Temperature dependence of the nearly diffusion-controlled fluorescence quenching by oxygen of 9,10-dimethylanthracence in liquid solution

2001 ◽  
Vol 3 (17) ◽  
pp. 3696-3700 ◽  
Author(s):  
Masami Okamoto

1977 ◽  
Vol 32 (12) ◽  
pp. 1561-1563 ◽  
Author(s):  
M. Zander

Abstract Fluorescence Quenching of Alternant and Non-alternant Polycyclic Hydrocarbons by Nitro Compounds Fluorescence quenching of polycyclic aromatic hydro­ carbons by nitromethane or nitrobenzene in fluid solutions is due to an electron transfer mechanism. The non diffusion controlled rate constant of quenching is very much greater for alternant than for non-alternant hydrocarbons with equal singlet excitation energy. This is explained by the known more positive reduction potential of non-alternant compared to alternant hydrocarbons.





1981 ◽  
Vol 59 (2) ◽  
pp. 190-198 ◽  
Author(s):  
William Vidaver ◽  
Konrad Colbow ◽  
Gordon Hall ◽  
Silvia Wessel

Three distinct phases of chlorophyll a fluorescence quenching were observed in green plants by applying O2 pressures of up to 400 atm. These phases are interpreted as indications of three different mechanisms of O2 quenching. The most sensitive phase is dependent on intersystem electron transport. For dark-adapted bean leaves this fluorescence was quenched to half the initial yield with an O2 pressure of about 3 atm. The second mechanism was observed with 3-(3,4)dichlorophenyl)-1,1-dimethylurea (DCMU), namely the quenching of variable fluorescence in leaves, chloroplasts, and green algae cells. This effect of O2 is thought to be closely associated with the photochemical system II reaction centers. Half of the variable fluorescence was quenched with about 40 atm of O2. Finally, the antennae pigments are quenched, as observed by the effect of O2 on the O-level fluorescence yield, when all photochemical system II reaction center traps are presumably open. The O2 pressure required for half-quenching in this case was about 400 atm.The possibility that the quenching of fluorescence occurs with O2 concentrations low enough for endogenous O2 to have an effect on in vivo fluorescence was investigated. We suggest that O2 quenches by competing with photochemical system I for electrons derived from water splitting, and may thus function as a feedback regulator of photosynthesis.





1990 ◽  
Vol 62 (23) ◽  
pp. 2654-2656 ◽  
Author(s):  
Sadao. Matsuzawa ◽  
Akihiro. Wakisaka ◽  
Mitsuhisa. Tamura




1983 ◽  
Vol 244 (1) ◽  
pp. R15-R22 ◽  
Author(s):  
L. D. Homer ◽  
J. B. Shelton ◽  
T. J. Williams

A new method for the joint determination in tissue slices of the diffusion coefficient of oxygen (D) and the fluorescence-quenching coefficient (K') of pyrenebutyric acid (PBA) is introduced. Values of D and K' in rat brain at temperatures (Tc) from 20 to 40 degrees C were determined and referred to the values in water. D = DH2O (0.72 - 0.0074 Tc) +/- 0.079 X 10(-5) cm2/s for 80-microns slices. D = DH2O (1.01 – 0.0074 Tc) +/- 0.12 X 10(-5) cm2/s for 160-microns slices. K' = K'H2O (0.72 + 0.025 Tc) +/- 0.56 X 10(-4) mmHg-1. The temperature dependence of both these parameters in tissue is different from their temperature dependence, and comparison with parameter values obtained with other methods leads us to conclude that factors affecting the diffusion of oxygen in tissue are heterogenous rather than homogenous. The variation in the different parameters, D and K', probably exceeds a twofold range.





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