Spectrophotometric and spectrofluorimetric determination of mesna, acetylcysteine and timonacic acid through the reaction with acetoxymercuri fluorescein

2016 ◽  
Vol 8 (11) ◽  
pp. 2479-2493 ◽  
Author(s):  
Rim S. Haggag ◽  
Dina A. Gawad ◽  
Saeid F. Belal ◽  
Hadil M. Elbardisy
Keyword(s):  
Spectrophotometric Method ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Sulfur Containing ◽  
Timonacic Acid

Simple, sensitive and specific spectrophotometric (Method I) and spectrofluorimetric (Method II) methods were developed for the determination of three sulfur-containing drugs: mesna (MSN), acetylcysteine (ACT) and timonacic acid (TMN).

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

Spectrofluorimetric determination of uric acid based on its activation of catalytic oxidation of pyronine Y

2008 ◽  
Vol 62 (3) ◽  
Author(s):  
Suling Feng ◽  
Xueping Liu
Keyword(s):  
Uric Acid ◽  
Buffer Solution ◽  
Activation Effect ◽  
Buffer Solutions ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Uric Acid Determination ◽  
Catalyzed Oxidation ◽  
Acid Determination

AbstractA novel kinetic spectrofluorimetric method for the determination of uric acid based on the activation effect of uric acid on the Cu(II) ion catalyzed oxidation of pyronine Y by hydrogen peroxide was developed. The influence of different buffer solutions was tested and the Britton-Robinson buffer solution with pH 2.2 was found to be the optimum. The detection limit and the linear range for uric acid are 0.09 μg mL−1 and 0.3–3.0 μg mL−1, respectively. The RSD for eleven determinations of 1.6 μg mL−1 uric acid was 1.6 %. Satisfactory results were obtained when using this method of uric acid determination in human urine.

Application of a quinone-based fluorophore for spectrofluorimetric determination of albendazole in pure form and pharmaceutical formulations

2016 ◽  
Vol 8 (25) ◽  
pp. 5136-5141 ◽  
Author(s):  
Khalid A. M. Attia ◽  
Ahmad A. Mohamad ◽  
Mohamed S. Emara
Keyword(s):  
Charge Transfer ◽  
Complex Formation ◽  
Pharmaceutical Formulations ◽  
Pure Form ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Ct Complex

A spectrofluorimetric method was developed for the determination of albendazole (ALB) through charge transfer (CT) complex formation with 7,7,8,8-tetracyanoquinodimethane (TCNQ).

Spectrofluorimetric Determination of Bilirubin Using Enoxacine-Terbium Probe

2014 ◽  
Vol 556-562 ◽  
pp. 421-424 ◽  
Author(s):  
Wei Wei Bian
Keyword(s):  
Detection Limit ◽  
Fluorescence Intensity ◽  
Trace Amount ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Buffer Solution ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Terbium Ion

A new spectrofluorimetric method was developed for determination of trace amount of bilirubin. Using enoxacine–terbium ion as a fluorescent probe, in the buffer solution of pH=5.8, BR can remarkably reduce the fluorescence intensity of the ENX-Tb3+ complex at λ=545nm and the reduced fluorescence intensity of Tb3+ ion is in proportion to the concentration of BR. Optimum conditions for the determination of BR were also investigated. The linear range and detection limit for the determination of BR are 1.0×10-7～4.5×10-6mol/L and 8.1×10-8mol/L. This method is simple, practical and can be successfully applied to assess BR in serum samples.

scholarly journals Spectrofluorimetric determination of amlodipine in human plasma without derivatization

2012 ◽  
Vol 48 (4) ◽  
pp. 719-725 ◽  
Author(s):  
Yucel Kadioglu ◽  
Murat Ozturk
Keyword(s):  
Channel Blocker ◽  
Limit Of Detection ◽  
Plasma Samples ◽  
Experimental Conditions ◽  
Spectrofluorimetric Method ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Spectrofluorimetric Determination ◽  
Better Than

A rapid and sensitive spectrofluorimetric method was developed for the determination of amlodipine (AD), a calcium channel blocker, in the plasma. The type of solvent, the wavelength range, and the range of AD concentration were selected to optimize the experimental conditions. The calibration curves were linear (r² >0.997) in the concentration range of 0.1-12.5 ppm of AD. The limit of quantitation and limit of detection values for the method for plasma samples were 0.1 ppm and 0.07 ppm, respectively. The precision calculated as the relative standard deviation was less than 3.5%, and the accuracy (relative error) was better than 5.5% (n=6). The method developed in this study can be directly and easily applied for the determination of AD in the plasma without derivatization in plasma.

scholarly journals Spectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent

2021 ◽  
Vol 22 (2) ◽  
pp. 367-375
Author(s):  
Theia'a Najim Al-Sabha ◽  
Mohamed Yahya Dhamra
Keyword(s):  
Fluorescence Intensity ◽  
Rhodamine 6G ◽  
Pharmaceutical Preparations ◽  
Detection Limits ◽  
Ion Pair ◽  
Amino Groups ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Tertiary Amino

A sensitive spectrofluorimetric method has been developed for the analysis of some medicines containing primary, secondary, and tertiary amino groups, namely Diclofenac (DIC), Domperidone (DOM), Famotidine (FAM), and Propranolol (PRO), in their pure and medicinal forms. The method is based on the quenching of the fluorescence intensity of rhodamine 6G (R6G) through the formation of ion-pair complexes between the above medicines and the R-6G reagent, which is measured at 552 nm after excitation at 402 nm. The calibration graphs were rectilinear in the concentration ranges of 0.10- 9.00, 0.05-15.00, 0.10-14.0 and 0.05-5.00 µg mL-1 for above medicines respectively. The recovery (%) values were ranged between 99.45%- 100.97%. The detection limits ranged in the concentration of 0.243-0.754 µg/mL, and the limits of quantitation were 0.806- 2.420 µgmL-1 for all drugs. The method was successfully applied for the determination of these drugs in their pharmaceutical preparations.

scholarly journals Spectrofluorimetric determination of carvedilol in dosage form and spiked human plasma through derivatization with 1-dimethylaminonaphthalene-5-sulphonyl chloride

2010 ◽  
Vol 16 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Fattah El-Sayed ◽  
Taghreed Mohamed ◽  
Anwer Taha
Keyword(s):  
Human Plasma ◽  
Reaction Pathway ◽  
Biological Fluid ◽  
Spiked Human Plasma ◽  
Spectrofluorimetric Method ◽  
Limit Of Quantitation ◽  
Spectrofluorimetric Determination ◽  
Yield Formation ◽  
Good Agreement

A sensitive, simple and selective spectrofluorimetric method was developed for the determination of carvedilol (CA) in pharmaceutical formulation and a biological fluid. The method is based on the reaction between the drug and 1-dimethylaminonaphthalene- 5-sulphonyl chloride (dansyl chloride) in the presence of mixture (acetone:0.5 M sodium carbonate, 3:2) at pH 10 to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 350 nm. Different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The fluorescence concentration plot was rectilinear over the range of 5.0-80.0 ng ml-1 with a lower detection limit (LOD) of 1.90 ng ml-1 and the limit of quantitation (LOQ) of 5.15 ng ml-1. Quantum yield, formation constant (K) and free energy change (?G) values were calculated. The proposed method was successfully applied to the analysis of commercial tablets. The results obtained were in good agreement with those obtained using the official titrimetric method [1,2]. Furthermore, the method was applied for the determination of CA in spiked human plasma, the mean % recovery (n = 5) is 97.82?0.373. A proposal of the reaction pathway was presented.

scholarly journals Spectrofluorimetric determination of Bisoprolol fumarate and Rosuvastatin calcium in a novel combined formulation and in human spiked plasma

2018 ◽  
Vol 9 (4) ◽  
pp. 331-337 ◽  
Author(s):  
Nada Sayed Abdelwahab ◽  
Nouruddin Wageh Ali ◽  
Marco Mounir Zaki ◽  
Adel Ahmed Ali ◽  
Mohamed Mohamed Abdelkawy
Keyword(s):  
Simultaneous Determination ◽  
High Sensitivity ◽  
Emission Wavelength ◽  
Bioanalytical Method Validation ◽  
Spiked Plasma ◽  
Spectrofluorimetric Method ◽  
Significant Difference ◽  
Spectrofluorimetric Determination ◽  
Rosuvastatin Calcium

Sensitive, simple and rapid spectrofluorimetric method was developed for simultaneous determination of bisoprolol fumarate (BIS) and rosuvastatin calcium (ROS) in novel formulated tablets and in human spiked plasma depending on measuring their native fluorescence. The fluorescence intensity of BIS and ROS were measured in methanol at emission wavelength of 297 and 485 nm upon excitation at 227 and 242 nm, respectively. The emission spectrum of each drug reveals zero value at the emission wavelength of the other drug, thus allowing their simultaneous determination without any interference and without using any tedious derivatization steps. Excellent linearity was obtained over the range of 10-500 and 20-1000 ng/mL for BIS and ROS, respectively. The developed method was evaluated by applying to laboratory prepared mixtures and pharmaceutical formulation. The high sensitivity of the method was the motivation to its application for analysis of the cited drugs in spiked human plasma. Likewise, analytical and bioanalytical method validation was carried out following International Conference on Harmonisation guidelines and also statistical analysis with the reported methods was carried out and no significant difference was found. The developed method is the first developed spectrofluorimetric method for simultaneous determination of the newly formulated drugs.

scholarly journals Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms

2014 ◽  
Vol 33 (2) ◽  
pp. 209 ◽  
Author(s):  
Leposava Pavun ◽  
Predrag Đurđević ◽  
Milena Jelikić-Stankov ◽  
Daniela Đikanović ◽  
Andrija Ćirić ◽  
...  
Keyword(s):  
Direct Determination ◽  
Dosage Forms ◽  
Font Size ◽  
Pharmaceutical Dosage Forms ◽  
Strong Emission ◽  
Rp Hplc ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Good Agreement

<p><span style="font-size: medium;"><span style="font-family: Times New Roman;">The simple, accurate and precise method based on fluorescence properties of aluminium (III)–quercetin complex, for the determination of quercetin has been developed and validated. The complex has strong emission at pH 3.30, </span><em>l</em></span><span style="font-family: Times New Roman;"><span style="font-size: small;"><em><sub>em</sub></em></span><span style="font-size: medium;"> = 480 nm, with </span></span><span style="font-size: medium;"><em>l</em></span><span style="font-family: Times New Roman;"><span style="font-size: small;"><em><sub>ex</sub></em></span><span style="font-size: medium;"> = 420 nm</span><span style="font-size: medium;">. Linearity range of quercetin determination was 1.5 - 60.5 ng mL</span><sup><span style="font-size: small;">-1</span></sup><span style="font-size: medium;"> with LOD 0.09 ng mL</span><sup><span style="font-size: small;">-1</span></sup><span style="font-size: medium;"> and LOQ 0.27 ng mL</span><sup><span style="font-size: small;">-1</span></sup><span style="font-size: medium;">. Recovery values in the range of 99.9 – 100.2 % indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with </span><span style="font-size: medium;">Recovery value<strong> </strong>98.3 %, standard deviation 0.22 <strong>% </strong>and<strong> </strong>coefficient of variation<strong> </strong>0.09<strong> %</strong>.<strong> </strong></span></span></p><p>  The reliability of the method was checked by RP-HPLC/UV method for capsules with direct determination of quercetin after separation. The good agreement between two methods indicates the applicability usability of the proposed spectroflurometric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables direct and simple determination without its prior extraction from samples.</p><p>The proposed spectrofluorimetric method has much better sensitivity and about 1000 times lower <em>LOD</em> and <em>LOQ</em> values compared to data reported in literature.</p>

Determination of nitrite in cheese and other dairy products

1972 ◽  
Vol 39 (1) ◽  
pp. 89-94 ◽  
Author(s):  
C. G. Rammell ◽  
M. M. Joerin
Keyword(s):  
Uv Irradiation ◽  
Spectrophotometric Method ◽  
Dairy Products ◽  
Raw Milk ◽  
Spectrofluorimetric Method

SummaryDetails are given of a spectrophotometric method for determining nitrite in cheese and other dairy products at levels down to 0·05 μg NO2-N/g and of a spectrofluorimetric method for levels down to 0·003 μg NO2-N/g. Nitrite-N levels in cheese varied from 0·003 μg/g to 0·021 μg/g. These levels increased up to 4-fold after UV irradiation of the cheese extracts. No nitrite (< 0·003 μg/ml) could be detected in raw milk.

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