scholarly journals Towards organoid culture without Matrigel

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Mark T. Kozlowski ◽  
Christiana J. Crook ◽  
Hsun Teresa Ku
Keyword(s):  
Developmental Biology ◽  
Genetic Disorders ◽  
Organ Function ◽  
Organoid Culture ◽  
Decellularized Extracellular Matrix ◽  
Wide Range ◽  
Mouse Sarcoma ◽  
Specific Factors ◽  
Sarcoma Cells ◽  
Cellular Aggregates

AbstractOrganoids—cellular aggregates derived from stem or progenitor cells that recapitulate organ function in miniature—are of growing interest in developmental biology and medicine. Organoids have been developed for organs and tissues such as the liver, gut, brain, and pancreas; they are used as organ surrogates to study a wide range of questions in basic and developmental biology, genetic disorders, and therapies. However, many organoids reported to date have been cultured in Matrigel, which is prepared from the secretion of Engelbreth-Holm-Swarm mouse sarcoma cells; Matrigel is complex and poorly defined. This complexity makes it difficult to elucidate Matrigel-specific factors governing organoid development. In this review, we discuss promising Matrigel-free methods for the generation and maintenance of organoids that use decellularized extracellular matrix (ECM), synthetic hydrogels, or gel-forming recombinant proteins.

scholarly journals Cardiovascular Outcomes in Patients With Mitochondrial Disease in the United States: A Propensity Score Analysis

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Tran Nguyen ◽  
Talal Alzahrani ◽  
Joseph Krepp ◽  
Gurusher Panjrath
Keyword(s):  
Heart Failure ◽  
Mitochondrial Disease ◽  
Cardiovascular Events ◽  
Propensity Score Analysis ◽  
Genetic Disorders ◽  
Systolic Heart Failure ◽  
The United States ◽  
Major Adverse Cardiovascular Events ◽  
Hospital Death ◽  
Wide Range

Mitochondrial disease comprises a wide range of genetic disorders caused by mitochondrial dysfunction. Its rarity, however, has limited the ability to assess its effects on clinical outcomes. To evaluate this relationship, we collected data from the 2016 National Inpatient Sample, which includes data from >7 million hospital stays. We identified 705 patients (mean age, 22 ± 20.7 yr; 54.2% female; 67.4% white) whose records included the ICD-10-CM code E88.4. We also identified a propensity-matched cohort of 705 patients without mitochondrial disease to examine the effect of mitochondrial disease on major adverse cardiovascular events, including all-cause in-hospital death, cardiac arrest, and acute congestive heart failure. Patients with mitochondrial disease were at significantly greater risk of major adverse cardiovascular events (odds ratio [OR]=2.42; 95% CI, 1.29–4.57; P=0.005), systolic heart failure (OR=2.37; 95% CI, 1.08–5.22; P=0.027), and all-cause in-hospital death (OR=14.22; 95% CI, 1.87–108.45; P<0.001). These findings suggest that mitochondrial disease significantly increases the risk of inpatient major adverse cardiovascular events.

Decellularized extracellular matrix hydrogels for human airway organoid culture

2021 ◽  
Author(s):  
S Tas ◽  
D A Bölükbas ◽  
H N Alsafadi ◽  
I A Da Silva ◽  
M M De Santis ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Organoid Culture ◽  
Human Airway ◽  
Decellularized Extracellular Matrix

Congenital disorders

2018 ◽  
pp. 160-163
Author(s):  
Sowmiya Moorthie
Keyword(s):  
Developmental Disorders ◽  
Genetic Disorders ◽  
Holistic Approach ◽  
Congenital Disorders ◽  
Mortality And Morbidity ◽  
Specialist Services ◽  
Wide Range ◽  
Effective Delivery ◽  
The Life Course ◽  
Preventative Services

Congenital disorders encompass a wide range of conditions (e.g. genetic disorders, foetal disease, and developmental disorders) that occur before birth and are an important contributor to mortality and morbidity worldwide. Congenital disorders can be identified at different life stages and effective health services take a holistic approach to their care and prevention. This involves both population health and specialist services across the life course. Systematic collection of data on the types, prevalence, severity, and outcomes of congenital disorders, along with analysis and interpretation of data helps to inform appropriate planning of care and preventative services and activities. Important concepts in relation to congenital disorders, prevention activities, and key challenges to their effective delivery are described in this chapter.

Mouse G2-GPI AChE is processed as a membrane-bound ectoenzyme in transfected mouse sarcoma cells but is not a homophilic adhesion molecule

1996 ◽  
Vol 210 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Marc Guenneugues ◽  
Martine Verdière-Sahuqué ◽  
Rim Amouri ◽  
Danièle Goudou ◽  
François Rieger
Keyword(s):  
Adhesion Molecule ◽  
Mouse Sarcoma ◽  
Membrane Bound ◽  
Sarcoma Cells ◽  
Homophilic Adhesion

scholarly journals Anesthesia outside the Operating Room in a Patient with Mitochondrial Disease

2020 ◽  
Vol 2020 ◽  
pp. 1-3
Author(s):  
Alejandra Fadrique-Fuentes ◽  
Beatriz Martínez-Rafael ◽  
Rodrigo Poves-Álvarez ◽  
Estefanía Gómez-Pesquera
Keyword(s):  
Mitochondrial Dysfunction ◽  
Operating Room ◽  
Mitochondrial Disease ◽  
Genetic Disorders ◽  
Metabolic Balance ◽  
Anesthetic Agents ◽  
Wide Range

Mitochondrial dysfunction comprehends a wide range of genetic disorders. These patients’ precarious metabolic balance makes its management difficult. Furthermore, the same systems affected by mitochondrial disease can be altered by many of the frequently used anesthetic agents. Each patient has to be evaluated individually according to their comorbidities and anesthetic requirements.

scholarly journals Cichorins D–F: Three New Compounds from Cichorium intybus and Their Biological Effects

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4160
Author(s):  
Muhammad Farooq Khan ◽  
Fahd A. Nasr ◽  
Omar M. Noman ◽  
Nouf Abdulaziz Alyhya ◽  
Iftikhar Ali ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Biological Effects ◽  
2D Nmr ◽  
Cichorium Intybus ◽  
Traditional Medicines ◽  
Wide Range ◽  
Sarcoma Cells ◽  
New Compounds

Cichorium intybus L., (chicory) is employed in various traditional medicines to treat a wide range of diseases and disorders. In the current investigation, two new naphthalane derivatives viz., cichorins D (1) and E (2), along with one new anthraquinone cichorin F (3), were isolated from Cichorium intybus. In addition, three previously reported compounds viz., β-sitosterol (4), β-sitosterol β-glucopyranoside (5), and stigmasterol (6) were also isolated from Cichorium intybus. Their structures were established via extensive spectroscopic data, including 1D (1H and 13C) and 2D NMR (COSY, HSQC and HMBC), and ESIMS. Cichorin E (2) has a weak cytotoxic effect on breast cancer cells (MDA-MB-468: IC50: 85.9 µM) and Ewing’s sarcoma cells (SK-N-MC: IC50: 71.1 µM); cichorin F (3) also illustrated weak cytotoxic effects on breast cancer cells (MDA-MB-468: IC50: 41.0 µM and MDA-MB-231: IC50: 45.6 µM), and SK-N-MC cells (IC50: 71.9 µM). Moreover compounds 1–3 did not show any promising anthelmintic effects.

Strategies of cities in globalised interurban competition: The locational policies framework

Urban Studies ◽  
2017 ◽  
Vol 55 (12) ◽  
pp. 2703-2720 ◽  
Author(s):  
David Kaufmann ◽  
Tobias Arnold
Keyword(s):  
Economic Geography ◽  
Analytical Framework ◽  
Added Value ◽  
Global Markets ◽  
Tax System ◽  
Wide Range ◽  
Two Cities ◽  
Specific Factors ◽  
Knowledge Intensive ◽  
National Tax

Globalised interurban competition affects cities of various sizes and cities in various locations. Cities have to find ways to position themselves in global markets by formulating locational policies. To capture this wide range of policies, this paper develops an analytical framework of locational policies that is interdisciplinary informed by theories of economic geography and political science. We compare the cities of Lucerne and Ulm to illustrate the added value of the locational policies framework. We found that these two cities feature very different locational policies agendas. By employing a neo-institutional lens, we suggest that place-specific factors enable and constrain the formulation of locational policies. We outline three possible venues to tentatively explain these different locational policies, namely the economic sector mix, the national tax system, and politics. Beyond these empirical findings, this paper shows that the locational policies framework is able to capture a wide range of policies that aim to enhance the competitiveness of a city. Thus, the locational policies framework is a tool that can be used to reveal how cities face the globalised, and increasingly knowledge-intensive, interurban competition.

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