scholarly journals A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Sissi Miguel ◽  
Guillaume Legrand ◽  
Léonor Duriot ◽  
Marianne Delporte ◽  
Barbara Menin ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Ipomoea Batatas ◽  
Efficient Production ◽  
Coffee Bean ◽  
The Road ◽  
Dicaffeoylquinic Acid ◽  
Low Efficiency ◽  
Green Coffee Bean Extract ◽  
Gdsl Lipase

AbstractThe synthesis of 3,5-dicaffeoylquinic acid (3,5-DiCQA) has attracted the interest of many researchers for more than 30 years. Recently, enzymes belonging to the BAHD acyltransferase family were shown to mediate its synthesis, albeit with notably low efficiency. In this study, a new enzyme belonging to the GDSL lipase-like family was identified and proven to be able to transform chlorogenic acid (5-O-caffeoylquinic acid, 5-CQA, CGA) in 3,5-DiCQA with a conversion rate of more than 60%. The enzyme has been produced in different expression systems but has only been shown to be active when transiently synthesized in Nicotiana benthamiana or stably expressed in Pichia pastoris. The synthesis of the molecule could be performed in vitro but also by a bioconversion approach beginning from pure 5-CQA or from green coffee bean extract, thereby paving the road for producing it on an industrial scale.

scholarly journals Author Correction: A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Sissi Miguel ◽  
Guillaume Legrand ◽  
Léonor Duriot ◽  
Marianne Delporte ◽  
Barbara Menin ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Ipomoea Batatas ◽  
Efficient Production ◽  
Gdsl Lipase

A Correction to this paper has been published: https://doi.org/10.1038/s42003-020-01488-x

scholarly journals Introduction pages

2021 ◽  
Vol 49 (1) ◽  
pp. 12318
Author(s):  
Radu E. SESTRAS
Keyword(s):  
Ipomoea Batatas ◽  
Ex Situ ◽  
Alternanthera Philoxeroides ◽  
Efficient Production ◽  
Non Coding Rna ◽  
Geographical Regions ◽  
Antioxidant Characteristics ◽  
Viral Diagnosis ◽  
The Impact

Notulae Botanicae Horti Agrobotanici Cluj-Napoca (NBHA): The papers published in Issue 1, Volume 49, 2021 represent new exciting researches in different topics of life science, respectively in plant science, horticulture, agronomy and crop science. Among the interesting articles we invite you to find news about: Comparative transcriptome analysis reveals gene network regulation of TGase-induced thermotolerance in tomato; Characterization the coding and non-coding RNA components in the transcriptome of invasion weed Alternanthera philoxeroides; Morphometric analysis and sequence related amplified polymorphism determine genetic diversity in Salvia species; Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam.; Influence of fertilizer and salicylic acid treatments on growth, physiological, and antioxidant characteristics in green and red Perilla frutescens varieties; In-vitro evaluation of antioxidant and antiradical potential of successive extracts, semi-purified fractions and biosynthesized silver nanoparticles of Rumex vesicarius; Zoophagous entomofauna and entomopathogenic agents reported on Cydalima perspectalis (Walker, 1859) in north-western of Romania; Vegetative propagation and ex-situ conservation of Acantholimon androsaceum and Limonium chersonesum, two promising local endemics of Crete (Greece) available for floricultural and pharmaceutical sustainable exploitation; Quality attributes during maturation of ‘Golden Delicious’ and ‘Red Delicious’ apples grown in two geographical regions with different environmental conditions, etc. The Impact Factor communicated by ISI Clarivate, June 29, 2020, is IF 2019 = 1.168 (position 149 of 234 journals, Q3 in Plant Sciences). The metrics in Scopus – Elsevier (June 22, 2020): CiteScore 1.40 (#43/84 in Horticulture); SJR 0.35 - Q2, #41/90 in Horticulture (SJR Scimago Journal).   ---------------------------------------------------------------------------------- Announcement From Volume 49, Issue 1, 2021, Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal will use article numbers in place of the traditional method of continuous pagination through the volume. This step helps us to maintain a rapid, efficient production process by being able to define pagination as soon as a paper is accepted. For papers that use article numbers, the page number of full-text articles will start from 1 to the last page and the citation needs only to list the article number. The journal will continue to appear quarterly, as before, with four annual numbers (see Publication Frequency).

scholarly journals Cell-Free Metabolic Engineering: Recent Developments and Future Prospects

2019 ◽  
Vol 2 (2) ◽  
pp. 33 ◽  
Author(s):  
Hye Jin Lim ◽  
Dong-Myung Kim
Keyword(s):  
Metabolic Engineering ◽  
Raw Material ◽  
Efficient Production ◽  
Recent Developments ◽  
Potential Benefits ◽  
Potential Alternative ◽  
Low Efficiency ◽  
Metabolic Activities ◽  
High Conversion Yield

Due to the ongoing crises of fossil fuel depletion, climate change, and environmental pollution, microbial processes are increasingly considered as a potential alternative for cleaner and more efficient production of the diverse chemicals required for modern civilization. However, many issues, including low efficiency of raw material conversion and unintended release of genetically modified microorganisms into the environment, have limited the use of bioprocesses that rely on recombinant microorganisms. Cell-free metabolic engineering is emerging as a new approach that overcomes the limitations of existing cell-based systems. Instead of relying on metabolic processes carried out by living cells, cell-free metabolic engineering harnesses the metabolic activities of cell lysates in vitro. Such approaches offer several potential benefits, including operational simplicity, high conversion yield and productivity, and prevention of environmental release of microorganisms. In this article, we review the recent progress in this field and discuss the prospects of this technique as a next-generation bioconversion platform for the chemical industry.

scholarly journals Anti-austeric Activity of Phenolic Constituents of Seeds of Arctium lappa

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yasuhiro Tezuka ◽  
Keiichi Yamamoto ◽  
Suresh Awale ◽  
Feng Li ◽  
Satoshi Yomoda ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cytotoxic Activity ◽  
Chlorogenic Acid ◽  
Human Pancreatic Cancer ◽  
Arctium Lappa ◽  
Dicaffeoylquinic Acid ◽  
Phenolic Constituents ◽  
Pure Compounds ◽  
F 14

From seeds of Arctium lappa L. (Asteraceae) we obtained arctigenin (1), arctiin (2), chlorogenic acid (3), 4,5-dicaffeoylquinic acid (4), 3,5-dicaffeoylquinic acid (5), 3,4-dicaffeoylquinic acid (6), matairesinol (11), isolappaol A (12), lappaol F (14), and lappaol B (15), together with 1:1 mixtures of isolappaol C (7) and lappaol C (8), arctignan E (9) and arctignan D (10), and 12 and lappaol A (13), while 3,3′,4′-tri- O-demethylarctigenin (16), 3,3′-di- O-demethyl-4′-dehydroxyarctigenin (17), and 3- O-demethylarctigenin (18) were obtained by anaerobic microbiological metabolism of 1. Then, we evaluated the in vitro preferential cytotoxic activity of these pure compounds and 1:1 mixtures, together with enterodiol (19) and enterolactone (20), against human pancreatic cancer PANC-1 cells in nutrient-deprived medium (NDM). Among them, 1 and 18 showed potent activity, with PC50 values of 1.75 and 4.38 μ M, respectively, while 11, 15, and 17 showed mild activity with PC50 values of 31.1, 30.9, and 38.7 μ/M, respectively. By comparing their structures and PC50 values, the following structural moieties could be concluded to be important for the preferential cytotoxicity of 1: 1) the 3-hydroxy-4-methoxyphenyl group at the 2-position on the γ-butyrolactone ring, 2) the less polar substituent at the 3-position on the γ-butyrolactone ring, and 3) the γ-butyrolactone ring.

Sweetpotato genetic resources: today and tomorrow

2019 ◽  
Author(s):  
Robert L. Jarret ◽  
◽  
Noelle L. Anglin ◽  
David Ellis ◽  
Arthur Villordon ◽  
...  
Keyword(s):  
Ipomoea Batatas ◽  
Global Scale ◽  
Crop Wild Relatives ◽  
International Treaties ◽  
Important Food ◽  
Germplasm Storage ◽  
Archaeological Data ◽  
The Status ◽  
Generation Sequencing

The sweetpotato (Ipomoea batatas (L.) Lam.) is the sixth most important food crop on a global scale. While China accounts for about 80% of global production, Nigeria, Uganda, Indonesia and Tanzania are also large producers of sweetpotato. The chapter examines the origin and dispersal of sweetpotato, including archaeological data for the early distribution of the crop, before moving on to considering its general botany. The chapter considers in vitro germplasm storage in sweetpotato genebanks, as well as issues of quality control. The chapter looks at the importance of managing sweetpotato crop wild relatives (CWR) and examines plant quarantine and phytosanitary issues and the status of genebanks under international treaties. The chapter considers a number of specific issues associated with sweetpotato germplasm. Finally, the chapter looks at the application of next-generation sequencing to sweetpotato and its CWR, before looking ahead to future trends in this area.

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris

1985 ◽  
Vol 5 (5) ◽  
pp. 1111-1121
Author(s):  
S B Ellis ◽  
P F Brust ◽  
P J Koutz ◽  
A F Waters ◽  
M M Harpold ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeasts ◽  
Sequence Analyses ◽  
Yeast Pichia Pastoris ◽  
Oxidation Of Methanol ◽  
Regulated Expression

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

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