Mineralized biomimetic collagen/alginate/silica composite scaffolds fabricated by a low-temperature bio-plotting process for hard tissue regeneration: fabrication, characterisation and in vitro cellular activities

2014 ◽  
Vol 2 (35) ◽  
pp. 5785 ◽  
Author(s):  
HyeongJin Lee ◽  
YongBok Kim ◽  
SuHon Kim ◽  
GeunHyung Kim
Keyword(s):  
Low Temperature ◽  
Tissue Regeneration ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Silica Composite

scholarly journals In Vitro and Ex Vivo Analysis of Collagen Foams for Soft and Hard Tissue Regeneration

In Vivo ◽  
2021 ◽  
Vol 35 (5) ◽  
pp. 2541-2549
Author(s):  
OLE JUNG ◽  
MIKE BARBECK ◽  
LU FAN ◽  
FABIAN KORTE ◽  
CUIFENG ZHAO ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Ex Vivo ◽  
Hard Tissue

3D-printed alginate/phenamil composite scaffolds constituted with microsized core–shell struts for hard tissue regeneration

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29335-29345 ◽  
Author(s):  
KyoungHo Lee ◽  
Cho-Rong Seo ◽  
Jin-Mo Ku ◽  
Hyeongjin Lee ◽  
Hyeon Yoon ◽  
...  
Keyword(s):  
3D Printing ◽  
Tissue Regeneration ◽  
Composite Scaffold ◽  
Core Shell ◽  
Coating Process ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Combined Process ◽  
3D Printed

A new composite scaffold consisting of poly(ε-caprolactone), alginate, and phenamil was manufactured by a combined process, 3D-printing and coating process, for hard tissue regeneration.

In Vitro Osteogenic Activity of Rat Mesenchymal Cells Cultured on Transparent β-Tricalcium Phosphate Ceramics

2005 ◽  
Vol 284-286 ◽  
pp. 663-666 ◽  
Author(s):  
Noriko Kotobuki ◽  
Koji Ioku ◽  
Daisuke Kawagoe ◽  
Daishiro Nomura ◽  
Hirotaka Fujimori ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Microscopic Observation ◽  
Mesenchymal Cells ◽  
Hard Tissue ◽  
Osteogenic Activity ◽  
Light Microscopic Observation ◽  
Mineralized Matrix ◽  
Excellent Property

We have cultured mesenchymal cells (MSC) on various types of ceramic disks and used these tissue-engineered ceramics for hard tissue regeneration. In this approach, observation of cultured cell morphology is important even if culture substrata are calcium phosphate ceramics, which usually show bioactive nature. However, due to the opaque nature of the ceramics, cells observation is very difficult. Here, we demonstrate light microscopic observation of rat MSC cultured on transparent β-tricalcium phosphate ceramics (β-TCP). The culture was performed in osteogenic medium. Thus, the cell differentiated into bone-forming osteoblasts, which fabricated a mineralized matrix on the ceramic disks. Microscopic observation revealed that the cascade of osteogenic differentiation after attachment/proliferation of MSC on the ceramic disks was similar to that on a culture grade polystyrene dish. These results confirmed the excellent property of β-TCP for MSC culture leading to hard tissue regeneration.

scholarly journals Factorial Study of Compressive Mechanical Properties and PrimaryIn VitroOsteoblast Response of PHBV/PLLA Scaffolds

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Naznin Sultana ◽  
Tareef Hayat Khan
Keyword(s):  
Mechanical Properties ◽  
Polymer Matrix ◽  
Tissue Regeneration ◽  
Degradation Rate ◽  
Bone Tissue Regeneration ◽  
Osteoblastic Cells ◽  
Composite Scaffolds ◽  
Biomimetic Systems ◽  
Human Osteoblastic Cells

For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polymer matrix to control the degradation rate. In order to render the scaffolds osteoconductive, nano-sized hydroxyapatite (nHA) particles can be incorporated into the polymer matrix. In the first part of this study, a factorial design approach to investigate the influence of materials on the initial compressive mechanical properties of the scaffolds was studied. In the second part, the protein adsorption behavior and the attachment and morphology of osteoblast-like cells (Saos-2) of the scaffoldsin vitrowere also studied. It was observed that nHA incorporated PHBV/PLLA composite scaffolds adsorbed more bovine serum albumin (BSA) protein than PHBV or PHBV/PLLA scaffolds.In vitrostudies also revealed that the attachment of human osteoblastic cells (SaOS-2) was significantly higher in nHA incorporated PHBV/PLLA composite scaffolds. From the SEM micrographs of nHA incorporated PHBV/PLLA composite scaffolds seeded with SaOS-2 cells after a 7-day cell culture period, it was observed that the cells were well expanded and spread in all directions on the scaffolds.

Fabrication and in vitro biocompatibilities of fibrous biocomposites consisting of PCL and M13 bacteriophage-conjugated alginate for bone tissue engineering

2016 ◽  
Vol 4 (4) ◽  
pp. 656-665 ◽  
Author(s):  
Jae Yoon Lee ◽  
Jinhyo Chung ◽  
Woo-Jae Chung ◽  
GeunHyung Kim
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Hard Tissue ◽  
M13 Bacteriophage ◽  
Bioactive Component ◽  
M13 Phage

We propose a new biocomposite consisting of M13-phage-conjugated alginate and PCL micro/nanofibers, and it has potential to be used as an excellent bioactive component for hard tissue regeneration.

Sign in / Sign up

Export Citation Format

Share Document