s-Methyl cysteine enhanced survival of nerve growth factor differentiated PC12 cells under hypoxic conditions

2014 ◽  
Vol 5 (6) ◽  
pp. 1125-1133 ◽  
Author(s):  
Chun-lin Liu ◽  
Te-chun Hsia ◽  
Mei-chin Yin
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Line ◽  
Pc12 Cell ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Protective Effects ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Hypoxic Conditions

A nerve growth factor-differentiated PC12 cell line was used to investigate the protective effects ofs-methyl cysteine (SMC) at 1, 2, 4, and 8 μM under oxygen–glucose deprivation (OGD) conditions.

scholarly journals Induction of neurite outgrowth by v-src mimics critical aspects of nerve growth factor-induced differentiation.

1991 ◽  
Vol 11 (9) ◽  
pp. 4739-4750 ◽  
Author(s):  
S M Thomas ◽  
M Hayes ◽  
G D'Arcangelo ◽  
R C Armstrong ◽  
B E Meyer ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Temperature Sensitive ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Critical Aspects

PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell line was shown to elaborate neuritic processes in a temperature-inducible manner, indicating that the differentiation process was dependent on the activity of the v-src protein. Further characterization of this cell line, in comparison with NGF-treated PC12 cells, indicated that the events associated with neurite outgrowth induced by these two agents shared features but could be distinguished by others. Both NGF- and v-src-induced neurite outgrowths were reversible. In addition, NGF and v-src could prime PC12 cells for NGF-induced neurite outgrowth, and representative early and late NGF-responsive genes were also induced by v-src. However, unlike NGF-induced neurite growth, v-src-induced neurite outgrowth was not blocked at high cell density. A comparison of phosphotyrosine containing-protein profiles showed that v-src and NGF each increase tyrosine phosphorylation of multiple cellular proteins. There was overlap in substrates; however, both NGF-specific and v-src-specific tyrosine phosphorylations were observed. One protein which was found to be phosphorylated in both the NGF- and v-src-induced PC12 cells was phospholipase C-gamma 1. Taken together, these results suggest that v-src's ability to function as an inducing agent may be a consequence of its ability to mimic critical aspects of the NGF differentiation program and raise the possibility that Src-like tyrosine kinases are involved in mediating some of the events triggered by NGF.

Induction of neurite outgrowth by v-src mimics critical aspects of nerve growth factor-induced differentiation

1991 ◽  
Vol 11 (9) ◽  
pp. 4739-4750
Author(s):  
S M Thomas ◽  
M Hayes ◽  
G D'Arcangelo ◽  
R C Armstrong ◽  
B E Meyer ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Temperature Sensitive ◽  
Nerve Growth ◽  
Pc12 Cell Line ◽  
Critical Aspects

PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell line was shown to elaborate neuritic processes in a temperature-inducible manner, indicating that the differentiation process was dependent on the activity of the v-src protein. Further characterization of this cell line, in comparison with NGF-treated PC12 cells, indicated that the events associated with neurite outgrowth induced by these two agents shared features but could be distinguished by others. Both NGF- and v-src-induced neurite outgrowths were reversible. In addition, NGF and v-src could prime PC12 cells for NGF-induced neurite outgrowth, and representative early and late NGF-responsive genes were also induced by v-src. However, unlike NGF-induced neurite growth, v-src-induced neurite outgrowth was not blocked at high cell density. A comparison of phosphotyrosine containing-protein profiles showed that v-src and NGF each increase tyrosine phosphorylation of multiple cellular proteins. There was overlap in substrates; however, both NGF-specific and v-src-specific tyrosine phosphorylations were observed. One protein which was found to be phosphorylated in both the NGF- and v-src-induced PC12 cells was phospholipase C-gamma 1. Taken together, these results suggest that v-src's ability to function as an inducing agent may be a consequence of its ability to mimic critical aspects of the NGF differentiation program and raise the possibility that Src-like tyrosine kinases are involved in mediating some of the events triggered by NGF.

Effects of SARA on Oxygen–Glucose Deprivation in PC12 Cell Line

2013 ◽  
Vol 38 (5) ◽  
pp. 961-971 ◽  
Author(s):  
Jiao-Qi Wang ◽  
Jin-Ting He ◽  
Zhen-Wu Du ◽  
Zong-Shu Li ◽  
Yong-Feng Liu ◽  
...  
Keyword(s):  
Cell Line ◽  
Pc12 Cell ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Pc12 Cell Line

Local protective effects of nerve growth factor-secreting fibroblasts against excitotoxic lesions in the rat striatum

1993 ◽  
Vol 78 (2) ◽  
pp. 267-273 ◽  
Author(s):  
David M. Frim ◽  
M. Priscilla Short ◽  
William S. Rosenberg ◽  
Joseph Simpson ◽  
Xandra O. Breakefield ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Corpus Callosum ◽  
Cell Line ◽  
Neuronal Survival ◽  
Delivery Systems ◽  
Rat Striatum ◽  
Protective Effects ◽  
Nerve Growth ◽  
Excitotoxic Lesion

✓ Neurotrophic factors, such as nerve growth factor (NGF), in addition to their role in neuronal development, have protective effects on neuronal survival. Intracerebral implantation of cells genetically altered to secrete high levels of NGF is also found to promote neuronal survival in experimental lesioning models of the brain. The range of activity for such biological delivery systems has not yet been well described either spatially or temporally. Therefore, the authors chose to study the local and distant protective effects of an NGF-secreting rat fibroblast cell line implanted in an excitotoxic lesion model of Huntington's disease. They found that preimplantion of NGF-secreting fibroblasts placed within the corpus callosum reduced the maximum crosssectional area of a subsequent excitotoxic lesion in the ipsilateral striatum by 80% when compared to the effects of a non-NGF-secreting fibroblast graft, and by 83% when compared to excitotoxic lesions in ungrafted animals (p < 0.003). However, NGF-secreting cells placed in the contralateral corpus callosum failed to affect striatal lesion size significantly when compared to contralateral or ipsilateral non-NGF-secreting cell implants. Of note, fibroblasts were clearly visible within the graft site at 7 and 18 days after implantation; however, few cells within the grafts stained positively for NGF peptide or for the messenger ribonucleic acid (mRNA) encoding the transfected NGF gene-construct at either time point. These results show that biological delivery systems for NGF appear to have a profound but local effect on neuronal excitotoxicity, which will necessitate careful neurosurgical placement for maximum effect. Furthermore, the ability of this genetically altered cell line to synthesize NGF mRNA and peptide appears to decrease spontaneously in vivo, a characteristic that will need to be addressed before this method of biological delivery can be utilized as a treatment for chronic degenerative diseases.

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