Effects of SARA on Oxygen–Glucose Deprivation in PC12 Cell Line

A nerve growth factor-differentiated PC12 cell line was used to investigate the protective effects ofs-methyl cysteine (SMC) at 1, 2, 4, and 8 μM under oxygen–glucose deprivation (OGD) conditions.

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Development of an Ischemic Tolerance Model in a PC12 Cell Line

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9600003 ◽

2005 ◽

Vol 25 (2) ◽

pp. 154-162 ◽

Cited By ~ 71

Author(s):

Joëlle A Hillion ◽

Kenzo Takahashi ◽

Dragan Maric ◽

Christl Ruetzler ◽

Jeffery L Barker ◽

...

Keyword(s):

Cell Death ◽

Cell Line ◽

Pc12 Cell ◽

Annexin V ◽

Glucose Deprivation ◽

In Vitro Models ◽

Ischemic Tolerance ◽

Cellular Mechanisms ◽

Pc12 Cell Line

Although ischemic tolerance has been described in a variety of primary cell culture systems, no similar in vitro models have been reported with any cell line. A model of ischemic preconditioning in the rat pheochromocytoma PC12 cell line is described here. When compared to nonpreconditioned cells, preexposure of PC12 cells to 6 hours of oxygen and glucose deprivation (OGD) significantly increased cell viability after 15 hours of OGD 24 hours later. Flow cytometry analysis of cells labeled with specific markers for apoptosis, Annexin V, and Hoechst 33342, and of DNA content, revealed that apoptosis is involved in OGD-induced PC12 cell death and that preconditioning of the cells mainly counteracts the effect of apoptosis. Immunocytochemistry of caspase-3, a central executioner in the apoptotic process, further confirmed the activation of apoptotic pathways in OGD-induced PC12 cell death. This model may be useful to investigate the cellular mechanisms involved in neuronal transient tolerance following ischemia.

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Glucose deprivation induces mitochondrial dysfunction and oxidative stress in PC12 cell line

Journal of Cellular and Molecular Medicine ◽

10.1111/j.1582-4934.2003.tb00202.x ◽

2003 ◽

Vol 7 (1) ◽

pp. 49-56 ◽

Cited By ~ 79

Author(s):

Yan Liu ◽

Xiao-Dong Song ◽

Wen Liu ◽

Tian-Yi Zhang ◽

Ji Zuo

Keyword(s):

Oxidative Stress ◽

Mitochondrial Dysfunction ◽

Cell Line ◽

Pc12 Cell ◽

Glucose Deprivation ◽

Pc12 Cell Line ◽

And Oxidative Stress

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Establishment and Characterization of Methylmercury-Resistant PC12 Cell Line

Environmental Health Perspectives ◽

10.2307/3431810 ◽

1994 ◽

Vol 102 ◽

pp. 313 ◽

Cited By ~ 1

Author(s):

Kyoko Miura ◽

Thomas W. Clarkson ◽

Kazumasa Ikeda ◽

Akira Naganuma ◽

Nobumasa Imura

Keyword(s):

Cell Line ◽

Pc12 Cell ◽

Pc12 Cell Line

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Synaptotagmin VII Is Targeted to Dense-core Vesicles and Regulates Their Ca2+-dependent Exocytosis in PC12 Cells

Journal of Biological Chemistry ◽

10.1074/jbc.m409241200 ◽

2004 ◽

Vol 279 (50) ◽

pp. 52677-52684 ◽

Cited By ~ 58

Author(s):

Mitsunori Fukuda ◽

Eiko Kanno ◽

Megumi Satoh ◽

Chika Saegusa ◽

Akitsugu Yamamoto

Keyword(s):

Plasma Membrane ◽

Neuropeptide Y ◽

Cell Line ◽

Pc12 Cells ◽

Pc12 Cell ◽

Small Interfering Rna ◽

Dense Core ◽

Dense Core Vesicles ◽

Pc12 Cell Line ◽

Interfering Rna

It has recently been proposed that synaptotagmin (Syt) VII functions as a plasma membrane Ca2+sensor for dense-core vesicle exocytosis in PC12 cells based on the results of transient overexpression studies using green fluorescent protein (GFP)-tagged Syt VII; however, the precise subcellular localization of Syt VII is still a matter of controversy (plasma membraneversussecretory granules). In this study we established a PC12 cell line “stably expressing” the Syt VII-GFP molecule and demonstrated by immunocytochemical and immunoelectron microscopic analyses that the Syt VII-GFP protein is localized on dense-core vesicles as well as in other intracellular membranous structures, such as thetrans-Golgi network and lysosomes. Syt VII-GFP forms a complex with endogenous Syts I and IX, but not with Syt IV, and it colocalize well with Syts I and IX in the cellular processes (where dense-core vesicles are accumulated) in the PC12 cell line. We further demonstrated by an N-terminal antibody-uptake experiment that Syt VII-GFP-containing dense-core vesicles undergo Ca2+-dependent exocytosis, the same as endogenous Syt IX-containing vesicles. Moreover, silencing of Syt VII-GFP with specific small interfering RNA dramatically reduced high KCl-dependent neuropeptide Y secretion from the stable PC12 cell line (∼60% of the control cells), whereas the same small interfering RNA had little effect on neuropeptide Y secretion from the wild-type PC12 cells (∼85–90% of the control cells), indicating that the level of endogenous expression of Syt VII molecules must be low. Our results indicate that the targeting of Syt VII-GFP molecules to specific membrane compartment(s) is affected by the transfection method (transient expressionversusstable expression) and suggested that Syt VII molecule on dense-core vesicles functions as a vesicular Ca2+sensor for exocytosis in endocrine cells.

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