scholarly journals Robust phase retrieval for high resolution edge illumination x-ray phase-contrast computed tomography in non-ideal environments

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Anna Zamir ◽  
Marco Endrizzi ◽  
Charlotte K. Hagen ◽  
Fabio A. Vittoria ◽  
Luca Urbani ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Phase Contrast ◽  
Phase Retrieval ◽  
X Ray

scholarly journals Functionalized synchrotron in-line phase-contrast computed tomography: a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples

2015 ◽  
Vol 22 (1) ◽  
pp. 143-155 ◽  
Author(s):  
Christian Dullin ◽  
Simeone dal Monego ◽  
Emanuel Larsson ◽  
Sara Mohammadi ◽  
Martin Krenkel ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Lung Tissue ◽  
Alveolar Macrophages ◽  
Phase Contrast ◽  
Phase Retrieval ◽  
Cell Tracking ◽  
Structural Changes ◽  
Mouse Lung ◽  
X Ray ◽  
Novel Approach

Functionalized computed tomography (CT) in combination with labelled cells is virtually non-existent due to the limited sensitivity of X-ray-absorption-based imaging, but would be highly desirable to realise cell tracking studies in entire organisms. In this study we applied in-line free propagation X-ray phase-contrast CT (XPCT) in an allergic asthma mouse model to assess structural changes as well as the biodistribution of barium-labelled macrophages in lung tissue. Alveolar macrophages that were barium-sulfate-loaded and fluorescent-labelled were instilled intratracheally into asthmatic and control mice. Mice were sacrificed after 24 h, lungs were keptin situ, inflated with air and scanned utilizing XPCT at the SYRMEP beamline (Elettra Synchrotron Light Source, Italy). Single-distance phase retrieval was used to generate data sets with ten times greater contrast-to-noise ratio than absorption-based CT (in our setup), thus allowing to depict and quantify structural hallmarks of asthmatic lungs such as reduced air volume, obstruction of airways and increased soft-tissue content. Furthermore, we found a higher concentration as well as a specific accumulation of the barium-labelled macrophages in asthmatic lung tissue. It is believe that XPCT will be beneficial in preclinical asthma research for both the assessment of therapeutic response as well as the analysis of the role of the recruitment of macrophages to inflammatory sites.

X-ray computed tomography in Zernike phase contrast mode at 8 keV with 50-nm resolution using Cu rotating anode X-ray source

2007 ◽  
Vol 222 (11/2007) ◽  
Author(s):  
Andrei Tkachuk ◽  
Fred Duewer ◽  
Hongtao Cui ◽  
Michael Feser ◽  
Steve Wang ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Phase Contrast ◽  
High Efficiency ◽  
Materials Science ◽  
Fresnel Zone ◽  
X Ray ◽  
X Ray Computed ◽  
Structure Height ◽  
Better Than

High-resolution X-ray computed tomography (XCT) enables nondestructive 3D imaging of complex structures, regardless of their state of crystallinity. This work describes a sub-50 nm resolution XCT system operating at 8 keV in absorption and Zernike phase contrast modes based on a commercially available Cu rotating anode laboratory X-ray source. The system utilizes a high efficiency reflective capillary condenser lens and high-resolution Fresnel zone plates with an outermost zone width of 35 nm and 700 nm structure height resulting in a spatial resolution better than 50 nm currently. Imaging a fragment of the solid oxide fuel cells (SOFC) with 50 nm resolution is presented as an application example of the XCT technique in materials science and nanotechnology.

scholarly journals X-ray Phase-Contrast Computed Tomography for Soft Tissue Imaging at the Imaging and Medical Beamline (IMBL) of the Australian Synchrotron

2021 ◽  
Vol 11 (9) ◽  
pp. 4120
Author(s):  
Benedicta D. Arhatari ◽  
Andrew W. Stevenson ◽  
Brian Abbey ◽  
Yakov I. Nesterets ◽  
Anton Maksimenko ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
Medical Imaging ◽  
Breast Imaging ◽  
Phase Contrast ◽  
Phase Retrieval ◽  
Short Exposure ◽  
X Rays ◽  
Ongoing Research ◽  
X Ray

The Imaging and Medical Beamline (IMBL) is a superconducting multipole wiggler-based beamline at the 3 GeV Australian Synchrotron operated by the Australian Nuclear Science and Technology Organisation (ANSTO). The beamline delivers hard X-rays in the 25–120 keV energy range and offers the potential for a range of biomedical X-ray applications, including radiotherapy and medical imaging experiments. One of the imaging modalities available at IMBL is propagation-based X-ray phase-contrast computed tomography (PCT). PCT produces superior results when imaging low-density materials such as soft tissue (e.g., breast mastectomies) and has the potential to be developed into a valuable medical imaging tool. We anticipate that PCT will be utilized for medical breast imaging in the near future with the advantage that it could provide better contrast than conventional X-ray absorption imaging. The unique properties of synchrotron X-ray sources such as high coherence, energy tunability, and high brightness are particularly well-suited for generating PCT data using very short exposure times on the order of less than 1 min. The coherence of synchrotron radiation allows for phase-contrast imaging with superior sensitivity to small differences in soft-tissue density. Here we also compare the results of PCT using two different detectors, as these unique source characteristics need to be complemented with a highly efficient detector. Moreover, the application of phase retrieval for PCT image reconstruction enables the use of noisier images, potentially significantly reducing the total dose received by patients during acquisition. This work is part of ongoing research into innovative tomographic methods aimed at the introduction of 3D X-ray medical imaging at the IMBL to improve the detection and diagnosis of breast cancer. Major progress in this area at the IMBL includes the characterization of a large number of mastectomy samples, both normal and cancerous, which have been scanned at clinically acceptable radiation dose levels and evaluated by expert radiologists with respect to both image quality and cancer diagnosis.

Breast tumor segmentation in high resolution x-ray phase contrast analyzer based computed tomography

2014 ◽  
Vol 41 (11) ◽  
pp. 111902 ◽  
Author(s):  
E. Brun ◽  
S. Grandl ◽  
A. Sztrókay-Gaul ◽  
G. Barbone ◽  
A. Mittone ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Breast Tumor ◽  
Phase Contrast ◽  
Tumor Segmentation ◽  
X Ray

scholarly journals Visualization Ability of Phase-Contrast Synchrotron-Based X-Ray Imaging Using an X-Ray Interferometer in Soft Tissue Tumors

2021 ◽  
Vol 20 ◽  
pp. 153303382110101
Author(s):  
Thet-Thet Lwin ◽  
Akio Yoneyama ◽  
Hiroko Maruyama ◽  
Tohoru Takeda
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
Spatial Resolution ◽  
Phase Contrast ◽  
Soft Tissue Tumors ◽  
X Ray ◽  
3 Dimensional ◽  
Computed Tomography Images ◽  
X Ray Imaging ◽  
X Ray Computed

Phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer provides high sensitivity and high spatial resolution, and it has the ability to depict the fine morphological structures of biological soft tissues, including tumors. In this study, we quantitatively compared phase-contrast synchrotron-based X-ray computed tomography images and images of histopathological hematoxylin-eosin-stained sections of spontaneously occurring rat testicular tumors that contained different types of cells. The absolute densities measured on the phase-contrast synchrotron-based X-ray computed tomography images correlated well with the densities of the nuclear chromatin in the histological images, thereby demonstrating the ability of phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer to reliably identify the characteristics of cancer cells within solid soft tissue tumors. In addition, 3-dimensional synchrotron-based phase-contrast X-ray computed tomography enables screening for different structures within tumors, such as solid, cystic, and fibrous tissues, and blood clots, from any direction and with a spatial resolution down to 26 μm. Thus, phase-contrast synchrotron-based X-ray imaging using an X-ray interferometer shows potential for being useful in preclinical cancer research by providing the ability to depict the characteristics of tumor cells and by offering 3-dimensional information capabilities.

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