scholarly journals MiR-124 and miR-506 are involved in the decline of protein C in children with extra-hepatic portal vein obstruction

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin-Shan Zhang ◽  
Long Li
Keyword(s):  
Blood Flow ◽  
Protein C ◽  
Portal Blood Flow ◽  
Negative Control ◽  
Protein Levels ◽  
Qrt Pcr ◽  
Candidate Mirnas ◽  
Rex Shunt ◽  
Cox 2 ◽  
Hepatic Portal

AbstractThe deficiency of protein C (PROC) can be partly rescued by Rex shunt through restoring portal blood flow in children with extra-hepatic portal venous obstruction (EHPVO). However, the decline of PROC is still found in some patients with a normal portal blood flow after Rex shunt. The aim of this study was to identify the candidate miRNAs involving in the decline of PROC and their mechanism. The protein level of PROC was detected by the ELISA assay, and was compared between sick and healthy groups. The expressions of miRNAs and PROC mRNA were measured using qRT-PCR, and were compared between sick and healthy groups. The correlation between PROC and candidate miRNAs was analysed by a Pearson correlation analysis to identify the most significant miRNAs. The expression of PROC mRNA was detected by qRT-PCR in HL-7702 and LX-2 cells tansfected with miRNAs mimics or inhibitors and negative control (NC) mimics, which was compared among the different groups. The rates of liver cells’ proliferation and apoptosis were detected in HL-7702 and LX-2 cells tansfected with miRNAs mimics or inhibitors or with overexpressing PROC and negative control mimics by CKK8 assay and flow cytometry, which were compared among the different groups. The expressions of COX-2 and VEGF were measured by qRT-PCR, and were compared between the miRNAs groups and NC group. Western blot was assayed for detecting the protein levels of PROC, COX-2, VEGF, Bcl-2 and Bax, which were compared between the miRNAs groups and NC group. The expression of PROC mRNA was lower, and the expressions of miR-506-3p and miR-124-3p were higher in children with EHPVO than healthy group. PROC mRNA was negatively correlated with the expression of miR-506-3p and miR-124-3p. Compared to the NC group, the transcription activity of PROC was lower after exposure of miR-506 and miR-124 mimics in HL-7702 and LX-2 cells, but this phenomenon was reversed after inhibiting miR-506 and miR-124. The rate of cell proliferation was lower after exposure of miR-506 and miR-124 than the NC group, which was increased after inhibiting miR-506 and miR-124 in HL-7702 cells and overexpressing PROC in LX-2 cells. The apoptotic rate was higher after exposure of miR-506 and miR-124 than the NC group, which was decreased after inhibiting miR-506 and miR-124 in HL-7702 cells and overexpressing PROC in LX-2 cells. The mRNA levels of COX-2 and VEGF were significantly higher after exposure of miR-506 and miR-124 mimics than those in the NC group. The protein levels of PROC and Bcl-2 were down-regulated, and the levels of COX-2, Bax and VEGF were up-regulated after exposure of miR-506 and miR-124 in HL-7702 cells, but this phenomenon was reversed after inhibiting miR-506 and miR-124. MiR-506-3p and miR-124-3p may involve in the decline of PROC in protein and transcriptional level, in which the anti-proliferation and pro-apoptosis role of miR-506-3p and miR-124-3p for liver cells may involve in this mechanism.

Enhanced hepatic portal blood flow induced by prostaglandin E1 following liver transplantation in pigs

Surgery Today ◽  
1994 ◽  
Vol 24 (7) ◽  
pp. 621-626 ◽  
Author(s):  
Kazuhiro Tsukada ◽  
Takeo Sakaguchi ◽  
Takemi Tomiyama ◽  
Katsuyuki Uchida ◽  
Yoshinobu Sato ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Blood Flow ◽  
Prostaglandin E1 ◽  
Portal Blood Flow ◽  
Portal Blood ◽  
Hepatic Portal

Quantification of in vitro and in vivo energy metabolism of the gastrointestinal tract of fed or fasted sheep

1993 ◽  
Vol 73 (4) ◽  
pp. 855-868 ◽  
Author(s):  
J. M. Kelly ◽  
B. G. Southorn ◽  
C. E. Kelly ◽  
L. P. Milligan ◽  
B. W. McBride
Keyword(s):  
Blood Flow ◽  
Gastrointestinal Tract ◽  
Portal Blood Flow ◽  
Whole Body ◽  
Hepatic Portal Vein ◽  
Flow Through ◽  
Hepatic Portal ◽  
Ouabain Inhibition

The effect of level of nutrition on in vitro and in vivo O2 consumption by the gastrointestinal tract in four nonlactating, nonpregnant ewes catheterized in the anterior mesenteric vein, hepatic portal vein and mesenteric artery with duodenal cannulae was investigated. Animals were fed a pelleted ration at maintenance (M) or twice maintenance (2M) or fasted (F) subsequent to the M measurement. Duodenal in vitro O2, ouabain-sensitive O2 (OSO2) and cycloheximide-sensitive O2 (CSO2) consumption was determined polarographically using a YSI O2 monitor; whole-gut O2 consumption was determined as (arterio-venous difference of O2 concentration) × (blood flow through the PV). Whole-body O2 consumption was determined using indirect calorimetry. Ewes fed 2M exhibited higher (P < 0.10) whole-body O2 consumption than either M or F ewes. Ewes fed M and 2M had higher (P < 0.10) duodenal in vitro O2 and ouabain-insensitive O2 (OIO2) consumption than F ewes. Hepatic portal blood flow was directly proportional to level of intake (P < 0.10): it was lowest for F ewes (81.0 L h−1), intermediate for M ewes (97.7 L h−1) and highest for 2M ewes (122.5 L h−1). Ouabain inhibition of O2 consumption by portal-drained viscera (PDV) was highest in M ewes and lowest in 2M ewes (P < 0.10). CSO2 consumption by the entire PDV was not affected by level of intake, corresponding to no change in OIO2 consumption by the PDV. As a proportion of whole-body O2 consumption, total O2, OSO2 and cycloheximide-insensitive O2 consumption by the PDV was higher in F ewes than in 2M ewes (P < 0.10). Fasted ewes expended a greater proportion of whole-body O2 consumption on gastrointestinal energetics than did 2M ewes. Key words: Sheep, gastrointestinal oxygen consumption, sodium–potassium ATPase, protein synthesis

scholarly journals Structural Design and Performance of the First Hepatic Portal Blood Flow Blocker

2020 ◽  
Author(s):  
GengQiang Shi ◽  
Shuai S. Yin
Keyword(s):  
Blood Flow ◽  
Portal Blood Flow ◽  
Blocking Effect ◽  
Quick Recovery ◽  
Experimental Apparatus ◽  
Blocking Device ◽  
And Performance ◽  
Set Up ◽  
Hepatic Portal

Abstract Laparoscopic surgery has been gradually promoted by people because of its advantages of small trauma and quick recovery. However, the operation is difficult, the first hepatic portal should be completely fastened during the operation. In this paper, through the study of the existing structure of the blocker, a kind of blood flow blocker for the first hepatic hilum blocking under laparoscope is designed. All kinds of parameters were calibrated through equation calculation, and the pressure guiding the blood flow blocking of hepatic portal during operation was calculated. The dynamic analysis was carried out with ANSYS software, and it was found that the fluid movement state was most uniform when the airflow velocity reached 8m/s. The experimental apparatus was set up to simulate the process of hepatic portal vein being blocked in vitro, then the feasibility of blocking effect was evaluated. Finally, it is concluded that the designed blood flow blocking device can have good blocking effect on blood vessels.

INFLUENCE OF IONOPHORE ADDITION TO ROUGHAGE AND HIGH-CONCENTRATE DIETS ON PORTAL BLOOD FLOW AND NET NUTRIENT FLUX IN CATTLE

1988 ◽  
Vol 68 (2) ◽  
pp. 419-429 ◽  
Author(s):  
D. L. HARMON ◽  
T. B. AVERY ◽  
G. B. HUNTINGTON ◽  
P. J. REYNOLDS
Keyword(s):  
Blood Flow ◽  
Oxygen Consumption ◽  
Latin Square ◽  
Portal Blood Flow ◽  
Nutrient Flux ◽  
Portal Blood ◽  
Beef Steers ◽  
Beef Heifers ◽  
Net Flux ◽  
Hepatic Portal

Two experiments using cattle with chronic hepatic-portal and arterial catheters were conducted to investigate effects of ionophore addition to roughage and high-concentrate diets on net nutrient flux across portal-drained viscera. Monensin and salinomycin added to a high-concentrate diet fed to three beef heifers in a Latin square design (exp. 1) increased the net portal flux of propionate (P < 0.05) and glutamate (P < 0.05) with a tendency for increased portal blood flow. Two beef steers (exp. 2) were fed alfalfa with monensin (300 mg d−1) for 22 d prior to dietary monensin and samples were taken on days −3, 0, 1,2, 4, 9, 16, 32, and 35 relative to monensin removal. Portal blood flow and gut oxygen consumption decreased (P < 0.05) following monensin removal until day 9, then remained relatively constant. Use of glucose by portal-drained viscera decreased (P < 0.05) until day 16, then returned towards initial values, while net appearance of L-lactate decreased linearly (P < 0.05). After monensin removal from the diet, the net portal flux of L-lactate, ammonia-N, acetate, propionate, isobutyrate, and 3-methylbutyrate were reduced (P < 0.05). These reductions were largely the result of decreased portal blood flow. In both experiments, dietary ionophores affected net flux across portal-drained viscera for several metabolites. These changes may differ, however, with roughage level and ionophore fed. Key words: Bovine, ionophore, absorption, blood flow

The effects of feeding and acute cold exposure on the visceral release of volatile fatty acids, estimated hepatic uptake of propionate and release of glucose, and plasma insulin concentration in sheep

1978 ◽  
Vol 39 (1) ◽  
pp. 219-226 ◽  
Author(s):  
G. E. Thompson ◽  
J. M. Bassett ◽  
A. W. Bell
Keyword(s):  
Blood Flow ◽  
Cold Exposure ◽  
Plasma Insulin ◽  
Volatile Fatty Acids ◽  
Environmental Temperature ◽  
Portal Blood Flow ◽  
Portal Blood ◽  
Insulin Concentration ◽  
Plasma Insulin Concentration ◽  
Hepatic Portal

1. Five sheep were given a meal while they were in a neutral environmental temperature (15–20°) and while acutely exposed to a moderately cold (1°, wind speed 2 m/s) environment.2. Before and at various times after feeding measurements were made of hepatic portal blood flow and the concentration of volatile fatty acids (VFA) and glucose in arterial, hepatic portal and hepatic venous blood plasma. From these measurements the net rate of release of VFA from the viscera was calculated, and the uptake of propionate and output of glucose by the liver was estimated, assuming hepatic arterial blood flow to be 20% of portal flow. The concentration of insulin in arterial and portal venous plasma was also measured.3. The change in environmental temperature did not affect the time taken by the animals to eat the meal completely.4. After feeding, in the neutral environment, there were significant increases in portal blood flow and release of VFA into the portal bloodstream. The uptake of propionate by the liver increased, significantly, and output of glucose also increased, but not significantly. Plasma insulin concentration also increased after feeding.5. During cold exposure portal blood flow was consistently higher, before and after feeding, than it was in the neutral environment. The release of VFA into the portal blood was also consistently greater during cold exposure, especially the release of propionate after feeding. Associated with this was an extra uptake of propionate and output of glucose by the liver. Plasma insulin concentration was slightly higher in the cold environment than the neutral environment before the animals were fed, but this difference was not apparent at any other time.

Portal Blood Flow as Measured by the Doppler Shift Technique in Sheep Fed Chopped Forages

1975 ◽  
Vol 41 (2) ◽  
pp. 596-600 ◽  
Author(s):  
L. R. Prewitt ◽  
D. R. Jacobson ◽  
R. W. Hemken ◽  
R. H. Hatton
Keyword(s):  
Blood Flow ◽  
Doppler Shift ◽  
Portal Blood Flow ◽  
Portal Blood ◽  
Shift Technique
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