycle is challenging due to the small plot size and large number of lines under evaluation. Therefore, breeders typically rely on visual selection of small, unreplicated seed increase plots for the promotion of breeding lines to replicated yield trials. With the development of aerial high-throughput phenotyping technologies, breeders now have the ability to rapidly phenotype thousands of breeding lines for traits that may be useful for indirect selection of grain yield. We evaluated early generation material in the irrigated bread wheat (Triticum aestivum L.) breeding program at the International Maize and Wheat Improvement Center to determine if aerial measurements of vegetation indices assessed on small, unreplicated plots were predictive of grain yield. To test this approach, two sets of 1,008 breeding lines were sown both as replicated yield trials and as small, unreplicated plots during two breeding cycles. Vegetation indices collected with an unmanned aerial vehicle in the small plots were observed to be heritable and moderately correlated with grain yield assessed in replicated yield trials. Furthermore, vegetation indices were more predictive of grain yield than univariate genomic selection, while multi-trait genomic selection approaches that combined genomic information with the aerial phenotypes were found to have the highest predictive abilities overall. A related experiment showed that selection approaches for grain yield based on vegetation indices could be more effective than visual selection; however, selection on the vegetation indices alone would have also driven a directional response in phenology due to confounding between those traits. A restricted selection index was proposed for improving grain yield without affecting the distribution of phenology in the breeding population. The results of these experiments provide a promising outlook for the use of aerial high-throughput phenotyping traits to improve selection at the early-generation seed-limited stage of wheat breeding programs.

Conditional dormancy of Stipa lagascae (Poaceae) bulk-harvested on seed increase plots in South Tunisia: a reassessment and a surprise

Background and aims – With the perspective to reseed degraded drylands, grass seeds are often stocked for several years. This common practice overlooks conditional dormancy and the necessity to preserve it. This paper reports on the germination ecology of Stipa lagascae Roem. & Schult., which is a circum-Mediterranean winter-growing bunch grass of high grazing value. However, the published record on its germination ecology is scarce and inconsistent.Methods – This record was reassessed through a series of germination trials in combination with dormancy breaking treatments on seeds that were mainly harvested on a seed increase plot in South-Tunisia.Key results –The surprise finding was that Stipa lagascae exhibits a particular kind of conditional dormancy for many months after harvest. Whereas dormant seeds barely germinate at 10°C in classical Petri dishes or on germination tables, they germinate massively (but not fully) when allowed full contact with a water-saturated substrate at 7–10°C in boxes. Dehulling provokes fast germination of near 100% of the seeds, thus showing that the substrate effect at low temperatures breaks most but not all dormancy in a particular seed lot. This remaining or residual dormancy is not conditional, as it can only be broken through dehulling. There are thus two distinct germination windows: a very broad one for non-dormant seed and a narrow one for conditionally dormant seed.Conclusions – A pattern is suggested whereby each seed lot evolves through a continuum from full over conditional to non-dormancy and finally mortality. However, only the state of conditional dormancy times germination optimally with regard to the start of the winter growing season in South-Tunisia. Its ecological significance should be interpreted in combination with its trypanocarpy. Reseeding for restoration purposes and to render grazing value to depleted drylands should thus use conditionally dormant seed.

Factors Affecting Adoption of Improved Crops by Rural Farmers in Niger

Improved crops are advocated to meet the dual challenge of food security and the fight against poverty in developing countries. As most poor people in developing countries live in rural areas and depend on agriculture for their livelihood, an important key to get them out of poverty is to increase agricultural productivity by using technologies such as improved crops. However, the rate of improved crops adoption remains surprisingly low in Niger, one of the world poorest countries. In this paper, we examine the factors affecting adoption of improved crops by rural farmers focusing on Niger. Using the 2014’s National Survey on Households Living Conditions and Agriculture, we investigate the effect of farmers’ socioeconomic characteristics, the farm’s quality, the geographic location, the production system, the access to improved seeds and the land tenure on the probability to use improved crops rather than local crops. Our results suggest that the ownership of a government land title is the most important driver in the adoption of improved crops by rural farmers. In addition, being a female, educated, practicing polyculture, having access to improved seed increase the probability to adopt improved crops. In contrast, household size, operating on the parcel for a long period and the parcel size reduces the probability to use improved crops. These determinants of improved crops adoption should be considered in Niger’s agricultural policy to succeed in the dissemination of improved crops among rural farmers.

Development and Application of Speed Breeding Technologies in a Commercial Peanut Breeding Program

ABSTRACT Reducing the release time of new peanut varieties is a key objective of peanut breeding programs. In the Australian Peanut Genetic Improvement Program release time has traditionally taken 10 to 15 years; however, the relatively recent use of winter breeding and seed increase nurseries has significantly reduced release times. Despite these improvements, full-season maturity cultivars are still limited to two generations in a calendar year, when grown under optimal environmental conditions. This paper describes a new speed breeding technique, which combines controlled environment conditions, continuous light in conjunction with optimal temperature, and a single seed descent breeding strategy in a greenhouse environment. Speed breeding was successful in reducing generation time of full-season maturity cultivars from 145 to 89 days. Speed breeding can rapidly progress the inbreeding of F2, F3 and F4 generations in less than 12 months, and potentially accelerate the development of first cross to commercial release in around six to seven years.