Super-resolution imaging of SERS hot spots

2014 ◽  
Vol 43 (11) ◽  
pp. 3854-3864 ◽  
Author(s):  
Katherine A. Willets
Keyword(s):  
Hot Spots ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Resolution Imaging

Super-resolution imaging defeats the diffraction-limit of light, allowing the spatial origin and intensity of SERS signals to be determined with <5 nm resolution.

scholarly journals Focus on Super-Resolution Imaging with Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

2014 ◽  
Vol 67 (2) ◽  
pp. 179 ◽  
Author(s):  
Donna R. Whelan ◽  
Thorge Holm ◽  
Markus Sauer ◽  
Toby D. M. Bell
Keyword(s):  
Cell Biology ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Stochastic Optical Reconstruction Microscopy ◽  
Resolution Imaging ◽  
Optical Reconstruction ◽  
Set Up ◽  
Microscopy Techniques ◽  
Super Resolution Microscopy ◽  
Microscopic Techniques

The last decade has seen the development of several microscopic techniques capable of achieving spatial resolutions that are well below the diffraction limit of light. These techniques, collectively referred to as ‘super-resolution’ microscopy, are now finding wide use, particularly in cell biology, routinely generating fluorescence images with resolutions in the order of tens of nanometres. In this highlight, we focus on direct Stochastic Optical Reconstruction Microscopy or dSTORM, one of the localisation super-resolution fluorescence microscopy techniques that are founded on the detection of fluorescence emissions from single molecules. We detail how, with minimal assemblage, a highly functional and versatile dSTORM set-up can be built from ‘off-the-shelf’ components at quite a modest budget, especially when compared with the current cost of commercial systems. We also present some typical super-resolution images of microtubules and actin filaments within cells and discuss sample preparation and labelling methods.

scholarly journals From 2D to 3D super-resolution imaging through glass microspheres -INVITED

2020 ◽  
Vol 238 ◽  
pp. 06002
Author(s):  
Stephane Perrin ◽  
Sylvain Lecler ◽  
Paul Montgomery
Keyword(s):  
3D Reconstruction ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Label Free ◽  
Magnification Factor ◽  
Glass Microspheres ◽  
Full Field ◽  
Resolution Imaging ◽  
2D To 3D

Microsphere-assisted microscopy is a new imaging technique which allows the diffraction limit to be overcome using transparent microspheres. It makes it possible to reach a resolution of up to 100 nm in air while being label-free and full-field. An overview of the imaging technique is presented showing the influence of the photonic jet on the image nature and the unconventional behaviour of the magnification factor. Moreover, interferometry through microspheres is demonstrated for the 3D reconstruction of nanoelements.

scholarly journals Multilevel phase supercritical lens fabricated by synergistic optical lithography

Nanophotonics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1469-1477 ◽  
Author(s):  
Wei Fang ◽  
Jian Lei ◽  
Pengda Zhang ◽  
Fei Qin ◽  
Meiling Jiang ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Energy Concentration ◽  
Focal Region ◽  
Movement Trajectory ◽  
Label Free ◽  
Laser Fabrication ◽  
Resolution Imaging

AbstractThe advent of planar metalenses, including the super-oscillatory lens (SOL) and the supercritical lens (SCL) with distinctive interference properties, has profoundly impacted on the long-lasting perception of the far-field optical diffraction limit. In spite of its conspicuous success in achieving marvelously small focal spots, the planar metalens still faces tough design and fabrication challenges to realize high focusing efficiency. In this work, we demonstrated a dual-mode laser fabrication technique based on two-photon polymerization for realizing the multilevel phase SCL with focusing efficiency spiking. Synergistically controlling two types of movement trajectory, which is implemented with the piezo stage and the scanning galvo mirror, enables the fabrication of complicated structures with sub-diffraction-limit feature size. By utilizing such advantage, SCLs with discretized multilevel phase configurations are explicitly patterned. The experimental characterization results have shown that a four-level phase SCL can focus light into a sub-diffraction-limit spot with the lateral size of 0.41 λ/NA (NA is the numerical aperture), while achieve the focal spot intensity and the energy concentration ratio in the focal region 7.2 times and 3 times that of the traditional binary amplitude-type SCL with the same optimization conditions, respectively. Our results may release the application obstacles for the sub-diffraction-limit planar metalens and enable major advances in the fields from label-free optical super-resolution imaging to high precision laser fabrication.

scholarly journals Super-Resolution Imaging with Patchy Microspheres

Photonics ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 513
Author(s):  
Qingqing Shang ◽  
Fen Tang ◽  
Lingya Yu ◽  
Hamid Oubaha ◽  
Darwin Caina ◽  
...  
Keyword(s):  
Colloidal Particle ◽  
Near Field ◽  
Super Resolution ◽  
Incident Beam ◽  
Diffraction Limit ◽  
Beam Forming ◽  
Feature Size ◽  
Disc Surface ◽  
Promising Tool ◽  
Resolution Imaging

The diffraction limit is a fundamental barrier in optical microscopy, which restricts the smallest resolvable feature size of a microscopic system. Microsphere-based microscopy has proven to be a promising tool for challenging the diffraction limit. Nevertheless, the microspheres have a low imaging contrast in air, which hinders the application of this technique. In this work, we demonstrate that this challenge can be effectively overcome by using partially Ag-plated microspheres. The deposited Ag film acts as an aperture stop that blocks a portion of the incident beam, forming a photonic hook and an oblique near-field illumination. Such a photonic hook significantly enhanced the imaging contrast of the system, as experimentally verified by imaging the Blu-ray disc surface and colloidal particle arrays.

scholarly journals Recent Progress in the Correlative Structured Illumination Microscopy

Chemosensors ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 364
Author(s):  
Meiting Wang ◽  
Jiajie Chen ◽  
Lei Wang ◽  
Xiaomin Zheng ◽  
Jie Zhou ◽  
...  
Keyword(s):  
High Frequency ◽  
Optical Transmission ◽  
Imaging Technique ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Optical Diffraction ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Recent Developments ◽  
Resolution Imaging

The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

Optical Trapping, Sensing, and Imaging by Photonic Nanojets

Photonics ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 434
Author(s):  
Heng Li ◽  
Wanying Song ◽  
Yanan Zhao ◽  
Qin Cao ◽  
Ahao Wen
Keyword(s):  
Optical Tweezers ◽  
Optical Trapping ◽  
Near Field ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Research Progress ◽  
Optical Forces ◽  
Resolution Imaging ◽  
Photonic Nanojets ◽  
Near Field Scanning

The optical trapping, sensing, and imaging of nanostructures and biological samples are research hotspots in the fields of biomedicine and nanophotonics. However, because of the diffraction limit of light, traditional optical tweezers and microscopy are difficult to use to trap and observe objects smaller than 200 nm. Near-field scanning probes, metamaterial superlenses, and photonic crystals have been designed to overcome the diffraction limit, and thus are used for nanoscale optical trapping, sensing, and imaging. Additionally, photonic nanojets that are simply generated by dielectric microspheres can break the diffraction limit and enhance optical forces, detection signals, and imaging resolution. In this review, we summarize the current types of microsphere lenses, as well as their principles and applications in nano-optical trapping, signal enhancement, and super-resolution imaging, with particular attention paid to research progress in photonic nanojets for the trapping, sensing, and imaging of biological cells and tissues.

scholarly journals Imaging unlabeled proteins on DNA with super-resolution

2020 ◽  
Vol 48 (6) ◽  
pp. e34-e34 ◽  
Author(s):  
Anna E C Meijering ◽  
Andreas S Biebricher ◽  
Gerrit Sitters ◽  
Ineke Brouwer ◽  
Erwin J G Peterman ◽  
...  
Keyword(s):  
Detection Limit ◽  
Single Molecule ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Current Image ◽  
Localization Microscopy ◽  
Resolution Imaging ◽  
Biomolecular Analysis ◽  
Current Detection ◽  
Inverse Imaging

Abstract Fluorescence microscopy is invaluable to a range of biomolecular analysis approaches. The required labeling of proteins of interest, however, can be challenging and potentially perturb biomolecular functionality as well as cause imaging artefacts and photo bleaching issues. Here, we introduce inverse (super-resolution) imaging of unlabeled proteins bound to DNA. In this new method, we use DNA-binding fluorophores that transiently label bare DNA but not protein-bound DNA. In addition to demonstrating diffraction-limited inverse imaging, we show that inverse Binding-Activated Localization Microscopy or ‘iBALM’ can resolve biomolecular features smaller than the diffraction limit. The current detection limit is estimated to lie at features between 5 and 15 nm in size. Although the current image-acquisition times preclude super-resolving fast dynamics, we show that diffraction-limited inverse imaging can reveal molecular mobility at ∼0.2 s temporal resolution and that the method works both with DNA-intercalating and non-intercalating dyes. Our experiments show that such inverse imaging approaches are valuable additions to the single-molecule toolkit that relieve potential limitations posed by labeling.

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