A surface plasmon resonance assay coupled with a hybridization chain reaction for amplified detection of DNA and small molecules

2014 ◽  
Vol 50 (39) ◽  
pp. 5049-5052 ◽  
Author(s):  
Xuemei Li ◽  
Yan Wang ◽  
Linlin Wang ◽  
Qingli Wei
Keyword(s):  
Surface Plasmon Resonance ◽  
Small Molecules ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Biological Samples ◽  
Detection System ◽  
High Sensitivity ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A surface plasmon resonance (SPR) detection system based on a hybridization chain reaction (HCR) was developed for amplified detection of DNA and small molecules with high sensitivity. This methodology is capable of detecting the target in complicated biological samples and can be further extended to the detection of other proteins or biomarkers.

scholarly journals Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 75
Author(s):  
Ching-Hsu Yang ◽  
Tzu-Heng Wu ◽  
Chia-Chen Chang ◽  
Hui-Yun Lo ◽  
Hui-Wen Liu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Dna Sequences ◽  
Plasmon Resonance ◽  
High Efficiency ◽  
Specific Binding ◽  
Dna Amplification ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Phase Sensitive

Surface Plasmon Resonance (SPR) is widely used in biological and chemical sensing with fascinating properties. However, the application of SPR to detect trace targets is hampered by non-specific binding and poor signal. A variety of approaches for amplification have been explored to overcome this deficiency including DNA aptamers as versatile target detection tools. Hybridization chain reaction (HCR) is a high-efficiency enzyme-free DNA amplification method operated at room temperature, in which two stable species of DNA hairpins coexist in solution until the introduction of the initiator strand triggers a cascade of hybridization events. At an optimal salt condition, as the concentrations of H1 and H2 increased, the HCR signals were enhanced, leading to signal amplification reaching up to 6.5-fold of the detection measure at 30 min. This feature enables DNA to act as an amplifying transducer for biosensing applications to provide an enzyme-free alternative that can easily detect complex DNA sequences. Improvement of more diverse recognition events can be achieved by integrating HCR with a phase-sensitive SPR (pSPR)-tested aptamer stimulus. This work seeks to establish pSPR aptamer system for highly informative sensing by means of an amplification HCR. Thus, combining pSPR and HCR technologies provide an expandable platform for sensitive biosensing.

High Sensitivity of Phase-based Surface Plasmon Resonance in Nano-cylinder Array

PIERS Online ◽  
2008 ◽  
Vol 4 (7) ◽  
pp. 746-750 ◽  
Author(s):  
Bing-Hung Chen ◽  
Yih-Chau Wang ◽  
Jia-Hng Lin
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Sensitivity ◽  
Cylinder Array

scholarly journals Reusable TiN Substrate for Surface Plasmon Resonance Heterodyne Phase Interrogation Sensor

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1325 ◽  
Author(s):  
Ru-Jing Sun ◽  
Hung Ji Huang ◽  
Chien-Nan Hsiao ◽  
Yu-Wei Lin ◽  
Bo-Huei Liao ◽  
...  
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Sensitivity ◽  
Nanorod Array ◽  
Glass Substrates ◽  
Sensing Applications ◽  
Bulk Charge ◽  
Phase Interrogation

A TiN-based substrate with high reusability presented high-sensitivity refractive index measurements in a home-built surface plasmon resonance (SPR) heterodyne phase interrogation system. TiN layers with and without additional inclined-deposited TiN (i-TiN) layers on glass substrates reached high bulk charge carrier densities of 1.28 × 1022 and 1.91 × 1022 cm−3, respectively. The additional 1.4 nm i-TiN layer of the nanorod array presented a detection limit of 6.1 × 10−7 RIU and was higher than that of the 46 nm TiN layer at 1.2 × 10−6 RIU when measuring the refractive index of a glucose solution. Furthermore, the long-term durability of the TiN-based substrate demonstrated by multiple processing experiments presented a high potential for various practical sensing applications.

High-Sensitivity Optical-Fiber Microfluidic Chip Based on Surface Plasmon Resonance

2021 ◽  
Vol 48 (1) ◽  
pp. 0106002
Author(s):  
李钢敏 Li Gangmin ◽  
李致远 Li Zhiyuan ◽  
李正冉 Li Zhengran ◽  
王锦民 Wang Jinmin ◽  
夏历 Xia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Microfluidic Chip ◽  
Plasmon Resonance ◽  
High Sensitivity
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