scholarly journals Label-free detection of DNA single-base mismatches using a simple reflectance-based optical technique

2016 ◽  
Vol 18 (19) ◽  
pp. 13395-13402 ◽  
Author(s):  
G. Nava ◽  
E. Ceccarello ◽  
F. Giavazzi ◽  
M. Salina ◽  
F. Damin ◽  
...  
Keyword(s):  
Optical Technique ◽  
Label Free ◽  
Dna Oligomers ◽  
Single Base ◽  
Label Free Detection ◽  
Single Sequence

Reflective Phantom Interface, a novel label-free optical technique, allows to detect and quantify the hybridization of DNA oligomers at a surface, easily discriminating single sequence mismatches.

scholarly journals Label-Free Detection of Single-Base Mismatches in DNA by Surface-Enhanced Raman Spectroscopy

2011 ◽  
Vol 123 (39) ◽  
pp. 9224-9227 ◽  
Author(s):  
Evanthia Papadopoulou ◽  
Steven E. J. Bell
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Single Base ◽  
Label Free Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission

2011 ◽  
Vol 78 (2) ◽  
pp. 747-752 ◽  
Author(s):  
Dan Tang ◽  
Ping Lu ◽  
Dongli Liao ◽  
Xiangyu Yang ◽  
Yujing Zhang ◽  
...  
Keyword(s):  
Label Free ◽  
Excimer Emission ◽  
Single Base ◽  
Single Base Mismatch ◽  
Label Free Detection ◽  
Base Mismatch

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

scholarly journals Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1872
Author(s):  
Holger Schulze ◽  
Harry Wilson ◽  
Ines Cara ◽  
Steven Carter ◽  
Edward N. Dyson ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Electrochemical Impedance ◽  
Point Of Care ◽  
Branched Polymers ◽  
Label Free ◽  
Conformation Change ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Staphylococcus Carnosus ◽  
Label Free Detection

Rapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.

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