Sensitive colorimetric detection of melamine with 1,4-dithiothreitol modified gold nanoparticles

2015 ◽  
Vol 7 (3) ◽  
pp. 924-929 ◽  
Author(s):  
Can Xiao ◽  
Xiaofang Zhang ◽  
Junfeng Liu ◽  
Ankang Yang ◽  
Hong Zhao ◽  
...  

The presence of melamine induces a change in the state of 1,4-dithiothreitol modified gold nanoparticles, and the concentration of melamine could be quantified by the naked eye or using a UV-vis spectrometer.

The Analyst ◽  
2021 ◽  
Author(s):  
Almas Shamaila Mohammed ◽  
Aniket Balapure ◽  
Mahammad Nanne Khaja ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta

An Au NP based facile strategy for the rapid, early-stage, and sensitive detection of HCV RNA in clinical samples which avoids thiol tagging to the antisense oligonucleotide and expensive infrastructure is presented.


2013 ◽  
Vol 790 ◽  
pp. 619-622
Author(s):  
Chong Jin ◽  
Hui Min Zhao ◽  
Meng Liu ◽  
Jun Ping Tian ◽  
Xie Quan

A simple, fast and reliable colorimetric method for melamine detection based on gold nanoparticles (AuNPs) and poly-thymine (poly-T) is reported. Poly-T strands attract AuNPs through static electricity and keep them stable against aggregation in the presence of NaCl. The affinity between melamine and poly-T, hydrogen bond, frees AuNP from the protection provided by poly-T and thus makes AuNPs aggregate in the presence of NaCl. The color change of AuNPs from wine red to blue can be observed immediately by the naked eye during the aggregation process, and can be accurately recorded with the help of a UV/vis spetrophotometer within 5 minutes. The limit of detection (LOD) of melamine in pure water is 0.146 μmol/L with a linear range from 0.2×10-7 mol/L to 10.0×10-7 mol/L. In fresh milk and milk powder, the LOD is respectively as low as 2.37 μmol/L and 5.36 μmol/L.


2015 ◽  
Vol 7 (12) ◽  
pp. 4927-4933 ◽  
Author(s):  
Yi-Liang Cao ◽  
Yan Li ◽  
Fei Zhang ◽  
Jian-Zhong Huo ◽  
Xiao-Jun Zhao

A non aggregation colorimetric system was developed for the fast and sensitive detection of thiourea in aqueous environments using an unlabeled gold nanoparticle probe.


2020 ◽  
Vol 20 (4) ◽  
pp. 941
Author(s):  
Dewi Eviane ◽  
Dwi Siswanta ◽  
Sri Juari Santosa

In this study, we successfully developed gold nanoparticles capped with histidine (His-AuNPs) for Hg2+ detection using trisodium citrate as the reducing agent. The optimum pH for the detection of Hg2+ by His-AuNPs was 12. The addition of Hg2+ to the His-AuNPs caused the color change from red to black-blue, which is readily detectable by the naked eye. This color change is followed by a decrease in the intensity of the primary Surface Plasmon Resonance (SPR) peak at a wavelength (λ) of 525 nm and an increase in the secondary peak at λ = 650 nm. His-AuNPs effectively detected Hg2+ with limits of detection and quantitation of 1.77 µM and 5.89 µM, respectively. His-AuNPs exhibited good performance for the detection of Hg2+ in waste water collected from a steel industrial facility in Banten Province, with a recovery and a percent relative standard deviation of 115% and 1.02%, respectively.


2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...


2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhikun Zhang ◽  
Xiaojie Ye ◽  
Qingqing Liu ◽  
Yumin Liu ◽  
Runjing Liu

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