Site specific self-cleavage of certain assemblies of G-quadruplex

2008 ◽  
pp. 380-382 ◽  
Author(s):  
Xiaoqian Liu ◽  
Xinming Li ◽  
Tianyan Zhou ◽  
Yifan Wang ◽  
Magdeline Tao Tao Ng ◽  
...  
Keyword(s):  
Site Specific ◽  
G Quadruplex

scholarly journals Site-Specific and Trigger-Activated Modification of Proteins by Means of Catalytic Hemin/G-quadruplex (hGQ) DNAzyme Nanostructures

2020 ◽  
Author(s):  
Jordi Keijzer ◽  
Bauke Albada
Keyword(s):  
Heavy Chain ◽  
Protein Modification ◽  
Site Specificity ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Synthetic Dna ◽  
Drug Conjugates ◽  
Sds Page ◽  
G Quadruplex ◽  
Antibody Drug

<div>Synthetic DNA that forms various G-quadruplex nanostructures, in combination with hemin, <i>N</i>-methyl luminol derivatives, and H2O2 can site-specifically modify proteins (i.e. evidence is provided for lysozyme and human alpha-thrombin). The catalytic modification is completed in 15-30 mins, and the site-specificity is influenced by the G-quadruplex topology (a total of 22 G-quadruplex forming sequences was tested). We also show that the heavy chain of the therapeutic antibody trastuzumab is modified, which facilitates the preparation of antibody-drug conjugates. Furthermore, a trigger can be programmed into this synthetic DNA so that the protein modification chemistry is made dependent on an external trigger.</div><div><br></div>Techniques used: HPLC, SDS-PAGE, LC-MS/MS, NMR.

scholarly journals A site-specific self-assembled light-up rotor probe for selective recognition and stabilization of c-MYC G-quadruplex DNA

Nanoscale ◽  
2020 ◽  
Vol 12 (24) ◽  
pp. 12950-12957
Author(s):  
Marco Deiana ◽  
Karam Chand ◽  
Jan Jamroskovic ◽  
Rabindra Nath Das ◽  
Ikenna Obi ◽  
...  
Keyword(s):  
Selective Recognition ◽  
Site Specific ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Sensitivity And Selectivity ◽  
Self Assembled ◽  
A Site

A self-assembled light-up rotor probe with outstanding sensitivity and selectivity for the c-MYC promoter G-quadruplex DNA is reported.

scholarly journals G-quadruplex ligands exhibit differential G-tetrad selectivity

2015 ◽  
Vol 51 (38) ◽  
pp. 8048-8050 ◽  
Author(s):  
D. D. Le ◽  
M. Di Antonio ◽  
L. K. M. Chan ◽  
S. Balasubramanian
Keyword(s):  
Site Specific ◽  
Specific Fluorescence ◽  
Equilibrium Binding ◽  
Fluorescence Quench ◽  
G Quadruplex ◽  
Selective Ligands

Site-specific fluorescence quench assay allows for targeted G-quadruplex equilibrium binding measurements to investigate G-tetrad selective ligands.

scholarly journals Effects of Site-Specific Guanine C8-Modifications on an Intramolecular DNA G-Quadruplex

2011 ◽  
Vol 101 (8) ◽  
pp. 1987-1998 ◽  
Author(s):  
Christopher Jacques Lech ◽  
Joefina Kim Cheow Lim ◽  
Jocelyn Mei Wen Lim ◽  
Samir Amrane ◽  
Brahim Heddi ◽  
...  
Keyword(s):  
Site Specific ◽  
G Quadruplex

scholarly journals Site-Specific and Trigger-Activated Modification of Proteins by Means of Catalytic Hemin/G-quadruplex (hGQ) DNAzyme Nanostructures

2020 ◽  
Author(s):  
Jordi Keijzer ◽  
Bauke Albada
Keyword(s):  
Heavy Chain ◽  
Protein Modification ◽  
Site Specificity ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Synthetic Dna ◽  
Drug Conjugates ◽  
Sds Page ◽  
G Quadruplex ◽  
Antibody Drug

<div>Synthetic DNA that forms various G-quadruplex nanostructures, in combination with hemin, <i>N</i>-methyl luminol derivatives, and H2O2 can site-specifically modify proteins (i.e. evidence is provided for lysozyme and human alpha-thrombin). The catalytic modification is completed in 15-30 mins, and the site-specificity is influenced by the G-quadruplex topology (a total of 22 G-quadruplex forming sequences was tested). We also show that the heavy chain of the therapeutic antibody trastuzumab is modified, which facilitates the preparation of antibody-drug conjugates. Furthermore, a trigger can be programmed into this synthetic DNA so that the protein modification chemistry is made dependent on an external trigger.</div><div><br></div>Techniques used: HPLC, SDS-PAGE, LC-MS/MS, NMR.

A colorimetric sensing platform based on site-specific endonuclease IV-aided signal amplification for the detection of DNA related to the human immunodeficiency virus

2019 ◽  
Vol 11 (16) ◽  
pp. 2190-2196 ◽  
Author(s):  
Xiaqing Wang ◽  
Zhijun Huang ◽  
Junman Chen ◽  
Zewei Luo ◽  
Ya Xu ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Signal Amplification ◽  
Dna Detection ◽  
Colorimetric Sensing ◽  
Site Specific ◽  
Sensing Platform ◽  
Immunodeficiency Virus ◽  
G Quadruplex

A colorimetric sensing platform for DNA detection by integrating site-specific endonuclease IV-aided signal amplification and G-quadruplex-hemin DNAzyme.

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