Novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid) porous scaffolds fabricated by phase separation for tissue engineering applications

Three-dimensional polymer scaffolds with interconnected porous structures were fabricated by thermally induced phase separation of novel biodegradable poly(propylene fumarate)-co-poly(l-lactic acid).

Download Full-text

Recent Progress on Biodegradable Tissue Engineering Scaffolds Prepared by Thermally-Induced Phase Separation (TIPS)

International Journal of Molecular Sciences ◽

10.3390/ijms22073504 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3504

Author(s):

Reza Zeinali ◽

Luis J. del Valle ◽

Joan Torras ◽

Jordi Puiggalí

Keyword(s):

Tissue Engineering ◽

Phase Separation ◽

Material Selection ◽

Three Dimensional ◽

Target Cells ◽

Porous Scaffolds ◽

Thermally Induced Phase Separation ◽

Tissue Engineering Scaffolds ◽

Thermally Induced ◽

Biodegradable Scaffolds

Porous biodegradable scaffolds provide a physical substrate for cells allowing them to attach, proliferate and guide the formation of new tissues. A variety of techniques have been developed to fabricate tissue engineering (TE) scaffolds, among them the most relevant is the thermally-induced phase separation (TIPS). This technique has been widely used in recent years to fabricate three-dimensional (3D) TE scaffolds. Low production cost, simple experimental procedure and easy processability together with the capability to produce highly porous scaffolds with controllable architecture justify the popularity of TIPS. This paper provides a general overview of the TIPS methodology applied for the preparation of 3D porous TE scaffolds. The recent advances in the fabrication of porous scaffolds through this technique, in terms of technology and material selection, have been reviewed. In addition, how properties can be effectively modified to serve as ideal substrates for specific target cells has been specifically addressed. Additionally, examples are offered with respect to changes of TIPS procedure parameters, the combination of TIPS with other techniques and innovations in polymer or filler selection.

Download Full-text

Preparation of Three-Dimensional Scaffolds from Degradable Poly(ether)esterurethane by Thermally-Induced Phase Separation

Macromolecular Symposia ◽

10.1002/masy.201100051 ◽

2011 ◽

Vol 309-310 (1) ◽

pp. 76-83 ◽

Cited By ~ 1

Author(s):

Karola Luetzow ◽

Thomas Weigel ◽

Michael Schossig ◽

Karl Kratz ◽

Andreas Lendlein

Keyword(s):

Phase Separation ◽

Three Dimensional ◽

Thermally Induced Phase Separation ◽

Thermally Induced

Download Full-text

Structure–property relation of porous poly (l-lactic acid) scaffolds fabricated using organic solvent mixtures and controlled cooling rates and its bio-compatibility with human adipose stem cells

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911518758354 ◽

2018 ◽

Vol 33 (4) ◽

pp. 397-415 ◽

Cited By ~ 2

Author(s):

Harish Chinnasami ◽

Jeff Gimble ◽

Ram V Devireddy

Keyword(s):

Stem Cells ◽

Cell Proliferation ◽

Lactic Acid ◽

Phase Separation ◽

Mechanical Strength ◽

Separation Method ◽

Thermally Induced Phase Separation ◽

Cooling Rates ◽

Thermally Induced ◽

Pore Sizes

Thermally induced phase separation method was used to make porous three-dimensional poly (l-lactic acid) scaffolds. The effect of imposed thermal profile during freezing of the poly (l-lactic acid) in dioxane solution on the scaffold was characterized by their micro-structure, porosity (%), pore sizes’ distribution, and mechanical strength. The porosity (%) decreased considerably with increasing concentrations of poly (l-lactic acid) in the solution, while a decreasing trend was observed with increasing cooling rates. The mechanical strength increases with increase in poly (l-lactic acid) concentration and also with increase in the cooling rate for both types of solvents. Therefore, mechanical strength was increased by higher cooling rates while the porosity (%) remained relatively consistent. Scaffolds made using higher concentrations of poly (l-lactic acid; 7% and 10% w/v) in solvent showed better mechanical strength which improved relatively with increasing cooling rates (1°C–40°C/min). This phenomenon of enhanced structural integrity with increasing cooling rates was more prominent in scaffolds made from higher initial poly (l-lactic acid) concentrations. Human adipose–derived stem cells were cultured on these scaffold (7% and 10% w/v) prepared by thermally induced phase separation at all cooling rates to measure the cell proliferation efficiency as a function of their micro-structural properties. Mean pore sizes played a crucial role in cell proliferation than percent porosity since all scaffolds were >88% porous. The viability percent of human adipose tissue–derived adult stem cells increased consistently with longer periods of culture. Thus, poly (l-lactic acid) scaffolds prepared by thermally controlled thermally induced phase separation method could be a prime candidate for making ex vivo tissue-engineered grafts for surgical implantation.

Download Full-text

Specially elaborated thermally induced phase separation to fabricate poly(L-lactic acid) scaffolds with ultra large pores and good interconnectivity

Journal of Applied Polymer Science ◽

10.1002/app.23931 ◽

2006 ◽

Vol 101 (5) ◽

pp. 3336-3342 ◽

Cited By ~ 55

Author(s):

Yihong Gong ◽

Zuwei Ma ◽

Changyou Gao ◽

Wei Wang ◽

Jiacong Shen

Keyword(s):

Lactic Acid ◽

Phase Separation ◽

Thermally Induced Phase Separation ◽

Thermally Induced

Download Full-text

Comparison of Supermacroporous Polyester Matrices Fabricated by Thermally Induced Phase Separation and 3D Printing Techniques

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.822.277 ◽

2019 ◽

Vol 822 ◽

pp. 277-283

Author(s):

Mariia Stepanova ◽

Aleksei Eremin ◽

Ilia Averianov ◽

Iosif Gofman ◽

Antonina Lavrentieva ◽

...

Keyword(s):

Phase Separation ◽

3D Printing ◽

Three Dimensional ◽

Uniaxial Compression Test ◽

Thermally Induced Phase Separation ◽

Cell Penetration ◽

Thermally Induced ◽

Printing Technique ◽

3D Printing Technique ◽

Printing Techniques

Supermacroporous three-dimensional matrices based on poly-D,L-lactide or polycaprolactone were fabricated by thermally induced phase separation method and 3D printing technique. The morphology and mechanical properties of the resulting matrices were studied with the use of optical and scanning electron microscopy and the uniaxial compression test, respectively. All matrices were characterized with supermacroporous structure suitable for cell penetration. A significant increase in Young's modulus and tensile strength was established for both polymer matrices prepared by 3D printing technique.

Download Full-text