scholarly journals Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-Ju Kim ◽  
Young-Jin Kim ◽  
Wansu Park
Keyword(s):  
Calcium Release ◽  
Poly I:C ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Gm Csf ◽  
Polycytidylic Acid ◽  
Mouse Macrophages ◽  
Anti Inflammatory ◽  
Polyinosinic Polycytidylic Acid ◽  
Stat Pathway

AbstractBerberine is a well-known quaternary ammonium salt that is usually found in the roots of such plants as Phellodendron amurense and Coptis chinensis. However, the effects of berberine on double-stranded RNA (dsRNA)-induced macrophages have not been fully reported. In this study, we examined the anti-inflammatory effects of berberine on dsRNA [polyinosinic-polycytidylic acid; poly I:C]-induced macrophages. Levels of nitric oxide (NO), Prostaglandin E2 (PGE2), first apoptosis signal receptor (Fas; CD95), cytokines, intracellular calcium, phosphorylated I-kappa-B-alpha (IkB-α), phosphorylated p38 mitogen-activated protein kinase (MAPK), phosphorylated ERK1/2, phosphorylated signal transducer and activated transcription 3 (STAT3), and mRNA expression of inflammatory genes in poly I:C-induced RAW 264.7 mouse macrophages were evaluated. Berberine significantly inhibited the production of NO, PGE2, Fas, GM-CSF, LIF, LIX, RANTES, and MIP-2 as well as calcium release in poly I:C-induced RAW 264.7 cells at concentrations of up to 50 μM. Berberine also significantly inhibited the phosphorylation of p38 MAPK, ERK1/2, IkB-α, and STAT3 in poly I:C-induced RAW 264.7 cells. Additionally, berberine significantly decreased the mRNA expressions of Chop (GADD153), Stat1, Stat3, and Fas in poly I:C-induced RAW 264.7 cells. Taken together, berberine has anti-inflammatory properties related to its inhibition of NO, PGE2, Fas, GM-CSF, LIF, LIX, RANTES, and MIP-2 in dsRNA-induced macrophages via the endoplasmic reticulum stress-related calcium-CHOP/STAT pathway.

scholarly journals Anti-Inflammatory Effects of Diospyrin on Lipopolysaccharide-Induced Inflammation Using RAW 264.7 Mouse Macrophages

Biomedicines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 11 ◽  
Author(s):  
Adnan Shahidullah ◽  
Ji-Young Lee ◽  
Young-Jin Kim ◽  
Syed Muhammad Ashhad Halimi ◽  
Abdur Rauf ◽  
...  
Keyword(s):  
Leishmania Donovani ◽  
Calcium Release ◽  
Tumor Necrosis Factor Receptor ◽  
Mitogen Activated Protein Kinase ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Colony Stimulating Factor ◽  
Mouse Macrophages ◽  
Anti Inflammatory ◽  
Stimulating Factor

Diospyrin is a bisnaphthoquinonoid medicinal compound derived from Diospyros lotus, with known anti-cancer, anti-tubercular, and anti-leishmanial activities against Leishmania donovani. However, the effects of diospyrin on lipopolysaccharide (LPS)-induced macrophage activation and inflammation are not fully reported. In this study, the anti-inflammatory effects of diospyrin on LPS-induced macrophages were examined. Diospyrin showed no toxicity in RAW 264.7 at concentrations of up to 10 μM. Diospyrin moderated the production of nitric oxide (NO), monocyte chemotactic protein-1, macrophage inflammatory protein-1β, interleukin (IL)-6, IL-10, granulocyte colony-stimulating factor, granulocyte macrophage colony-stimulating factor, vascular endothelial growth factor, leukemia inhibitory factor, and RANTES/CCL5, as well as calcium release in LPS-induced RAW 264.7, at concentrations of up to 10 μM significantly (p < 0.05). Diospyrin also significantly inhibited the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and mRNA expression of C/EBP homologous protein (CHOP), as well as tumor necrosis factor receptor superfamily member 6 (Fas), in LPS-induced RAW 264.7 cells at concentrations of up to 10 μM (p < 0.05). Diospyrin exhibits anti-inflammatory properties mediated via inhibition of NO, and cytokines in LPS-induced mouse macrophages via the ER-stressed calcium-p38 MAPK/CHOP/Fas pathway.

scholarly journals Anti-Inflammatory Effect of Baicalein on Polyinosinic–Polycytidylic Acid-Induced RAW 264.7 Mouse Macrophages

Viruses ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 224 ◽  
Author(s):  
Young-Jin Kim ◽  
Hyun-Ju Kim ◽  
Ji Lee ◽  
Do-Hoon Kim ◽  
Mi Kang ◽  
...  
Keyword(s):  
Raw 264.7 ◽  
Polycytidylic Acid ◽  
Mouse Macrophages ◽  
Anti Inflammatory ◽  
Polyinosinic Polycytidylic Acid ◽  
Inflammatory Effect

scholarly journals Anti-Inflammatory Effect of 3-Methylcarbazoles on RAW 264.7 Cells Stimulated with LPS, Polyinosinic-Polycytidylic Acid and Pam3CSK

2012 ◽  
Vol 02 (02) ◽  
pp. 98-103 ◽  
Author(s):  
Thongchai Taechowisan ◽  
Srisakul Chanaphat ◽  
Wanwikar Ruensamran ◽  
Waya S. Phutdhawong
Keyword(s):  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Polycytidylic Acid ◽  
Anti Inflammatory ◽  
Polyinosinic Polycytidylic Acid ◽  
Inflammatory Effect

scholarly journals A Combination Extract of Gardeniae Fructus and Perillae Folium Exerts Anti-Inflammatory Effects on LPS-Activated RAW 264.7 Mouse Macrophages via an ER Stress-Induced CHOP Pathway

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1632
Author(s):  
Wansu Park
Keyword(s):  
Er Stress ◽  
P38 Mapk ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Mrna Levels ◽  
Mapk Phosphorylation ◽  
Inflammatory Reactions ◽  
Inflammatory Protein ◽  
Mouse Macrophages ◽  
Anti Inflammatory

The aim of this study is to investigate the effects of a combination extract of Gardeniae Fructus and Perillae Folium (GP) on inflammatory reactions in lipopolysaccharide (LPS)-activated mouse macrophages RAW 264.7 cells. Multiplex cytokine assay, Fluo-4 calcium assay, Flow cytometry assay for phospho-P38 MAPK, and quantitative PCR were carried out. GP significantly reduced LPS-induced productions of macrophage inflammatory protein (MIP)-1α and monokine induced by gamma interferon (MIG) and release of intracellular calcium in LPS-activated RAW 264.7 cells. GP also significantly inhibited P38 MAPK phosphorylation and mRNA levels of Chop, Camk2a, Stat1, Stat3, Jak2, Fas, Nos2, and Ptgs2 in LPS-activated RAW 264.7 cells. Taken together, this study represents that GP exerts anti-inflammatory effects on LPS-activated RAW 264.7 cells via ER stress-induced CHOP pathway.

scholarly journals Demonstrating the relationship between the phytochemical profile of different teas with relative antioxidant and anti-inflammatory capacities

2017 ◽  
Vol 7 (6) ◽  
pp. 375 ◽  
Author(s):  
Xiu-Min Chen ◽  
David D Kitts ◽  
Zhili Ma
Keyword(s):  
Camellia Sinensis ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Gm Csf ◽  
Rooibos Tea ◽  
Ifn Γ ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Stimulating Factor

Background: Indigenous or traditional aqueous plant extracts are commonly used by as much as 80% of the world’s population for primary health needs. Teas including Camellia sinensis teas and herbal teas were characterized for phytochemical content and the potential to offer specific bioactivities that could benefit human health by mitigating oxidative stress and inflammation. Context and purpose of this study: In present study, we compared the phytochemical profiles, antioxidant and anti-inflammatory activities of four Camellia sinensis (white, green, oolong, and black) teas and two herbal (Rooibos and Yerba mate) teas that are produce and consumed by different population worldwide. We also studied the impact of Rooibos tea on the production of inflammatory mediators, including nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) and different cytokines in Raw 264.7 cells, both with or without interferon γ (IFN-γ) and lipopolysaccharide (LPS) stimulation.Results: White tea had the highest total phenolic content (TPC) and antioxidant activity among these six teas examined, whereas, Rooibos tea has the lowest TPC, antioxidant and anti-inflammatory activities. Yerba mate tea had the greatest potential to inhibit NO production in IFN-γ and LPS-induced Raw 264.7 cells. The anti-inflammatory activity of teas was found to be correlated with antioxidant activity and phytochemical composition. Among the six teas examined, only Rooibos tea was found to induce NO in unstimulated Raw 264.7 cells. Under basal conditions, Rooibos tea induced interleukin (IL)-1α (IL-1α), IL-1β, IL-6, IL-10, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor- alpha (TNF-α), iNOS and COX-2 production. However, Rooibos tea also showed a dose-dependent inhibition of IL-6, IL-10, iNOS and COX-2 expression in stimulated Raw 264.7 cells. Although high concentration Rooibos tea was affective to inhibit induced IL-1α, G-CSF and GM-CSF, low concentration Rooibos tea also can up-regulate the expression of these cytokines. No inhibitory effects of all teas examined were found on mitigation of IL-1β and TNF-α.Conclusions: Rooibos tea can show dual functions on inflammation, by either promoting an inflammatory response to cytokines induction or, alternatively inhibit inflammation on exposure to cytokine treatment, such as tissue injury or a pathogen infection. Rooibos tea also has marked value in mitigating disease states such as hypertension and cardiovascular diseases where induction of NO production is important.Keywords: Camellia sinensis, Rooibos tea, herbal tea, inflammation, cytokine, antioxidant

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