scholarly journals High throughput estimates of Wolbachia, Zika and chikungunya infection in Aedes aegypti by near-infrared spectroscopy to improve arbovirus surveillance

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Lilha M. B. Santos ◽  
Mathijs Mutsaers ◽  
Gabriela A. Garcia ◽  
Mariana R. David ◽  
Márcio G. Pavan ◽  
...  

AbstractDeployment of Wolbachia to mitigate dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) transmission is ongoing in 12 countries. One way to assess the efficacy of Wolbachia releases is to determine invasion rates within the wild population of Aedes aegypti following their release. Herein we evaluated the accuracy, sensitivity and specificity of the Near Infrared Spectroscopy (NIRS) in estimating the time post death, ZIKV-, CHIKV-, and Wolbachia-infection in trapped dead female Ae. aegypti mosquitoes over a period of 7 days. Regardless of the infection type, time post-death of mosquitoes was accurately predicted into four categories (fresh, 1 day old, 2–4 days old and 5–7 days old). Overall accuracies of 93.2, 97 and 90.3% were observed when NIRS was used to detect ZIKV, CHIKV and Wolbachia in dead Ae. aegypti female mosquitoes indicating NIRS could be potentially applied as a rapid and cost-effective arbovirus surveillance tool. However, field data is required to demonstrate the full capacity of NIRS for detecting these infections under field conditions.

2021 ◽  
Vol 271 ◽  
pp. 03067
Author(s):  
Xiaohong He ◽  
Zhihong Song ◽  
Haifei Shang ◽  
Silang Yang ◽  
Lujing Wu ◽  
...  

Currently, the laboratory diagnostic tests available for HIV-1 viral infection are mainly based on serological testing which relies on enzyme-linked immunosorbent assay (ELISA) for blood HIV antigen detection and reverse transcription polymerase chain reaction (RT-PCR) for HIV specific RNA sequence identification. However, these methods are expensive and time-consuming, and suffer from false positive and/or false negative results. Thus, there is an urgent need for developing a cost effective, rapid and accurate diagnostic method for HIV-1 infection. In order to reduce the barriers for effective diagnosis, a near-infrared spectroscopy (NIR) method was used to detect the HIV-1 virus in human serum, specifically, three absorption peaks with dose-dependent at 1582nm, 1810nm and 2363nm were found by multiple FBiPLSR test analysis for HIV-nano and HIV-EGFP, but not for MLV. Therefore, we recommend the use of 1582nm, 1810nm and 2363nm as the characteristic spectrum peak, for early screening and rapid diagnosis of serum HIV.


2021 ◽  
Vol 7 ◽  
pp. 2513826X2110511
Author(s):  
Spencer Yakaback ◽  
Carmen Webb ◽  
Claire Temple-Oberle

Paramedian forehead flaps (PMFFs) are commonly performed procedures for the reconstruction of complex nasal defects and require multiple stages predicated on when the interpolated pedicle is no longer required to provide perfusion to the neo-nose. As the presence of the pedicle is disfiguring, the flap is therefore divided ideally as soon as safely possible, but that determination is challenging. Recently, a novel device that uses near-infrared spectroscopy (NIRS) to measure tissue oxygen saturation has been developed and may provide an accurate and cost-effective tool to assess tissue perfusion. Here we present the use of this technology in the management of 2 patients who underwent successful oncologic nasal reconstructions using PMFFs. Using the device as a clinical adjunct, we measured tissue oxygen saturation values in the flaps before and after pedicle division, which assisted us in deciding when to safely divide the flaps, as well as with post-division management, particularly when a low tissue oxygen saturation reading ultimately necessitated the use of nitroglycerine past to improve flap perfusion in one of our patients. Interestingly, the values we recorded corresponded well to previously published cut-off values for adequate tissue perfusion, which have to date only been reported for free flaps. To our knowledge, this is the first description of the use of NIRS in the division of PMFFs, which we overall found to be a useful and reliable adjunct to a clinical examination in assessing flap neovascularization.


2002 ◽  
Vol 10 (4) ◽  
pp. 279-287 ◽  
Author(s):  
G. Lachenal ◽  
I. Stevenson

The feasibility of using near infrared spectroscopy as a sensitive technique to follow the influence of γ-irradiation upon ageing of different EPDM (Ethylene Propylene Diene Monomer) elastomers has been evaluated. Minor differences can be observed between the spectra of non-irradiated and irradiated materials (for total integrated doses from 50 to 450 kGy using a dose rate of 1 kGy h−1 under oxygen flow). Applying a principal component analysis (PCA) in the spectral range 5500–4500 cm−1, the discrimination of polymers irradiated at different dose levels can be quickly performed and a spatial “random” separation by irradiation doses can be obtained up to 400 kGy using score plots PC1/PC2. Moreover, when spectral ranges such as 5000–4500 cm−1 or 7090–6980 cm−1 are used, scores of PC1 contain enough information for discrimination and classification by increasing irradiation doses. According to PCA analysis, the improvements brought by chemical modifications of the initial EPDM (antioxidant) seem to be inefficient as soon as the lowest irradiation doses (50 kGy) are applied. This preliminary study opens up a cost-effective opportunity for the in situ and non-destructive monitoring of polymer degradations in nuclear power plants.


1998 ◽  
Vol 38 (7) ◽  
pp. 697 ◽  
Author(s):  
G. D. Batten

Summary. International Standards Committees have formally accepted methods using near infrared spectroscopy for the analysis of protein, moisture and hardness in grains, and protein, acid detergent fibre and moisture in forages. In addition, near infrared spectroscopy is used world-wide for the routine analysis of many constituents in various tissues of many plant species. The reasons for near infrared spectroscopy being adopted as the preferred analytical method in many laboratories include: minimal sample preparation is needed, analysis time is short, it is cost effective to analyse a single sample or large batches of samples, several constituents can be determined simultaneously, the samples are not destroyed during analysis, neither a laboratory nor a skilled operator are required for routine analyses, the use of hazardous chemical reagents is eliminated, and, depending on the method, the results are usually more precise and can be more accurate than, as accurate as, or of acceptable accuracy, when compared with the method usually employed. It is the responsibility of the analyst to choose the most appropriate analytical technique and to follow standard procedures in order to obtain accurate and precise results from routine analysis. This paper summarises some of the applications of near infrared spectroscopy which are being used to analyse plant samples and lists some of the criteria which can be used to decide if near infrared spectroscopy is the most appropriate technique.


2015 ◽  
Vol 92 (5) ◽  
pp. 1070-1075 ◽  
Author(s):  
Kelly Liebman ◽  
Floyd Dowell ◽  
Audrey Lenhart ◽  
Lucrecia Vizcaino ◽  
Isabel Swamidoss ◽  
...  

Molecules ◽  
2017 ◽  
Vol 22 (5) ◽  
pp. 851 ◽  
Author(s):  
Eva Toledo-Martín ◽  
Rafael Font ◽  
Sara Obregón-Cano ◽  
Antonio De Haro-Bailón ◽  
Myriam Villatoro-Pulido ◽  
...  

2016 ◽  
Vol 10 (6) ◽  
pp. e0004759 ◽  
Author(s):  
Maggy T. Sikulu-Lord ◽  
Marta F. Maia ◽  
Masabho P. Milali ◽  
Michael Henry ◽  
Gustav Mkandawile ◽  
...  

2012 ◽  
Vol 479-481 ◽  
pp. 1772-1776 ◽  
Author(s):  
Zhong Yang ◽  
Bin Lv ◽  
Yue Jin Fu

Rosewood is a kind of precious wood. Accurate wood species identification is a time consuming task for highly trained human experts. The development of cost effective techniques for automatic wood identification is a desirable goal. Wood color is one of the crucial factors to identify wood species. The color parameters (CIE L*, a* and b*) of eight rosewoods and the relationship between near infrared spectroscopy (NIR) and the color parameters were investigated in this paper. The results showed there was significant correlation between near infrared spectroscopy and color parameters. The correlation coefficients between laboratory-measured and NIR-predicted L*, a* and b* values were 0.93~0.99. The results will be helpful to develop a new method of rosewood identification through near infrared spectroscopy coupled with multivariate data analysis.


2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Simon Juric ◽  
Vojko Flis ◽  
Matjaz Debevc ◽  
Andreas Holzinger ◽  
Borut Zalik

Excessive venipunctures are both time- and resource-consuming events, which cause anxiety, pain, and distress in patients, or can lead to severe harmful injuries. We propose a low-cost mobile health solution for subcutaneous vein detection using near-infrared spectroscopy, along with an assessment of the current state of the art in this field. The first objective of this study was to get a deeper overview of the research topic, through the initial team discussions and a detailed literature review (using both academic and grey literature). The second objective, that is, identifying the commercial systems employing near-infrared spectroscopy, was conducted using the PubMed database. The goal of the third objective was to identify and evaluate (using the IEEE Xplore database) the research efforts in the field of low-cost near-infrared imaging in general, as a basis for the conceptual model of the upcoming prototype. Although the reviewed commercial devices have demonstrated usefulness and value for peripheral veins visualization, other evaluated clinical outcomes are less conclusive. Previous studies regarding low-cost near-infrared systems demonstrated the general feasibility of developing cost-effective vein detection systems; however, their limitations are restricting their applicability to clinical practice. Finally, based on the current findings, we outline the future research direction.


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