Surface enhanced Raman spectroscopy (SERS) for the discrimination of Arthrobacter strains based on variations in cell surface composition

ABSTRACTIn this study, HeLa cells were transfected to express a recombinant transmembrane domain of the platelet-derived growth factor receptor. Silver nanoparticle (Ag-NP) functionalized with terminal hydrazide was employed to specifically target the ketone-conjugated cell surface proteins. In addition, a nitrile functional group was incorporated onto the Ag nanoprobe to provide unique vibrational signature (∼ 2230 cm−1) in a window which is free from other cellular components. The designed Ag nanoprobe functions as both recognition and transduction elements in the detection of the expressed cell surface protein. Scanning electron microscopy and surface enhanced Raman spectroscopy (SERS) imaging of the nanoparticle (NP) labeled HeLa cells revealed that all the observed intense SERS signals originated from aggregated NPs. Although isolated Ag-NPs is capable of SERS enhancement, its enhancement factor is beyond the detestability of such imaging experiments. The results have implication in the future design of SERS nanosensors for in vivo detection application.

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Confocal observation of individual classes of single wall nanotubes by surface-enhanced Raman spectroscopy

Journal de Physique IV (Proceedings) ◽

10.1051/jp4:2000864 ◽

2000 ◽

Vol 10 (PR8) ◽

pp. Pr8-223 ◽

Cited By ~ 3

Author(s):

J. Azoulay ◽

A. Débarre ◽

A. Richard ◽

P. Tchénio

Keyword(s):

Raman Spectroscopy ◽

Surface Enhanced Raman Spectroscopy ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Single Wall Nanotubes

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Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

10.26434/chemrxiv.5393281 ◽

2017 ◽

Author(s):

Caitlin S. DeJong ◽

David I. Wang ◽

Aleksandr Polyakov ◽

Anita Rogacs ◽

Steven J. Simske ◽

...

Keyword(s):

Raman Spectroscopy ◽

Bacterial Infections ◽

Direct Detection ◽

Point Of Care ◽

Surface Enhanced Raman Spectroscopy ◽

E Coli ◽

Recent Emergence ◽

Surface Enhanced ◽

Volatile Organic ◽

Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: Escherichia coli, Enterobacter cloacae, and Serratia marcescens. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of E. coli in under 12 hrs, and detected E. coli from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 103 CFU/ml and 104 CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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