An improved method for determination of ethyl carbamate in Korean traditional rice wine

2001 ◽  
Vol 26 (6) ◽  
pp. 363-368 ◽  
Author(s):  
I-S Woo ◽  
I-H Kim ◽  
U-J Yun ◽  
S-K Chung ◽  
I-K Rhee ◽  
...  
Keyword(s):  
Ethyl Carbamate ◽  
Rice Wine ◽  
Improved Method ◽  
Traditional Rice

Development of an indirect ELISA for the determination of ethyl carbamate in Chinese rice wine

2017 ◽  
Vol 950 ◽  
pp. 162-169 ◽  
Author(s):  
Lin Luo ◽  
Hong-Tao Lei ◽  
Jin-Yi Yang ◽  
Gong-Liang Liu ◽  
Yuan-Ming Sun ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Ethyl Carbamate ◽  
Rice Wine ◽  
Chinese Rice Wine

Determination of Ethyl Carbamate (EC) by GC-MS and Characterization of Aroma Compounds by HS-SPME-GC-MS During Wine Frying Status in Hakka Yellow Rice Wine

2016 ◽  
Vol 10 (6) ◽  
pp. 2068-2077 ◽  
Author(s):  
Weidong Bai ◽  
Shuangge Sun ◽  
Wenhong Zhao ◽  
Min Qian ◽  
Xiaoyan Liu ◽  
...  
Keyword(s):  
Ethyl Carbamate ◽  
Aroma Compounds ◽  
Rice Wine

Determination of Ethyl Carbamate in Chinese Yellow Rice Wine by Diatomaceous Earth Extraction and GC/MS Method

2015 ◽  
Vol 98 (3) ◽  
pp. 834-838 ◽  
Author(s):  
Pinggu Wu ◽  
Liqun Zhang ◽  
Xianghong Shen ◽  
Liyuan Wang ◽  
Yan Zou ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Analytical Method ◽  
Diatomaceous Earth ◽  
Capillary Column ◽  
Internal Standard ◽  
Ethyl Carbamate ◽  
Rice Wine ◽  
Average Recovery

Abstract A sensitive and rapid analytical method based on alkaline diatomaceous earth extraction followed by GC/MS was developed for the quantitative determination of the toxic contaminant ethyl carbamate (EC) in yellow rice wines. The optimal extraction conditions were investigated. With the application of diatomaceous earth extraction, the damage of organic acids to the capillary column was greatly reduced. By using d5-EC as an internal standard for quantitative analysis of EC, the linearity of the calibration curves was good between 10 and 1000 ng/mL. The LOD and LOQ were 1.7 and 5.0 μg/kg, respectively. The spiked level of EC was 5.0–300 μg/kg, and the average recovery of the spikes was between 78.4 and 98.2%, with an RSD between 4.3 and 8.3%. Upon validation by five laboratories when spiked with 50, 100, and 300 μg/kg, the average respective recoveries were 102.9, 102.2, and 98.7% with a RSD between 0.7 and 8.1%. The validation results demonstrated that the method is fast, simple, selective, and suitable for the determination of EC in yellow rice wines.

SERUM TRIIODOTHYRONINE DETERMINATION BY SATURATION ANALYSIS

1973 ◽  
Vol 72 (4) ◽  
pp. 714-726 ◽  
Author(s):  
A. Burger ◽  
B. Miller ◽  
C. Sakoloff ◽  
M. B. Vallotton
Keyword(s):  
Ionic Strength ◽  
Limit Of Detection ◽  
Improved Method ◽  
Accuracy Of Measurement ◽  
Tracer Amount ◽  
The Mean ◽  
Physiological Values ◽  
Hyperthyroid Patients ◽  
Solvent Blank

ABSTRACT An improved method for the determination of serum triiodothyronine (T3) has been developed. After addition of a tracer amount of the hormone, T3 was extracted from 1 ml serum under conditions of pH and ionic strength which favoured T3 extraction (89%) over thyroxine (T4) extraction (58%). Chromatography of the extracted material on Sephadex LH-20 separated T3 completely from residual T4. The T3 eluate was dried, then re-dissolved in 0.5 ml NaOH 0.04 n. To 0.2 ml duplicate aliquots, a standard amount of TBG was added for the competitive protein analysis. After one hour incubation at 4°C, separation of bound from free T3 was achieved on small Sephadex G-25 columns. Overall recovery was 67 ± 10.8% and correction for the loss was made. The solvent blank was 37 ± 27 (sd) ng/100 ml. Accuracy of measurement of known quantities of T3 added to serum was 98.4%. The coefficient of variation within the assay was 6.2% and between the assays it was 11.4%. The limit of detection (0.1 ng) corresponded to a concentration of 25 ng/100 ml. T4 added to serum did not interfere with T3 determination until high non-physiological values were reached. The mean ± sd serum T3 in 54 euthyroid subjects was 153 ± 58 ng/100 ml and in 24 hyperthyroid patients it was 428 ±186 ng/100 ml; 4 out of the 24 hyperthyroid values were within 2 sd of the mean euthyroid group. All the values found in the euthyroid group were well above the limit of detection of the method.

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