scholarly journals Gene expression profiling of perineural invasion in head and neck cutaneous squamous cell carcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Timothy J. Eviston ◽  
Elahe Minaei ◽  
Simon A. Mueller ◽  
Navid Ahmadi ◽  
Bruce Ashford ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Expression Analysis ◽  
Squamous Cell ◽  
Perineural Invasion ◽  
Expression Profiles ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Mesenchymal Transition

AbstractPerineural invasion (PNI) is frequently associated with aggressive clinical behaviour in head and neck cutaneous squamous cell carcinoma (HNcSCC) leading to local recurrence and treatment failure. This study evaluates the gene expression profiles of HNcSCC with PNI using a differential expression analysis approach and constructs a tailored gene panel for sensitivity and specificity analysis. 45 cases of HNcSCC were stratified into three groups (Extensive, Focal and Non PNI) based on predefined clinicopathological criteria. Here we show HNcSCC with extensive PNI demonstrates significant up- and down-regulation of 144 genes associated with extracellular matrix interactions, epithelial to mesenchymal transition, cell adhesion, cellular motility, angiogenesis, and cellular differentiation. Gene expression of focal and non PNI cohorts were indistinguishable and were combined for further analyses. There is clinicopathological correlation between gene expression analysis findings and disease behaviour and a tailored panel of 10 genes was able to identify extensive PNI with 96% sensitivity and 95% specificity.

scholarly journals Gene Expression Profiling Of Perineural Invasion In Head And Neck Cutaneous Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Timothy J. Eviston ◽  
Elahe Minaei ◽  
Simon A. Mueller ◽  
Navid Ahmadi ◽  
Bruce Ashford ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Expression Analysis ◽  
Squamous Cell ◽  
Perineural Invasion ◽  
Expression Profiles ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Mesenchymal Transition

Abstract Perineural invasion (PNI) is frequently associated with aggressive clinical behaviour in head and neck cutaneous squamous cell carcinoma (HNcSCC) leading to local recurrence and treatment failure. This study evaluates the gene expression profiles of HNcSCC with PNI using a differential expression analysis approach and constructs a tailored gene panel for sensitivity and specificity analysis. 45 cases of HNcSCC were stratified into three groups (Extensive, Focal and Non PNI) based on predefined clinicopathological criteria. Here we show HNcSCC with extensive PNI demonstrates significant up- and down-regulation of 144 genes associated with extracellular matrix interactions, epithelial to mesenchymal transition, cell adhesion, cellular motility, angiogenesis, and cellular differentiation. Gene expression of focal and non PNI cohorts were indistinguishable and were combined for further analyses. There is clinicopathological correlation between gene expression analysis findings and disease behaviour and a tailored panel of 10 genes was able to identify extensive PNI with 96% sensitivity and 95% specificity.

scholarly journals Reprogramming and Differentiation of Cutaneous Squamous Cell Carcinoma Cells in Recessive Dystrophic Epidermolysis Bullosa

2020 ◽  
Vol 22 (1) ◽  
pp. 245
Author(s):  
Avina Rami ◽  
Łukasz Łaczmański ◽  
Jagoda Jacków-Nowicka ◽  
Joanna Jacków
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epidermolysis Bullosa ◽  
Expression Profiles ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Rapid Progression ◽  
Dystrophic Epidermolysis Bullosa ◽  
Recessive Dystrophic Epidermolysis Bullosa

The early onset and rapid progression of cutaneous squamous cell carcinoma (cSCC) leads to high mortality rates in individuals with recessive dystrophic epidermolysis bullosa (RDEB). Currently, the molecular mechanisms underlying cSCC development in RDEB are not well understood and there are limited therapeutic options. RDEB-cSCC arises through the accumulation of genetic mutations; however, previous work analyzing gene expression profiles have not been able to explain its aggressive nature. Therefore, we generated a model to study RDEB-cSCC development using cellular reprograming and re-differentiation technology. We compared RDEB-cSCC to cSCC that were first reprogrammed into induced pluripotent stem cells (RDEB-cSCC-iPSC) and then differentiated back to keratinocytes (RDEB-cSCC-iKC). The RDEB-cSCC-iKC cell population had reduced proliferative capacities in vitro and in vivo, suggesting that reprogramming and re-differentiation leads to functional changes. Finally, we performed RNA-seq analysis for RDEB-cSCC, RDEB-cSCC-iPSC, and RDEB-cSCC-iKC and identified different gene expression signatures between these cell populations. Taken together, this cell culture model offers a valuable tool to study cSCC and provides a novel way to identify potential therapeutic targets for RDEB-cSCC.

scholarly journals Gene expression analysis of head and neck squamous cell carcinoma survival and recurrence

Oncotarget ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 547-555 ◽  
Author(s):  
Xu Zhi ◽  
Katarzyna Lamperska ◽  
Paweł Golusinski ◽  
Nicholas J. Schork ◽  
Lukasz Luczewski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Expression Analysis ◽  
Squamous Cell ◽  
Gene Expression Analysis ◽  
Neck Squamous Cell Carcinoma
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