Self-assembled DNA nanostructures prepared by rolling circle amplification for the delivery of siRNA conjugates

2014 ◽  
Vol 50 (86) ◽  
pp. 13049-13051 ◽  
Author(s):  
Cheol Am Hong ◽  
Bora Jang ◽  
Eun Hye Jeong ◽  
Hansaem Jeong ◽  
Hyukjin Lee
Keyword(s):  
Large Scale ◽  
Rolling Circle Amplification ◽  
Sirna Delivery ◽  
Dna Nanostructures ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Large Scale Preparation ◽  
Amplification Process ◽  
Scale Preparation ◽  
Self Assembled

Large-scale preparation of DNA nanostructures for siRNA delivery has been achieved by an isothermal enzymatic amplification process.

Efficient Self‐Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in v itro

2019 ◽  
Vol 37 (6) ◽  
pp. 588-592 ◽  
Author(s):  
Qian Yao ◽  
Yuqi Chen ◽  
Fan Wu ◽  
Fan Wu ◽  
Chaoxing Liu ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Sirna Delivery ◽  
Rolling Circle ◽  
Dna Nanoparticles ◽  
Self Assembled

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography

1992 ◽  
Vol 70 (6) ◽  
pp. 448-454 ◽  
Author(s):  
Ewa Świeżewska ◽  
T. Chojnacki ◽  
W. J. Jankowski ◽  
K. Singh ◽  
J. Olsson
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Large Scale ◽  
Fatty Acid Esters ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Wet Weight ◽  
Rosaceae Family ◽  
Acid Esters ◽  
Long Chain

The long chain polyprenols composed of 30 and more isoprene units from leaves of plants belonging to the genera Potentilla and Rosa have been described. They occur in the form of fatty acid esters. The composition of polyprenol mixture was species dependent and its content reached ca. 0.5% wet weight. Large scale preparation of individual polyprenols from a natural polyprenol mixture was performed using time-extended liquid chromatography on the hydrophobic gel Lipidex-5000.Key words: long chain polyprenols, Rosaceae.

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