scholarly journals Intracellular trafficking of cationic liposome–DNA complexes in living cells

Soft Matter ◽  
2012 ◽  
Vol 8 (30) ◽  
pp. 7919 ◽  
Author(s):  
Stefano Coppola ◽  
Laura C. Estrada ◽  
Michelle A. Digman ◽  
Daniela Pozzi ◽  
Francesco Cardarelli ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
Cationic Liposome ◽  
Living Cells ◽  
Dna Complexes

Intracellular trafficking and cytoplasmic streaming under abiotic stress conditions

2019 ◽  
Vol 6 (04) ◽  
Author(s):  
JESHIMA KHAN YASIN ◽  
ANIL KUMAR SINGH
Keyword(s):  
Plasma Membrane ◽  
Abiotic Stress ◽  
Confocal Microscopy ◽  
Fusion Protein ◽  
Intracellular Trafficking ◽  
Cytoplasmic Streaming ◽  
Living Cells ◽  
Stress Conditions ◽  
Vesicle Formation ◽  
External Stimuli

Cytoplasmic streaming is one among the vital activities of the living cells. In plants cytolplasmic streaming could clearly be seen in hypocotyls of growing seedlings. To observe cytoplsmic streaming and its correlated intracellular trafficking an investigation was conducted in legumes in comparison with GFP-AtRab75 and 35S::GFP:δTIP tonoplast fusion protein expressing arabidopsis lines. These seedlings were observed under confocal microscopy with different buffer incubation treatments and under different stress conditions. GFP expressing 35S::GFP:δTIP tonoplast lines were looking similar to the control lines and differ under stress conditions. Movement of cytoplasmic invaginations within the tonoplast and cytoplasmic sub vesicle or bulb budding during cytoplasmic streaming was observed in hypocotyls of At-GFP tonoplast plants. We found the cytoplasmic bulbs/ vesicles or sub vesicle formation from the plasma membrane. The streaming speed also depends on the incubation medium in which the specimen was incubated, indicating that the external stimuli as well as internal stimuli can alter the speed of streaming

A Columnar Phase of Dendritic Lipid−Based Cationic Liposome−DNA Complexes for Gene Delivery:  Hexagonally Ordered Cylindrical Micelles Embedded in a DNA Honeycomb Lattice

2006 ◽  
Vol 128 (12) ◽  
pp. 3998-4006 ◽  
Author(s):  
Kai K. Ewert ◽  
Heather M. Evans ◽  
Alexandra Zidovska ◽  
Nathan F. Bouxsein ◽  
Ayesha Ahmad ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Liposome ◽  
Honeycomb Lattice ◽  
Dna Complexes ◽  
Columnar Phase ◽  
Cylindrical Micelles ◽  
Hexagonally Ordered

Cationic liposome–DNA complexes (CLDC) adjuvant enhances the immunogenicity and cross-protective efficacy of a pre-pandemic influenza A H5N1 vaccine in mice

Vaccine ◽  
2012 ◽  
Vol 30 (2) ◽  
pp. 254-264 ◽  
Author(s):  
Libo Dong ◽  
Feng Liu ◽  
Jeffery Fairman ◽  
David K. Hong ◽  
David B. Lewis ◽  
...  
Keyword(s):  
Pandemic Influenza ◽  
Influenza A ◽  
Protective Efficacy ◽  
Cationic Liposome ◽  
Dna Complexes ◽  
H5n1 Vaccine

scholarly journals SNAPSwitch: A Molecular Sensor to Quantify the Localization of Proteins, DNA and Nanoparticles in Cells

2019 ◽  
Author(s):  
Laura I FitzGerald ◽  
Luigi Aurelio ◽  
Moore Chen ◽  
Daniel Yuen ◽  
Bim Graham ◽  
...  
Keyword(s):  
Immune Responses ◽  
Intracellular Trafficking ◽  
Live Cells ◽  
Quantitative Detection ◽  
Dna Complexes ◽  
Receptor Signalling ◽  
Fluorescent Markers ◽  
Molecular Sensor ◽  
Protein Nucleic Acid ◽  
In Cells

Intracellular trafficking governs receptor signalling, pathogenesis, immune responses and the cellular fate of nanomedicines. These processes are typically tracked by confocal microscopy, where colocalization of fluorescent markers implies an interaction or co-compartmentalization. However, this type of analysis is inherently low-throughput, is limited by the resolution of microscopy, and can miss fleeting interactions. To address this, we have developed a localization sensor composed of a quenched and attachable SNAP-tag substrate (SNAPSwitch). SNAPSwitch enables quantitative detection of protein, nucleic acid and nanoparticle trafficking to locations of interest within live cells using flow cytometry. Using this approach, we followed the trafficking of DNA complexes travelling from endosomes into the cytosol and to the nucleus. We also show that antibody targeted to the transferrin (CD71) or hyaluronan (CD44) receptor is initially sorted into different compartments following endocytosis. These results demonstrate SNAPSwitch is a high-throughput and broadly applicable tool to quantitatively track the localization of materials in cells.

Biophysical Characterization of Cationic Liposome–DNA Complexes and their Interaction with Cells

2003 ◽  
pp. 298-312 ◽  
Author(s):  
Maria C Pedroso de Lima ◽  
Henrique Faneca ◽  
Miguel Mano ◽  
Nuno Penacho ◽  
Nejat Düzgüneş ◽  
...  
Keyword(s):  
Cationic Liposome ◽  
Dna Complexes ◽  
Biophysical Characterization
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