Monte Carlo simulation for reference tissue-based linear analysis of [11C]MP4A AND [11C]MP4P: Assessment of optimal regions and optimization for precise measurement of brain AChE activity

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S644-S644
Author(s):  
Koichi Sato ◽  
Kiyoshi Fukushi ◽  
Hitoshi Shinotoh ◽  
Shin-ichiro Nagatsuka ◽  
Noriko Tanaka ◽  
...  
1960 ◽  
Vol 198 (3) ◽  
pp. 657-658 ◽  
Author(s):  
F. W. Kinard ◽  
M. G. Hay

Rats receiving daily administrations of ethanol for 10–12 weeks became demonstrably resistant to intoxicating effects of the alcohol by the 9th week. The increased resistance to the effects of alcohol was not accompanied by increased oxidation of alcohol in the intact animal or by increased oxidation of ethanol in liver homogenate. Brain AChE activity is reduced to a statistically demonstrable but functionally insignificant degree by administration of alcohol whether the rat is receiving its first dose or whether it has become tolerant to the intoxicating effects of alcohol. The development of tolerance to ethanol appears to be unrelated to the rate of oxidation of alcohol in either the intact animal or in liver tissue and does not appear related to changes in brain AChE activity.


2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S592-S592
Author(s):  
Noriko Tanaka ◽  
Hajime Matsuura ◽  
Hitoshi Shinotoh ◽  
Sigeki Hirano ◽  
Yasukazu Sato ◽  
...  

Author(s):  
Charles G. Glancy ◽  
Kenneth W. Chase

Abstract Linear analysis and Monte Carlo simulation are two well-established methods for statistical tolerance analysis of mechanical assemblies. Both methods have advantages and disadvantages. The Linearized Method, a form of linear analysis, provides fast analysis, tolerance allocation, and the capability to solve closed loop constraints. However, the Linearized Method does not accurately approximate nonlinear geometric effects or allow for non-normally distributed input or output distributions. Monte Carlo simulation, on the other hand, does accurately model nonlinear effects and allow for non-normally distributed input and output distributions. Of course, Monte Carlo simulation can be computationally expensive and must be re-run when any input variable is modified. The second-order tolerance analysis (SOTA) method attempts to combine the advantages of the Linearized Method with the advantages of Monte Carlo simulation. The SOTA method applies the Method of System Moments to implicit variables of a system of nonlinear equations. The SOTA method achieves the benefits of speed, tolerance allocation, closed-loop constraints, non-linear geometric effects and non-normal input and output distributions. The SOTA method offers significant benefits as a nonlinear analysis tool suitable for use in design iteration. A comparison was performed between the Linearized Method, Monte Carlo simulation, and the SOTA method. The SOTA method provided a comparable nonlinear analysis to Monte Carlo simulation with 106 samples. The analysis time of the SOTA method was comparable to the Linearized Method.


1985 ◽  
Vol 33 (7) ◽  
pp. 631-641 ◽  
Author(s):  
C D Ross ◽  
D A Godfrey

Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities were assayed in samples dissected from sagittal sections through rat superior colliculus. The magnitude of ChAT activity was about half to equal that found in rat whole brain in all layers except stratum griseum intermediale, where the average activity was higher than whole brain. AChE activity was three to four times that found in rat whole brain in superficial layers and about the same as average brain in deeper layers, except in the statum griseum intermediale, where the average activity was about twice whole brain. Rostral-caudal gradients in both ChAT and AChE activities occurred in stratum griseum intermediale, with activities in the caudal region of some animals as high as four times those in the rostral. ChAT activity in samples associated with locations of patches or spots of AChE staining product in stratum griseum intermediale was significantly higher than in samples from "nonpatch" regions. Results are discussed relative to inputs into the colliculus, whose terminations may correlate in location with the distributions of the enzyme activities.


1993 ◽  
Vol 27 (7-8) ◽  
pp. 465-472
Author(s):  
A. Yawetz ◽  
R. Manelis ◽  
A. Gasith

In the tilapia as well as in the carp, gill acetylcholinesterase (AChE) was more sensitive than brain AChE to organophosphorous (OP) and carbamate (CB) compounds, and the threshold levels for residue detection by the biochemical method were lower in the gills, compared to the brain. The sensitivity of tilapia gill AChE to paraoxon was extremely high, and enabled detection of paraoxon residues in the gills at the ppb level. The carp showed higher resistance than the tilapia for poisoning by both the OP compound parathion and the carbamate methomyl. The residual post-mortem brain and gill AChE activities from tilapia killed by either parathion or methomyl poisoning were low, and could easily be differentiated from brain AChE activity in the control ish, which remained normal until 18 hours after death, providing that fish were held at temperature up to 10°C. Detection of poisoning with OP and CB compounds, in fish that are being marketed, is of extreme importance, especially in Lake Kinneret, where illegal fishing with insecticides occurs occasionally.


2000 ◽  
Vol 07 (05n06) ◽  
pp. 607-611 ◽  
Author(s):  
MASAHIDE SATO ◽  
MAKIO UWAHA ◽  
YUKIO SAITO

We study the effect of permeability on the two types of step instabilities, bunching and wandering. When the step is permeable for adatom diffusion, the wandering instability occurs with step-down drift and the bunching instability occurs with step-up drift. In contrast, when the step is impermeable for adatom diffusion, both the wandering and the bunching instabilities occur with step-down drift. We derive the linear amplification rates of step fluctuation and the critical drift velocities to induce the instabilities. The results of the linear analysis are tested by Monte Carlo simulation.


2020 ◽  
pp. 36-42
Author(s):  
Badruddeen N/A ◽  
Juber Akhtar ◽  
Muhammad Arif ◽  
Mohammad Irfan Khan ◽  
Md. Mujahid ◽  
...  

Background. There is not much evidence illustrating that statins could be responsible for memory loss or dementia, although increased exposure to statins has been reported to cause cognitive side effects. The present study investigated the effect of lovastatin in combination with rivastigmine on cognitive function as well as brain acetylcholinesterase (AChE) activity in normal mice. Methods. The mice were categorized into four groups, and they were treated with normal saline, lovastatin, rivastigmine, and the combination of lovastatin and rivastigmine, respectively, by oral administration for 60 days. The treatment effect on cognitive functions was assessed by behavioral tests, namely, the passive avoidance test and spontaneous alternation test, as well as the measurement of brain AChE activity by Ellman’s method. Results. In this study, a significant reduction (P < 0.01) of brain AChE activity and positive effects (P < 0.01) on cognitive functions was observed in mice treated with the combination of lovastatin and rivastigmine as compared to rivastigmine alone. However, no significant differences (P < 0.05) were observed on brain AChE activity as well as cognitive functions in mice treated with lovastatin when compared with those treated with normal saline. Conclusion. This study suggested that lovastatin did not contribute to any improvements in cognitive functions and brain AChE activity, but it potentiated the effect of rivastigmine.


2012 ◽  
Vol 33 (3) ◽  
pp. 469-475 ◽  
Author(s):  
Vanessa Maynart Pereira ◽  
Josiane Woutheres Bortolotto ◽  
Luiza Wilges Kist ◽  
Mariana Barbieri de Azevedo ◽  
Rachel Seemann Fritsch ◽  
...  

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